Differential RNA Expression Profile by cDNA Microarray in Sporadic Primary Hyperparathyroidism (pHPT): Primary Parathyroid Hyperplasia versus Adenoma

Velázquez-Fernández, David; Laurell, Cecilia; Saqui‐Salces, Milena; Pantoja, Juan Pablo; Candanedo-González, Fernando; Reza-Albarrán, Alfredo; Gamboa‐Domínguez, Armando; Herrera, Miguel F.

doi:10.1007/s00268-005-0708-3

Cited by 21 publications

(23 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Telomerase activity is mainly regulated at the level of hTERT gene transcription, and number of activators and inhibitors influence the hTERT promoter, including c-Myc, Sp1, estrogen receptor (ERalpha) and p53. 20, 21 WRAP53 mRNA is increased in cancer cells 22, 23 indicating that upregulation of WRAP53 protein is caused by enhanced transcription, however, the responsible factors influencing the WRAP53 promoter remain to be identified. Notably, MCF10A cells were also more sensitive to WRAP53 depletion in comparison with the primary cells, suggesting that as the cells become more cancerous the more important WRAP53 levels are for their survival.…”

Section: Discussionmentioning

confidence: 99%

WRAP53 promotes cancer cell survival and is a potential target for cancer therapy

Mahmoudi

Henriksson

Farnebo³

et al. 2011

Cell Death Dis

View full text Add to dashboard Cite

We previously identified WRAP53 as an antisense transcript that regulates the p53 tumor suppressor. The WRAP53 gene also encodes a protein essential for Cajal body formation and involved in cellular trafficking of the survival of motor neuron complex, the telomerase enzyme and small Cajal body-specific RNAs to Cajal bodies. Here, we show that the WRAP53 protein is overexpressed in a variety of cancer cell lines of different origin and that WRAP53 overexpression promotes cellular transformation. Knockdown of the WRAP53 protein triggers massive apoptosis through the mitochondrial pathway, as demonstrated by Bax/Bak activation, loss of mitochondrial membrane potential and cytochrome c release. The apoptosis induced by WRAP53 knockdown could moreover be blocked by Bcl-2 overexpression. Interestingly, human tumor cells are more sensitive to WRAP53 depletion as compared with normal human cells indicating that cancer cells in particular depends on WRAP53 expression for their survival. In agreement with this, we found that high levels of WRAP53 correlate with poor prognosis of head and neck cancer. Together these observations propose a role of WRAP53 in carcinogenesis and identify WRAP53 as a novel molecular target for a large fraction of malignancies.

show abstract

Section: Discussionmentioning

confidence: 99%

WRAP53 promotes cancer cell survival and is a potential target for cancer therapy

Mahmoudi

Henriksson

Farnebo³

et al. 2011

Cell Death Dis

View full text Add to dashboard Cite

show abstract

“…WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. Unfortunately, there is not much literature on the function of WDR79 , although we know that the protein has been reported to be upregulated in parathyroid [51] and brain tumors [52] . Also, the discovery that silencing of this locus results in massive cell apoptosis has led to the patenting of a new method of treatment of hyperproliferative conditions (i.e., cancer) (International Application No.…”

Section: Discussionmentioning

confidence: 99%

The Diversity Profile of <i>TP53</i> Is Influenced by Positive Selection on the Immediately Upstream Locus<i> WDR79</i>

Alonso

Izagirre²,

López³

et al. 2009

Hum Hered

View full text Add to dashboard Cite

Background/Aim:TP53 is an efficient central node in a signal transduction network that responds to minimize cancer. However, over 50% of tumors show some mutation in TP53. Thus, one might argue that this single central node network lacks robustness. Therefore, we wanted to investigate if natural selection has played a role in shaping the genomic region containing TP53. Methods: We have analyzed the HapMap data for evidence of selection using F_ST pairwise comparisons and the extended haplotype homozygosity test on a 200-kb region encompassing TP53. We have also resequenced 4 kb upstream TP53 in Europeans (including melanoma patients), Asians, Australian Aborigines and Africans. Results: Genetic hitchhiking by a linked, positively selected allele at the nearby gene WDR79 may be partly responsible for the sequence diversity profile of TP53. It can help explain why the TP53 Arg72 allele is the major allele in Europeans even when the alternative allele, 72Pro, has been reported to offer an increased longevity after disease. Conclusions: Despite the important role of TP53, a complex interplay with other evolutionary forces, which are extrinsic to TP53 function, may have driven the genetic diversity pattern of this locus, and, as a consequence, its structure and function.

show abstract

“…Microarray analysis of tumor transcriptomes is a powerful technology that is capable of assessing the expression levels of thousands of genes simultaneously in one sample. It has been used to explore the molecular mechanisms and the genetic etiologies of PHPT and SHPT [39,40]. Forsberg et al [41] used microarray analysis to compare gene expression in parathyroid adenomas and normal parathyroid glands.…”

Section: Pathogenesismentioning

confidence: 99%

Parathyroid Carcinoma: Current Understanding and New Insights into Gene Expression and Intraoperative Parathyroid Hormone Kinetics

2010

View full text Add to dashboard Cite

After completing this course, the reader will be able to:1. Discuss the association of parathyroid cancers with severe hypercalcemia and markedly elevated parathyroid hormone levels.2. Describe the effect of the risk factors, clinical presentation, and gene expression on diagnosis of parathyroid cancers.3. Analyze the probability of cure for parathyroid cancers with treatment by aggressive surgery with en bloc resection and lymph node dissection.This article is available for continuing medical education credit at CME.TheOncologist.com. The Oncologist CME Program is located online at http://cme.theoncologist.com/. To take the CME activity related to this article, you must be a registered user. CME CME ABSTRACT EndocrinologyThe

show abstract

Differential RNA Expression Profile by cDNA Microarray in Sporadic Primary Hyperparathyroidism (pHPT): Primary Parathyroid Hyperplasia versus Adenoma

Abstract: Our data suggest that SPH and adenoma have a singular molecular signature that, theoretically, could be used for the differential diagnosis of these entities and normal parathyroid tissue.

Cited by 21 publications

References 62 publications

WRAP53 promotes cancer cell survival and is a potential target for cancer therapy

WRAP53 promotes cancer cell survival and is a potential target for cancer therapy

The Diversity Profile of <i>TP53</i> Is Influenced by Positive Selection on the Immediately Upstream Locus<i> WDR79</i>

Parathyroid Carcinoma: Current Understanding and New Insights into Gene Expression and Intraoperative Parathyroid Hormone Kinetics

Contact Info

Product

Resources

About