Differential regulation of trkA and p75 in noradrenergic pelvic autonomic ganglion cells after deafferentation of their cholinergic neighbours

Keast, Janet R.; Kepper, Mark E.

doi:10.1046/j.1460-9568.2001.01374.x

Cited by 10 publications

(14 citation statements)

References 55 publications

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“…Adult rat noradrenergic pelvic ganglion neurons express the neurotrophin receptors, TrkA and p75 NTR (Keast and Kepper, 2001), as would be expected for NGF-sensitive neurons (Huang and Reichart, 2001). In addition, it has been observed that NGF, BDNF and TrkB were expressed in major pelvic ganglion neurons and their expression increased after cyclophosphamide-induced cystitis in rats (Murray et al 2004).…”

confidence: 73%

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Effects of castration on the immunoreactivity to NGF, BDNF and their receptors in the pelvic ganglia of the male rat

Squillacioti¹,

Luca²,

Paino³

et al. 2009

Eur J Histochem

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Nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) and are members of the neurotrophin family, a family of neurotrophic factors that also includes neurotrophin (NT) 3 and NT4/5. Neurotrophins have essential roles in the survival, development and differentiation of neurons in the central and peripheral nervous systems. Neurotrophins exert their effects by binding to corresponding receptors which are formed by the tyrosine protein kinases TrkA, TrkB and TrkC, and the low affinity neurotrophic receptor (p75 NTR ). In the present study, using immunohistochemistry and quantitative analysis, we have investigated immunoreactivity to BDNF, NGF, TrkB, p75 NTR and TrkA in the pelvic ganglia of normal and castrated rats. Neurons of the pelvic ganglia expressed both these neurotrophins and their receptors. After castration the immunoreactivity persisted. However, the number of BDNF-and p75 NTR -IR cells statistically significant decreased after castration. These results suggest that castration modulates the expression of neurotrophins and their receptors in pelvic autonomic neurons.

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confidence: 73%

“…NGFand BDNF-IRs have been previously found by using ELISA in the MPG ganglia of male rats (Murray et al 2004). Moreover, TrkA-, TrkB-and p75 NTR -IRs have been found by using immunohistochemistry in the majority of neurons of these ganglia (Keast and Kepper, 2001;Murray et al 2004).…”

Section: Discussionmentioning

confidence: 97%

Effects of castration on the immunoreactivity to NGF, BDNF and their receptors in the pelvic ganglia of the male rat

Squillacioti¹,

Luca²,

Paino³

et al. 2009

Eur J Histochem

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“…In the PNS, the antidepressant effects on neurotrophin levels are not well studied. Because the urogenital tract (and the vas deferens) is rich in NGF and neurotrophin receptors (Keast, Kepper, 2001), the effect of fluoxetine treatment could be mediated by the synthesis of NGF. Of note, the fluoxetine effects were obtained only in denervated vas deferens.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the fact that antidepressants increase neurotrophin levels in the CNS, it is speculated that this process could also occur in the peripheral nervous system (PNS), where neuronal regeneration after a lesion also depends on neurotrophic factors, mainly NGF. In the urogenital tract, which is rich in neurotrophin receptors (Keast, Kepper, 2001), chronic treatment with exogenous NGF facilitates autonomic nerve regrowth (Burgers et al, 1991) and induces an increase neuronal activity (Milner et al, 1995). The vas deferens is a urogenital organ with a high neuronal network density, formed in great part by sympathetic nerves (Batra, 1974;Wanigasekara, Kepper, Keast, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of long-term fluoxetine treatment on adrenergic plasticity in rat vas deferens

Souza

Garcez-do-Carmo

Conceição

et al. 2014

Braz. J. Pharm. Sci.

