Differential regulation of the durum wheat Pathogenesis-related protein (PR1) by Calmodulin TdCaM1.3 protein

Ghorbel, Mohamed Habib; Zribi, Ikram; Missaoui, Khawla; Drira-Fakhfekh, Marwa; Azzouzi, Basma; Brini, Faïçal

doi:10.1007/s11033-020-06053-7

Cited by 32 publications

(39 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, TdPR1.2 showed an antibacterial activity against some Gram + and Gram − bacteria and against the fungi Septoria tritici. Moreover, we provide experimental evidence that TdPR1.2 binds to calmodulins (CaMs) in Calcium dependent manner and that this binding stimulates the antimicrobial effect of TdPR1.2 in presence of both Ca 2+ and Mn 2+ cations [27]. In the present study, we show that TdPR1.2 protein represents a putative SCP_PR-1_like domain that belongs to SCP superfamily, conserved in other PR1 homologues.…”

Section: Introductionmentioning

confidence: 50%

“…(5'-GAATTCGGCTTCGGCGTCAAG-3'). The amplified products were cloned into the pET28a vector at the EcoRI site to express the pET28a-TdPR1.2 fusion protein, which has a poly-Histidine tag at the N-terminus [27]. The recombinant protein His_TdPR1.2 was produced in the Rosetta E. coli strain (DE3) (Novagen) after cloning of the corresponding ORF in-frame with a poly histidine tag into the pET28a expression vectors (Novagen, Madison).…”

Section: Tdpr12 Protein Expressionmentioning

confidence: 99%

“…For this, we used heterologous expression in E. coli cells. The full-length cDNA was cloned in the pET28a expression vector transformed into E. coli (BL21 strain) as previously described [27].…”

Section: Recombinants Tdpr12 Proteins Confers Contrasting Bacterial Tolerance To Ionic and Osmotic Stressmentioning

confidence: 99%

“…We have recently isolated and characterized a novel PR1.2 gene (TdPR1.2) from durum wheat [27]. TdPR1.2 gene was up-regulated in roots, stems and leaves after plant treatment with Salicylic Acid (SA).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to <i>E. coli</i> Transformant Cells under Stress Conditions

Ghorbel¹,

Zribi²,

Haddaji³

et al. 2021

AiM

Self Cite

View full text Add to dashboard Cite

The pathogenesis-related proteins 1 (PR-1) gene family play important roles in the plant metabolism in response to biotic and abiotic stresses. The wheat TdPR1.2 has been previously isolated and characterized. Here we showed by bio-informatic analysis that TdPR1.2 contains six cysteine residues that are conserved between all PR-1 proteins tested. Using ScanProsite tool, we found that TdPR1.2 structure has a CRISP family signature 1 and 2 located at the C-terminal part of the protein. Those two domains are conserved in many identified PR1.2 proteins in plants. Moreover, SignalIP-5.0 analysis revealed that TdPR1.2 contains a putative signal peptide formed by 25 amino acids at the N-terminal extremity. The presence of this signal peptide suggested that the mature proteins will be secreted after the cleavage of the signal sequence. Further, we investigate the role of the TdPR1.2 proteins in the growth of Escherichia coli transformants cells under different abiotic stresses. Our results showed that the full-length form of TdPR1.2 enhanced tolerance of E. coli against salt and osmotic stress but not to KCl. Moreover, TdPR1.2 protein confers bacterial tolerance to heavy metals in solid and liquid mediums. Based on these results, we suggest that the TdPR1.2 protein could play an important role in response to abiotic stress conditions.

show abstract

Section: Introductionmentioning

confidence: 50%

Section: Tdpr12 Protein Expressionmentioning

confidence: 99%

Section: Recombinants Tdpr12 Proteins Confers Contrasting Bacterial Tolerance To Ionic and Osmotic Stressmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to <i>E. coli</i> Transformant Cells under Stress Conditions

