Differential Regulation of the Expression of Nerve Growth Factor, Brain-Derived Neurotrophic Factor, and Neurotrophin-3 after Excitotoxicity in a Rat Model of Huntington's Disease

Canals, Josep M.; Marco, Sonia; Checa, Núria; Michels, Alice; Pérez‐Navarro, Esther; Arenas, Ernest; Alberch, Jordi

doi:10.1006/nbdi.1998.0211

Cited by 42 publications

(34 citation statements)

References 54 publications

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“…In our transgenic model we detected increases in NT3 levels in SN but not in LC that could also act on TrkB receptors. This may be explained that it could be a differential regulation between the ligand and its receptor as it has been described in other brain areas (Canals et al, 1998(Canals et al, , 1999. Although we cannot rule out that the lack of a fine dissection of the LC did not allow detecting small changes in NT-3 levels.…”

Section: Discussionmentioning

confidence: 79%

Transgenic mice overexpressing the full-length neurotrophin receptor TrkC exhibit increased catecholaminergic neuron density in specific brain areas and increased anxiety-like behavior and panic reaction

Dierssen

Gratacòs

Sahún

et al. 2006

Neurobiology of Disease

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Section: Discussionmentioning

confidence: 79%

Transgenic mice overexpressing the full-length neurotrophin receptor TrkC exhibit increased catecholaminergic neuron density in specific brain areas and increased anxiety-like behavior and panic reaction

Dierssen

Gratacòs

Sahún

et al. 2006

Neurobiology of Disease

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“…Total RNA was isolated from 0.5-g samples of frozen striatal tissue (3)(4)(5)(6) brains from each experimental condition). RNA extraction was carried out using the TRIzol reagent method modified for cerebral tissue using the Total RNA Isolation Reagent (Invitrogen Life Technologies Inc., Carlsbad, CA) and precipitation with ethanol.…”

Section: Rna Extraction and Rt-pcrmentioning

confidence: 99%

“…There are difficulties in assessing the expression of neurotrophins in the nervous system; for example, in early reports, brain-derived neurotrophic factor (BDNF) mRNA was not detected in striatal tissue by in situ hybridization [3] , but soon after, nerve growth factor (NGF), BDNF, neurotrophin-3 (NT-3) [4] and NT-4/5 [5] mRNA expression was described using the RNAse protection assay, and in most of these experiments glyceraldehyde dehydrogenase (GAPDH) was selected as the control gene.…”

Section: Introductionmentioning

confidence: 99%

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3-Nitropropionic acid modifies neurotrophin mRNA expression in the mouse striatum: 18S-rRNA is a reliable control gene for studies of the striatum

2012

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Objective The aim of the present study was to determine the changes in the mRNA levels of neurotrophins and their receptors in the striatal tissue of mice treated with 3-nitropropionic acid (3-NP). Methods At 1 and 48 h after the last drug administration, the mRNA expression of nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4/5 as well as their receptors p75, TrkA, TrkB and TrkC, was evaluated using semi-quantitative (semi-Q) and real-time RT-PCR. β-actin mRNA and ribosomal 18S (18S rRNA) were tested as internal controls. Results 3-NP treatment did not affect mRNA expression of all neurotrophins and their respective receptors equally. Also, differences in neurotrophin and receptor mRNA expression were observed between semi-Q and real-time RT-PCR. Real-time RT-PCR was more accurate in evaluating the mRNA expression of the neurotrophins than semi-Q, and 18S rRNA was more reliable than β-actin as an internal control. Conclusion Neurotrophins and their receptors expression is differentially affected by neuronal damage produced by inhibition of mitochondrial respiration with 3-NP treatment in low, sub-chronic doses in vivo.

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“…In contrast, NT-3 slightly induced BRINP1-mRNA in cultured hippocampal neurons. In this context, it is interesting to note that NT-3-mRNA has been reported to be down-regulated by various neural activities, including administration of glutamate in vivo [12].…”

Section: Regulation Of Brinp Family Mrna In Cultured Hippocampal Neuronsmentioning

confidence: 99%

Activity-dependent regulation of BRINP family genes

Motomiya

Kobayashi

Iwasaki

et al. 2007

Biochemical and Biophysical Research Communications

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We previously identified a family of novel developmentally regulated genes: BRINP1, 2, and 3, which are predominantly and widely expressed in the CNS from earlier developmental stages to adulthood. In the present study, we investigated the activity-dependent regulation of BRINP expression in the CNS. Among the three BRINP genes, BRINP1-mRNA was specifically up-regulated in the dentate gyrus of mouse hippocampus by kainic acid treatment.In cultured hippocampal neurons, the induction of BRINP1-mRNA was also observed by the activation of glutamate receptors. Although BDNF-mRNA is up-regulated in a similar activity-dependent manner, BDNF itself did not induce BRINP1-mRNA. From these results, the physiological roles of the activity-dependent induction of BRINP1-mRNA are discussed.

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Differential Regulation of the Expression of Nerve Growth Factor, Brain-Derived Neurotrophic Factor, and Neurotrophin-3 after Excitotoxicity in a Rat Model of Huntington's Disease

Cited by 42 publications

References 54 publications

Transgenic mice overexpressing the full-length neurotrophin receptor TrkC exhibit increased catecholaminergic neuron density in specific brain areas and increased anxiety-like behavior and panic reaction

Transgenic mice overexpressing the full-length neurotrophin receptor TrkC exhibit increased catecholaminergic neuron density in specific brain areas and increased anxiety-like behavior and panic reaction

3-Nitropropionic acid modifies neurotrophin mRNA expression in the mouse striatum: 18S-rRNA is a reliable control gene for studies of the striatum

Activity-dependent regulation of BRINP family genes

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