Differential response to Cadmium exposure by expression of a two and a three-domain metallothionein isoform in the land winkle Pomatias elegans: Valuating the marine heritage of a land snail

Schmielau, Lara; Dvorak, Martin; Niederwanger, Michael; Dobieszewski, Nicole; Pedrini-Martha, Veronika; Ladurner, Peter; Pedregal, Jaime Rodríguez-Guerra; Maréchal, Jean‐Didier; Dallinger, Reinhard

doi:10.1016/j.scitotenv.2018.07.426

Cited by 15 publications

(17 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…48 The selective advantage that MTs with high metal-binding capacity might provide to a marine animal as O. dioica is still unknown, but it has been suggested that the evolution of multi-domain MTs in some animal species (e.g. Littorina littorea and Pomatias elegans with 3-domain MTs [49][50][51] Crassostrea virginica with 4-domain MTs, 52 and Alinda biplicata with 10-domain MTs 53 ) might have improved their response to metal stress and adverse environmental conditions, offering a selective advantage to organisms that experience a greater exposure to metals due to their ecological niche. 50,52…”

Section: Identification Structural Features and Evolutionary Origin mentioning

confidence: 99%

Metallothioneins of the urochordateOikopleura dioicahave Cys-rich tandem repeats, large size and cadmium-binding preference

et al. 2018

View full text Add to dashboard Cite

show abstract

Section: Identification Structural Features and Evolutionary Origin mentioning

confidence: 99%

Metallothioneins of the urochordateOikopleura dioicahave Cys-rich tandem repeats, large size and cadmium-binding preference

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Sample digestion and analysis of Cd tissue concentrations were performed as described previously [27]. Shortly, a 1:1 mixture of nitric acid (65%) (Suprapur, Merck, Darmstadt, Germany) and deionized water were pipetted into lidded Eppendorf tubes containing the oven-dried samples (multi-tissue samples, snail cookies, and reference material) and digested under pressure in a heated (70 • C) aluminum block.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of N1, the aberrance can be explained by the shorter length of this domain due to a missing linker region in the front (Figure 4). Overall, the structural organization of the md-MTs of A. biplicata is closely related to that of the three-domain MTs of Littorina littorea and Pomatias elegans [26,27], which can both be regarded as md-MTs with only one repetition of the N-terminal metal-binding domain. Apart from that, it has also been demonstrated that the C-terminal metal binding domains are highly conserved among all snail MTs.…”

Section: Characterization Of Multi-domain Mt Genes In Alinda Biplicatamentioning

confidence: 92%

“…More recent studies demonstrate, however, a greater variability of gastropod MT protein structures than so far known. The marine periwinkle Littorina littorea and its terrestrial relative Pomatias elegans, for example, have increased the metal-binding capacity of their Cd-selective MTs by addition of a third Cd-binding domain, derived from a duplication of their N-terminal domain [26,27]. Consequently, these newly discovered three-domain MTs have increased their binding stoichiometry from 6 to 9 Cd 2+ ions [26,28].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cadmium Uptake, MT Gene Activation and Structure of Large-Sized Multi-Domain Metallothioneins in the Terrestrial Door Snail Alinda biplicata (Gastropoda, Clausiliidae)

Pedrini-Martha

Köll

Dvorak

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Terrestrial snails (Gastropoda) possess Cd-selective metallothioneins (CdMTs) that inactivate Cd2+ with high affinity. Most of these MTs are small Cysteine-rich proteins that bind 6 Cd2+ equivalents within two distinct metal-binding domains, with a binding stoichiometry of 3 Cd2+ ions per domain. Recently, unusually large, so-called multi-domain MTs (md-MTs) were discovered in the terrestrial door snail Alinda biplicata (A.b.). The aim of this study is to evaluate the ability of A.b. to cope with Cd stress and the potential involvement of md-MTs in its detoxification. Snails were exposed to increasing Cd concentrations, and Cd-tissue concentrations were quantified. The gene structure of two md-MTs (9md-MT and 10md-MT) was characterized, and the impact of Cd exposure on MT gene transcription was quantified via qRT PCR. A.b. efficiently accumulates Cd at moderately elevated concentrations in the feed, but avoids food uptake at excessively high Cd levels. The structure and expression of the long md-MT genes of A.b. were characterized. Although both genes are intronless, they are still transcribed, being significantly upregulated upon Cd exposure. Overall, our results contribute new knowledge regarding the metal handling of Alinda biplicata in particular, and the potential role of md-MTs in Cd detoxification of terrestrial snails, in general.

show abstract

“…Vertebrate and most other animal MTs exhibit a dumbbell-shaped three-dimensional structure with two metal binding domains, each containing a rigid core holding three to four metal ions [9]. Some snail species like Littorina littorea and Pomatias elegans, express three-domain MTs [10,11], which were not known until recently.…”

Section: Introductionmentioning

confidence: 99%

Cadmium Pathways in Snails Follow a Complementary Strategy between Metallothionein Detoxification and Auxiliary Inactivation by Phytochelatins

Dvorak

Schnegg

Niederwanger

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Metal detoxification is crucial for animals to cope with environmental exposure. In snails, a pivotal role in protection against cadmium (Cd) is attributed to metallothioneins (MTs). Some gastropod species express, in a lineage-specific manner, Cd-selective MTs devoted exclusively to the binding and detoxification of this single metal, whereas other species of snails possess non-selective MTs, but still show a high tolerance against Cd. An explanation for this may be that invertebrates and in particular snails may also synthetize phytochelatins (PCs), originally known to be produced by plants, to provide protection against metal or metalloid toxicity. Here we demonstrate that despite the fact that similar mechanisms for Cd inactivation exist in snail species through binding of the metal to MTs, the actual detoxification pathways for this metal may follow different traits in a species-specific manner. In particular, this depends on the detoxification capacity of MTs due to their Cd-selective or non-specific binding features. In the terrestrial slug Arion vulgaris, for example, Cd is solely detoxified by a Cd-selective MT isoform (AvMT1). In contrast, the freshwater snail Biomphalaria glabrata activates an additional pathway for metal inactivation by synthesizing phytochelatins, which compensate for the insufficient capacity of its non-selective MT system to detoxify Cd. We hypothesize that in other snails and invertebrate species, too, an alternative inactivation of the metal by PCs may occur, if their MT system is not Cd-selective enough, or its Cd loading capacity is exhausted.

show abstract

Differential response to Cadmium exposure by expression of a two and a three-domain metallothionein isoform in the land winkle Pomatias elegans: Valuating the marine heritage of a land snail

Cited by 15 publications

References 62 publications

Metallothioneins of the urochordateOikopleura dioicahave Cys-rich tandem repeats, large size and cadmium-binding preference

Metallothioneins of the urochordateOikopleura dioicahave Cys-rich tandem repeats, large size and cadmium-binding preference

Cadmium Uptake, MT Gene Activation and Structure of Large-Sized Multi-Domain Metallothioneins in the Terrestrial Door Snail Alinda biplicata (Gastropoda, Clausiliidae)

Cadmium Pathways in Snails Follow a Complementary Strategy between Metallothionein Detoxification and Auxiliary Inactivation by Phytochelatins

Contact Info

Product

Resources

About