Differential response of Staphylococci and osteoblasts to varying titanium surface roughness

Wu, Yong; Zitelli, Joseph P.; TenHuisen, Kevor S.; Yu, Xiaojun; Libera, Matthew

doi:10.1016/j.biomaterials.2010.10.001

Cited by 219 publications

(201 citation statements)

References 46 publications

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“…In addition, a significant increase in the number of nodules and the amount of mineralized matrix are detected within certain realms of surface roughness. Our results are consistent with the observations of Wu et al 35 in that microroughened titanium with rough surfaces enhances ALP activity and extracellular calcium deposition over conventional smooth titanium implants. However, the peak of mineralized nodules emerges on the group 2 surface, and a striking decreased tendency in cell mineralization is seen in group 3, underlining the role of moderate surface roughness in the enhancement of osteogenic differentiation efficiency.…”

Section: In Vitro Osteogenic Differentiation Responsesupporting

confidence: 93%

Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite

Deng¹,

Liu²,

Xu³

et al. 2015

IJN

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As United States Food and Drug Administration-approved implantable material, carbon fiber-reinforced polyetheretherketone (CFRPEEK) possesses an adjustable elastic modulus similar to cortical bone and is a prime candidate to replace surgical metallic implants. The bioinertness and inferior osteogenic properties of CFRPEEK, however, limit its clinical application as orthopedic/dental implants. In this study, CFRPEEK–nanohydroxyapatite ternary composites (PEEK/n-HA/CF) with variable surface roughness have been successfully fabricated. The effect of surface roughness on their in vitro cellular responses of osteoblast-like MG-63 cells (attachment, proliferation, apoptosis, and differentiation) and in vivo osseointegration is evaluated. The results show that the hydrophilicity and the amount of Ca ions on the surface are significantly improved as the surface roughness of composite increases. In cell culture tests, the results reveal that the cell proliferation rate and the extent of osteogenic differentiation of cells are a function of the size of surface roughness. The composite with moderate surface roughness significantly increases cell attachment/proliferation and promotes the production of alkaline phosphatase (ALP) activity and calcium nodule formation compared with the other groups. More importantly, the PEEK/n-HA/CF implant with appropriate surface roughness exhibits remarkably enhanced bioactivity and osseointegration in vivo in the animal experiment. These findings will provide critical guidance for the design of CFRPEEK-based implants with optimal roughness to regulate cellular behaviors, and to enhance biocompability and osseointegration. Meanwhile, the PEEK/n-HA/CF ternary composite with optimal surface roughness might hold great potential as bioactive biomaterial for bone grafting and tissue engineering applications.

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Section: In Vitro Osteogenic Differentiation Responsesupporting

confidence: 93%

Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite

Deng¹,

Liu²,

Xu³

et al. 2015

IJN

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“…However, ALP activity was significantly reduced with the immobilization of fibronectin on nanometersmooth surfaces. Wu et al 34) reported that ALP level of a polished surface with nanometer smoothness was much higher than that of grit-blasted surfaces at 16 days of cell culture. Although the detailed mechanism will be further investigated, the effect of combining sandblasting and FN immobilization may contribute to the improvement of ALP activity for T-sand/FN.…”

Section: Proliferation and Attached Cell Morphologymentioning

confidence: 99%

Biochemical surface modifications to titanium implants using the tresyl chlorideactivated method

Hayakawa

2015

Dent. Mater. J.

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Tresyl chloride (2,2,2-trifluoroethanesulfonyl chloride)-activated method is an easy and simple method for immobilizing biological molecules onto a titanium surface. Tresyl chloride was directly dropped onto a titanium surface without any solvent. Tresylated titanium disks were immersed in a protein or peptide solution to give protein-or peptide-immobilized titanium. Frontier molecular orbital calculations suggested that tresylation of the OH compound improved its reactivity towards the amine groups of protein compounds. Quartz-crystal microbalance-dissipation measurements suggested that ionic interaction between the oxygen of the terminal hydroxyl groups of titanium and the nitrogen of fibronectin was important for fibronectin immobilization. Initial cell attachment was enhanced by fibronectin or collagen immobilization. Fibronectin and fibronectin-derived peptide immobilization improved the expression of specific genes related to bone formation. The immobilization of cytokine onto titanium enhanced bone formation following its implantation into rat femur defects. The tresyl chloride-activated method is useful for immobilizing biological molecules onto titanium surfaces.

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“…Ti with micro and nano scale surface roughness (Truong et al, 2010) S. epidermidis Ti with four modified different roughness (Wu et al, 2011) …”

Section: S Aureus P Aeruginosamentioning

confidence: 99%

Oral Bacterial Adhesion and Biocompatibility of Silver-Amorphous Carbon Films: A Surface Modification for Dental Implants

Almaguer-Flore¹,

Rodil²,

Olivares-Navarrete³

2011

Implant Dentistry - The Most Promising Discipline of Dentistry

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Differential response of Staphylococci and osteoblasts to varying titanium surface roughness

Cited by 219 publications

References 46 publications

Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite

Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite

Biochemical surface modifications to titanium implants using the tresyl chlorideactivated method

Oral Bacterial Adhesion and Biocompatibility of Silver-Amorphous Carbon Films: A Surface Modification for Dental Implants

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Differential response of Staphylococci and osteoblasts to varying titanium surface roughness

Cited by 219 publications

References 46 publications

Effect of surface roughness on osteogenesis in&nbsp;vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone&ndash;nanohydroxyapatite composite

Effect of surface roughness on osteogenesis in&nbsp;vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone&ndash;nanohydroxyapatite composite

Biochemical surface modifications to titanium implants using the tresyl chlorideactivated method

Oral Bacterial Adhesion and Biocompatibility of Silver-Amorphous Carbon Films: A Surface Modification for Dental Implants

Contact Info

Product

Resources

About

Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite

Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite