Differential response of Dunaliella salina and Dunaliella tertiolecta isolated from brines of Sambhar Salt Lake of Rajasthan (India) to salinities: A study on growth, pigment and glycerol synthesis

Arun, N.; Singh, Divya

doi:10.6024/jmbai.2013.55.1.01758-11

Cited by 12 publications

(4 citation statements)

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“…It is inferred that dramatic changes in living conditions can bring massively unique bioactive proteins and peptides different from those of terrestrial plants and other microalgae [17–19]. Fortunately, some researchers have confirmed that, due to salt stress and flagellar disassembly, peroxiredoxin mRNA and protein expression significantly increased in Dunaliella salina cells [20–22], while salt stress has also been found to contribute to accumulation of unique bioactive proteins [23]. Thus, the information seemingly gives some light on the antioxidant activities of protein and peptide derived from this microalgae.…”

Section: Introductionmentioning

confidence: 99%

Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

Xia

Zhai

Huang

et al. 2019

BioMed Research International

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DPPH• scavenging peptides (<3kDa) from underutilized Dunaliella salina protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated in vitro gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L−1 NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated in vitro digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of Dunaliella salina could be a potential source of antioxidative peptides through simulated in vitro gastrointestinal digestion.

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Section: Introductionmentioning

confidence: 99%

Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

Xia

Zhai

Huang

et al. 2019

BioMed Research International

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“…Indeed, a high cell viability ensures proliferation and a higher resistance in the transformation process (Pratheesh et al, 2014). Moreover, cell kinetic parameters, specific growth rate (μ= 0.167 day −1 ) and doubling time (dt = 4.14 day), were found to be better than the reported previously by Arun and Singh (2013), who showed values of μ = 0.13 day −1 and dt = 5.8 day for the same D. tertiolecta. Higher values of μ indicates better ability of an organism to grow under a given set of environmental conditions; while, lower dt values indicate shorter time (days) required for cells to divide.…”

Section: Discussionmentioning

confidence: 62%

Establishment of an efficient genetic transformation method in Dunaliella tertiolecta mediated by Agrobacterium tumefaciens

Norzagaray-Valenzuela

Germán-Báez

Valdez-Flores

et al. 2018

Journal of Microbiological Methods

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Microalgae are photosynthetic microorganisms widely used for the production of highly valued compounds, and recently they have been shown to be promising as a system for the heterologous expression of proteins. Several transformation methods have been successfully developed, from which the Agrobacterium tumefaciens-mediated method remains the most promising. However, microalgae transformation efficiency by A. tumefaciens is shown to vary depending on several transformation conditions. The present study aimed to establish an efficient genetic transformation system in the green microalgae Dunaliella tertiolecta using the A. tumefaciens method. The parameters assessed were the infection medium, the concentration of the A. tumefaciens and co-culture time. As a preliminary screening, the expression of the gusA gene and the viability of transformed cells were evaluated and used to calculate a novel parameter called Transformation Efficiency Index (TEI). The statistical analysis of TEI values showed five treatments with the highest gusA gene expression. To ensure stable transformation, transformed colonies were cultured on selective medium using hygromycin B and the DNA of resistant colonies were extracted after five subcultures and molecularly analyzed by PCR. Results revealed that treatments which use solid infection medium, A. tumefaciens OD = 0.5 and co-culture times of 72 h exhibited the highest percentage of stable gusA expression. Overall, this study established an efficient, optimized A. tumefaciens-mediated genetic transformation of D. tertiolecta, which represents a relatively easy procedure with no expensive equipment required. This simple and efficient protocol opens the possibility for further genetic manipulation of this commercially-important microalgae for biotechnological applications.

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“…They demonstrated that glycerol was induced in the cells with maximum values of 30.5 pg cell −1 for D. salina and 19.36 ± 0.13 pg cell −1 for D. tertiolecta achieved at 30% and 25% NaCl concentration, respectively. So, they concluded that under low concentration of salt (around 10%), negligible amounts of glycerol would be accumulated in D. salina with a mean value of about 5.46 pg cell −1 (Arun & Singh, 2013). In the present study, a similar level of salinity (8%) induced similar amounts of glycerol in D. salina in a PEG-free control with a a mean value of 6.74 pg cell −1 .…”

Section: Discussionmentioning

confidence: 99%

Stress responses of the green microalga, Dunaliella salina to PEG-induced drought

et al. 2020

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Drought stress was evaluated with polyethylene glycol 6000 (PEG 6000) treatment in Dunaliella salina, a microalga known for its great ability to withstand salinities of more than 30%. The aim was to explore the acclimation mechanisms used by the microalga to regulate its growth and physiology during coping with drought stress. The microalga was subjected to culture mediums containing 2 and 5% PEG for 25 days and was compared with a control culture medium. Significant decrease in growth parameters such as specific growth rate, biomass and number of divisions per day was demonstrated in PEG-treated algae. During PEG treatment, chlorophylls slightly increased, while β-carotene and total protein were not affected. Osmolytes, as well as carbohydrates, were found to be significantly higher in PEG-treated algae than in control. Increased catalase and ascorbate peroxidase activities were proportionally related to PEG concentrations in the cultures. The PEG-treated cells accumulated a considerable amount of hydrogen peroxide and malondialdehyde, especially at higher PEG concentrations. Electrolyte leakage increased, regardless of the PEG concentrations applied, while DNA fragmentation was not observed after 25 days of treating with PEG. It was concluded that Dunaliella cells could respond to the drought stress, probably by using a higher accumulation of a range of osmolytes and also more stimulation of the antioxidant enzymatic system.

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Differential response of Dunaliella salina and Dunaliella tertiolecta isolated from brines of Sambhar Salt Lake of Rajasthan (India) to salinities: A study on growth, pigment and glycerol synthesis

Cited by 12 publications

References 0 publications

Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

Establishment of an efficient genetic transformation method in Dunaliella tertiolecta mediated by Agrobacterium tumefaciens

Stress responses of the green microalga, Dunaliella salina to PEG-induced drought

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