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Chronic antidepressant administration increases neurotrophin levels in the central and peripheral nervous system, leading to an increase of neuronal sprouting, reestablishment of neural networks and neurotransmitter levels. Injured peripheral nerves regenerate at very slow rates. However, the recovery of the hypogastric nerve in rodents after injury is significantly improved with neurotrophin administration. Accordingly, our goal was to determine whether treatment with the antidepressant fluoxetine affects catecholamine levels and neuronal function, after surgical denervation of the rat vas deferens. Noradrenaline levels in the denervated vas deferens were higher in fluoxetine-treated animals than in the vehicle-treated group, as measured by high performance liquid chromatography. In functional studies of smooth muscle contraction, the responses induced by phenylephrine or ATP, as well as pre-synaptic α 2 -adrenoceptor reactivity, were not modified by chronic treatment with the antidepressant. However, the contraction mediated by neuronal release of noradrenaline induced by tyramine was increased on days 7 and 21 after denervation in rats treated with fluoxetine. These data indicate that fluoxetine can improve functional recovery after rat vas deferens denervation.Uniterms: Fluoxetine/chronic administration/effects. Denervation. Antidepressants/long term use/ experimental study. Adrenergic neurotransmission. Noradrenaline/neuronal release.A administração crônica de antidepressivos aumenta os níveis de neurotrofinas no sistema nervoso central, levando a um aumento da arborização neuronal, restabelecendo a rede neural e os níveis de neurotransmissores. Lesões do sistema nervoso periférico mostram uma regeneração muito lenta. Entretanto, a recuperação após a lesão do nervo hipogástrico em roedores é significativamente melhorada após a administração de neurotrofinas. Nesse sentido, nosso objetivo foi verificar se o tratamento com o antidepressivo, fluoxetina, interfere nos níveis de catecolaminas e na função neuronal, após a desnervação cirúrgica do ducto deferente de rato. Nos vasos deferentes desnervados, os níveis de catecolaminas nos grupos tratados com fluoxetina foram maiores que no grupo veículo, quantificados em cromatografia líquida de alta eficiência (CLAE). Nos estudos funcionais, a contração da musculatura lisa induzida pela fenilefrina ou pelo ATP, assim como a reatividade pré-sináptica α 2 -adrenérgica, não foram modificadas com o tratamento crônico de fluoxetina. Contudo, nas contrações mediadas pela liberação neuronal de norepinefrina induzida por tiramina, observou-se aumento da contração nos dias 7 e 21 após a desnevação em ratos tratados com fluoxetina. Esses dados indicam que a fluoxetina pode melhorar a recuperação funcional do vaso deferente de rato após a desnervação.

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“…Testosterone can have a negative effect on NGF. All rat pelvic noradrenergic neurons express the NGF receptors trkA and p75 [49]. NGF induces neurite growth in these neurons.…”

Section: Endocrine Abnormalitiesmentioning

confidence: 99%

Proposed New Pathophysiology of Chronic Prostatitis/Chronic Pelvic Pain Syndrome

Cho

Min

2015

Urogenit Tract Infect

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The most common type of prostatitis is category III, also known as chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). The current National Institutes of Health definition of CP/CPPS includes genitourinary pain with or without voiding symptoms in the absence of uropathogenic bacteria, as detected by standard microbiological methods, or other identifiable causes such as malignancy. Many different etiologies and mechanisms of pathogenesis of CP/CPPS have been proposed with a suggested role for immunological, neurological, endocrine, and psychological factors. We examined the data supporting the role of each of these areas and also examined the possible interrelationship of these factors in producing the symptoms of CP/CPPS. Prostatitis types IIIa and IIIb are classified according to the presence of pain without concurrent presence of bacteria; however, it is becoming more evident that, although levels of bacteria are not directly associated with levels of pain, the presence of bacteria might act as the initiating factor that drives primary activation of mast-cell-mediated inflammation in the prostate. The gate control theory provides a neurologic basis for the influence of both somatic and psychological factors on pain. Acceptance of chronic pain as a diagnosis may be difficult for the clinician and patient, however it is an important concept in the care of CP/CPPS, which enables the use of pain-directed therapies. Management of CP/CPPS will remain challenging; however, this review provides a better understanding of the condition and improved management strategies based on the newest evidence and concepts available. Keywords: Prostatitis; Chronic pain; Physiopathology INTRODUCTIONChronic prostatitis/chronic pelvic pain syndrome (CP/ CPPS) is a condition of chronic pelvic pain in men [1]. It has been estimated that between 2% and 14% of men worldwide may have symptoms of CP/CPPS [2,3]. The diagnosis is made on the basis of symptoms of pain with or without voiding symptoms in the absence of other identifiable causes [4]. The etiology of symptoms in a given patient can be from many causes. Whether there are identifiable, repeatable patterns of causes of symptoms, or just individual patients with distinct individual phenotypes are unknown. Because CP/CPPS is a common condition associated with significant physical, mental, and social burden, the medical community has made tremendous efforts to understand the condition and improve management strategies. Despite these efforts, answers remain elusive. Robust tools to measure symptom severity Our improved understanding of the etiology and clinical phenotypes associated with CP/CPPS has made the condition less of a clinical enigma. Acceptance of clinical definitions (and classification system) and validated, reliable outcome questionnaire (the CP symptom index) has led to high-quality treatment evidenced from randomized placebo-controlled trials. This article is a very timely review of the topic and provides the clinician with an improved understanding of CP/CPPS a...

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Differential regulation of trkA and p75 in noradrenergic pelvic autonomic ganglion cells after deafferentation of their cholinergic neighbours

Cited by 10 publications

References 55 publications

Effects of castration on the immunoreactivity to NGF, BDNF and their receptors in the pelvic ganglia of the male rat

Effects of castration on the immunoreactivity to NGF, BDNF and their receptors in the pelvic ganglia of the male rat

Effects of long-term fluoxetine treatment on adrenergic plasticity in rat vas deferens

Proposed New Pathophysiology of Chronic Prostatitis/Chronic Pelvic Pain Syndrome

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