Differential Regulation of Uncoupling Protein-2 and Uncoupling Protein-3 Gene Expression in Brown Adipose Tissue during Development and Cold Exposure

Carmona, Mamen; Valmaseda, Angel; Brun, Sònia; Viñas, Octavi; Mampel, Teresa; Iglesias, Roser; Giralt, Marta; Villarroya, Francesc

doi:10.1006/bbrc.1998.8088

Cited by 50 publications

(46 citation statements)

References 23 publications

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“…In agreement with this, we did not find elevated UCP2 expression in tissues of mice exposed to 4 mC for 16 h [30]. However, other groups reported an increased UCP2 mRNA level in BAT of mice exposed to the cold for 5 h [206], or in BAT, heart and skeletal muscle of rats exposed to 5 mC for 2 days [207]. An up-regulation of UCP2 and UCP3 mRNAs was reported in interscapular BAT of rats exposed to 4 mC for 7 days [208].…”

Section: A Major Role For Ucp1 Stucp and Atucp In Response To Cold Esupporting

confidence: 92%

“…In vivo j cold-exposed rats by Larkin et al [178], whereas other authors noted no effect of cold on UCP3 gene expression in mice [206]. There is agreement that cold exposure does not activate UCP3 mRNA expression in skeletal muscle, indicating that this protein is not primarily implicated in body temperature regulation [84,178,207].…”

Section: Ucp1mentioning

confidence: 97%

“…In itro and in i o experiments demonstrated that fatty acids or certain retinoids promote UCP2 and\or UCP3 expression in white and brown adipocytes, myocytes and pancreatic islets [171,172,177,206,[229][230][231][232][233][234][235][236][237][238]. Expression of UCP2 and\or UCP3 in skeletal muscles, heart or adipose tissue can be induced by various situations characterized by elevated levels of circulating non-esterified fatty acids, such as starvation in humans or rodents [84,169,239,240], high-fat feeding in rodents [30,177] and genetic or experimental diabetes in rodents [31,241,242].…”

Section: Contribution Of Ucps To Dit Energy Partitioning and Lipid Mmentioning

confidence: 98%

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The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

Ricquier¹,

Bouillaud

2000

Biochemical Journal

610

115

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Animal and plant uncoupling protein (UCP) homologues form a subfamily of mitochondrial carriers that are evolutionarily related and possibly derived from a proton\anion transporter ancestor. The brown adipose tissue (BAT) UCP1 has a marked and strongly regulated uncoupling activity, essential to the maintenance of body temperature in small mammals. UCP homologues identified in plants are induced in a cold environment and may be involved in resistance to chilling. The biochemical activities and biological functions of the recently identified mammalian UCP2 and UCP3 are not well known. However, recent data support a role for these UCPs in State 4 respiration, respiration uncoupling and proton leaks in mitochondria. Moreover, genetic studies suggest that UCP2 and UCP3 play a part in

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Section: A Major Role For Ucp1 Stucp and Atucp In Response To Cold Esupporting

confidence: 92%

Section: Ucp1mentioning

confidence: 97%

Section: Contribution Of Ucps To Dit Energy Partitioning and Lipid Mmentioning

confidence: 98%

See 1 more Smart Citation

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

Ricquier¹,

Bouillaud

2000

Biochemical Journal

610

115

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“…The results of these studies suggested that the gene expression of both UCPs were similarly modified by those factors that occur after feeding a high-energy diet. In this context, it has been shown that UCP2 mRNA levels were increased after ␤ 3 -agonist treatment [10] and cold exposure [12,34]. However, in contrast to UCP1 and UCP3, the expression levels of UCP2 in iBAT were not modified after feeding a highenergy diet.…”

Section: Discussionmentioning

confidence: 98%

Changes in UCP mRNA expression levels in brown adipose tissue and skeletal muscle after feeding a high-energy diet and relationships with leptin, glucose and PPARγ

Margareto

Marti

Martínéz

2001

The Journal of Nutritional Biochemistry

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Brown adipose tissue and skeletal muscle are known to be important sites for nonshivering thermogenesis. In this context, it is accepted that uncoupling proteins (UCPs) are involved in such process, but little is known about the physiological regulation of these proteins as affected by the intake of a high-energy (cafeteria) diet inducing fat deposition. In this study, the UCP messenger RNA (mRNA) expression in interscapular brown adipose tissue (iBAT) and skeletal muscle was assessed to evaluate the influence of a dietary manipulation on energy homeostasis regulation. We report a statistically significant increase in mRNA levels of iBAT UCP1 and UCP3 and a statistical marginal rise in skeletal muscle UCP3 mRNA expression after feeding a high-energy diet, whereas no changes in UCP2 expression were found in either tissue. Furthermore, significant positive associations between iBAT UCP1 and UCP3 mRNA levels with serum leptin were found. Although the expression of the ␤ 3 adrenoceptor (␤ 3 AR) was about 50% in the lean controls compared with the obese group in iBAT, no statistically significant changes were observed concerning peroxisome proliferator-activated receptor ␥2 (PPAR␥2) mRNA levels in muscle or iBAT. We conclude that feeding a diet inducing weight and fat gain produces different outcomes on iBAT and skeletal muscle UCP mRNA expression, revealing a tissue-dependent response for the three UCPs. Results suggest that the regulation of UCP expression in both tissues under these specific dietary conditions may be related to leptin circulating levels.

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“…UCPs are thought to transport fatty acid anions from an inner surface to an outer surface of the inner mitochondrial membrane to release stored energy as heat without coupling to other energyconsuming process [5][6][7]. The heat production generated mainly from BAT and skeletal muscle in rodents plays important roles, especially in newborn mammals, and in arousal from hibernation [1,8]. These facts suggest that UCPs contribute to controlling energy balance.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of ubiquitous expression of mouse uncoupling protein 2 mRNA: control by cis-acting DNA element in 5′-flanking region

Yoshitomi¹,

Yamazaki

Tanaka

1999

Biochemical Journal

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Uncoupling protein (UCP) 2 is a member of the uncouplingprotein family, and it appears to function as an uncoupler of oxidative phosphorylation. To identify cis-acting regulatory elements controlling this gene's expression, we cloned an approx. 6.2-kb region upstream from the translation-initiation site of the mouse UCP2 gene and analysed its transcription activity using chimaeric mouse UCP2 promoter-placental-alkaline-phosphatase (PLAP) reporter-gene constructs. Sequence analysis showed that the 5h-flanking region of the mouse UCP2 gene was not similar to those of mouse UCP1 or UCP3. For the mouse UCP2, the region near the transcription-initiation site lacked the typical TATA box, but was GC-rich, resulting in presence of several potential specificity protein 1 (Sp-1), activator protein (AP)-1 and AP-2 binding sites. The putative regulatory motifs for muscle-regulatory protein (MyoD), brown-fat regulatory element, CCAAT box, cAMP-response element and Y box were also found in the mouse UCP2 promoter region by computerassisted analysis. From the results of Northern-blot analysis and transient expression assay, we found that the mouse UCP2 gene responded to the cAMP-dependent protein kinase α-catalytic

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Differential Regulation of Uncoupling Protein-2 and Uncoupling Protein-3 Gene Expression in Brown Adipose Tissue during Development and Cold Exposure

Cited by 50 publications

References 23 publications

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

Changes in UCP mRNA expression levels in brown adipose tissue and skeletal muscle after feeding a high-energy diet and relationships with leptin, glucose and PPARγ

Mechanism of ubiquitous expression of mouse uncoupling protein 2 mRNA: control by cis-acting DNA element in 5′-flanking region

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