Differential role of neurokinin receptors in human lymphocyte and monocyte chemotaxis

Hood, Vivienne C.; Cruwys, Simon; Urbán, László; Kidd, Bruce L.

doi:10.1016/s0167-0115(00)00195-6

Cited by 32 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…58 This idea has merit, because under conditions of inflammation SP is elevated locally in tissues (including tears of patients with pterygia) 58,59 and systemically in plasma, 60 where it functions as a chemoattractant for immune cells. 61 Although our observations support SP as a chemoattractant for a population of unidentified mononuclear cells (which could include EPCs), the absence of NK 1 receptor staining in the vasculature of pterygia, conflicts with our cell migration data. It is possible that NK 1 receptors became upregulated in vascular endothelium under cell culture conditions or through immortalization.…”

Section: Discussioncontrasting

confidence: 80%

The Role of Substance P in the Pathogenesis of Pterygia

Chui

Girolamo

Coroneo

et al. 2007

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 80%

The Role of Substance P in the Pathogenesis of Pterygia

Chui

Girolamo

Coroneo

et al. 2007

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…The vascular actions of CGRP and SP are well known: they promote neurogenic inflammation and aid the extravasation of leukocytes from the bloodstream 67,68 . CGRP and SP have also been shown to have direct chemotactic activity on leukocytes of both the innate and adaptive immune systems [75][76][77][78][79][80] . Moreover, they can modulate leukocyte recruitment by mobilizing adhesion molecules to the endothelial cell surface [81][82][83] .…”

Section: Actions Of Neuropeptides On Immune Cellsmentioning

confidence: 99%

Crosstalk between the nociceptive and immune systems in host defence and disease

2015

View full text Add to dashboard Cite

Nociceptors and immune cells both protect the host from potential threats to homeostasis. There is growing evidence for bidirectional signalling between these two systems, and the underlying mechanisms are beginning to be elucidated. An understanding is emerging of how both the adaptive and innate immune systems can activate and sensitize nociceptors, and, reciprocally, how nociceptors modulate immune cells. In this Review, we discuss how these interactions can be adaptive and useful to the organism but also consider when such signalling might be maladaptive and pathophysiological, contributing to immune-mediated diseases and persistent pain states.

show abstract

“…Although the existence and expression of NK1R-F and NK1R-T have been documented in several cell types [32,38,39,41], the differences in their expression and physiological function have not been studied in human peripheral blood monocytes. Several studies have identified NK1R in human cells of monocyte/macrophage lineage; however, it is less certain which form of the receptor is expressed in human monocytes [5,9,18,21,24,42,43]. It has been variously reported that human monocytes express NK1R, do not express NK1R, or express an atypical form of the receptor [5,28,44,45].…”

Section: Introductionmentioning

confidence: 99%

Substance P (SP) enhances CCL5-induced chemotaxis and intracellular signaling in human monocytes, which express the truncated neurokinin-1 receptor (NK1R)

Chernova¹,

Lai²,

Li³

et al. 2008

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Substance P (SP) is a potent modulator of monocyte/macrophage function. The SP-preferring receptor neurokinin-1 receptor (NK1R) has two forms: a full-length NK1R (NK1R-F) isoform and a truncated NK1R (NK1R-T) isoform, which lacks the terminal cytoplasmic 96-aa residues. The distribution of these receptor isoforms in human monocytes is not known. We previously identified an interaction among SP, NK1R, and HIV viral strains that use the chemokine receptor CCR5 as a coreceptor, suggesting crosstalk between NK1R and CCR5. The purpose of this study was to determine which form(s) of NK1R are expressed in human peripheral blood monocytes and to determine whether SP affects proinflammatory cellular responses mediated through the CCR5 receptor. Human peripheral blood monocytes were found to express NK1R-T but not NK1R-F. SP interactions with NK1R-T did not mobilize calcium (Ca2+), but SP mobilized Ca2+ when the NK1R-F was transfected into monocytes. However, the NK1R-T was functional in monocytes, as SP enhanced the CCR5 ligand CCL5-elicited Ca2+ mobilization, a response inhibited by the NK1R antagonist aprepitant. SP interactions with the NK1R-T also enhanced CCL5-mediated chemotaxis, which was ERK1/2-dependent. NK1R-T selectively activated ERK2 but increased ERK1 and ERK2 activation by CCL5. Activation of NK1R-T elicited serine phosphorylation of CCR5, indicating that crosstalk between CCL5 and SP may occur at the level of the receptor. Thus, NK1R-T is functional in human monocytes and activates select signaling pathways, and the NK1R-T-mediated enhancement of CCL5 responses does not require the NK1R terminal cytoplasmic domain.

show abstract

Differential role of neurokinin receptors in human lymphocyte and monocyte chemotaxis

Cited by 32 publications

References 27 publications

The Role of Substance P in the Pathogenesis of Pterygia

The Role of Substance P in the Pathogenesis of Pterygia

Crosstalk between the nociceptive and immune systems in host defence and disease

Substance P (SP) enhances CCL5-induced chemotaxis and intracellular signaling in human monocytes, which express the truncated neurokinin-1 receptor (NK1R)

Contact Info

Product

Resources

About