Ghorbel¹,

Zribi²,

Haddaji³

et al. 2021

AiM

Self Cite

View full text Add to dashboard Cite

show abstract

“…30,31 Therefore, we evaluated the anti-bacterial activity of purified MePR1 protein (Figure S1A-B) against Xam by the radial diffusion assay. 39 From the diameter of the anti-bacterial zone, the MePR1 had a significant inhibitory effect on the growth of Xam. With the increase of protein quantities, the anti-bacterial activity was more powerful.…”

Section: Measmt2 Promotes the Antibacterial Activity Of Mepr1 Protein...mentioning

confidence: 99%

Fine‐tuning of pathogenesis‐related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight

Guo

Bai

Wei

et al. 2021

Journal of Pineal Research

View full text Add to dashboard Cite

Melatonin is widely involved in plant disease resistance through modulation of immune responses. Pathogenesis-related (PR) proteins play important roles in plant immune responses. However, the direct association between melatonin biosynthetic enzyme and PR protein remains elusive in plants. In this study, we found that N-acetylserotonin O-methyltransferase 2 (MeASMT2) physically interacted with MePR1 in vitro and in vivo, thereby promoting the anti-bacterial activity of MePR1 against Xanthomonas axonopodis pv. manihotis (Xam). Consistently, MeASMT2 improved the effect of MePR1 on positively regulating cassava disease resistance. In addition, we found that type 2C protein phosphatase 1 (MePP2C1) interacted with MeASMT2 to interfere with MePR1-MeASMT2 interaction, so as to inhibiting the effect of MeASMT2 and MePR1 on positively regulating cassava disease resistance. In contrast to the increased transcripts of MeASMT2 and MePR1 in response to Xam infection, the transcript of MePP2C1 was decreased upon Xam infection. Therefore, disease activated MeASMT2 was released from disease inhibited MePP2C1, so as to improving the anti-bacterial activity of MePR1, resulting in improved immune response. In summary, this study illustrates the dynamic modulation of the MePP2C1-MeASMT2-MePR1 module on cassava defense response against cassava bacterial blight (CBB), extending the understanding of the correlation between melatonin biosynthetic enzyme and PR in plants. K E Y W O R D Scassava bacterial blight, melatonin biosynthetic enzyme, pathogenesis-related protein, proteinprotein interaction, type 2C protein phosphatase

show abstract

The wheat pathogenesis-related protein (TdPR1.2) enhanced tolerance to abiotic and biotic stresses in transgenic Arabidopsis plants

Zribi,

Ghorbel,

Jrad

et al. 2024

Protoplasma

View full text Add to dashboard Cite

Differential regulation of the durum wheat Pathogenesis-related protein (PR1) by Calmodulin TdCaM1.3 protein

Cited by 32 publications

References 49 publications

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to <i>E. coli</i> Transformant Cells under Stress Conditions

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to <i>E. coli</i> Transformant Cells under Stress Conditions

Fine‐tuning of pathogenesis‐related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight

The wheat pathogenesis-related protein (TdPR1.2) enhanced tolerance to abiotic and biotic stresses in transgenic Arabidopsis plants

Contact Info

Product

Resources

About

Differential regulation of the durum wheat Pathogenesis-related protein (PR1) by Calmodulin TdCaM1.3 protein

Cited by 32 publications

References 49 publications

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to &lt;i&gt;E. coli&lt;/i&gt; Transformant Cells under Stress Conditions

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to &lt;i&gt;E. coli&lt;/i&gt; Transformant Cells under Stress Conditions

Fine‐tuning of pathogenesis‐related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight

The wheat pathogenesis-related protein (TdPR1.2) enhanced tolerance to abiotic and biotic stresses in transgenic Arabidopsis plants

Contact Info

Product

Resources

About

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to <i>E. coli</i> Transformant Cells under Stress Conditions

The Wheat Pathogenesis Related Protein (TdPR1.2) Ensures Contrasting Behaviors to <i>E. coli</i> Transformant Cells under Stress Conditions