Differential Regulation of Th1-Type and Th2-Type Cytokine Profiles in Pancreatic Islets of C57BL/6 and BALB/c Mice by Multiple Low Doses of Streptozotocin

Müller, Andreas; Schott-Ohly, Patricia; Dohle, Claudia; Gleichmann, Helga

doi:10.1078/0171-2985-00109

Cited by 87 publications

(72 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The beta-cell injury induced by consecutive low dose injections of STZ, as performed in this study, involves infiltration of islets by activated macrophages and some lymphocytes. [47][48][49][50] This is associated with increased local and systemic release of interferon-g and tumor necrosis factor-a. It is apparent that islet cell injury and death is dependent on both the initial toxic effect of low-dose STZ, and the subsequent inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

In vivo expression of GLP-1/IgG-Fc fusion protein enhances beta-cell mass and protects against streptozotocin-induced diabetes

et al. 2007

View full text Add to dashboard Cite

Glucagon-like peptide 1 (GLP-1) and its analogue exendin-4 (Ex4) have displayed potent glucose homeostasis-modulating characteristics in type 2 diabetes (T2D). However, there are few reports of effectiveness in type 1 diabetes (T1D) therapy, where there is massive loss of b cells. We previously described a novel GLP-1 analogue consisting of the fusion of active GLP-1 and IgG heavy chain constant regions (GLP-1/IgG-Fc), and showed that in vivo expression of the protein, via electroporation-enhanced intramuscular plasmid-based gene transfer, normalized blood glucose levels in T2D-prone db/db mice. In the present study, GLP-1/IgG-Fc and Ex4/IgG-Fc were independently tested in multiple low-dose streptozotocin-induced T1D. Both GLP-1/ IgG-Fc and Ex4/IgG-Fc effectively reduced fed blood glucose levels in treated mice and ameliorated diabetes symptoms, where as control IgG-Fc had no effect. Treatment with GLP-1/IgG-Fc or Ex4/IgG-Fc improved glucose tolerance and increased circulating insulin and GLP-1 levels. It also significantly enhanced islet beta-cell mass, which is likely a major factor in the amelioration of diabetes. This suggests that GLP-1/IgG-Fc gene therapy may be applicable to diseases where there is either acute or chronic beta-cell injury.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo expression of GLP-1/IgG-Fc fusion protein enhances beta-cell mass and protects against streptozotocin-induced diabetes

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The administration of streptozotocin (STZ) to mice [33][34][35] and rats 36 represents a good model of diabetes development for several reasons: (i) islet lesions in this experimental model resemble those of human insulitis, 35 (ii) the animals used are normal and do not have underlying immune abnormalities like BB rat 37 and male macrosomic offspring for the study. Indeed, sex hormones have been associated with the prevalence, the susceptibility and the severity of autoimmune diseases.…”

Section: Discussionmentioning

confidence: 99%

N-3 Fatty acids modulate antioxidant status in diabetic rats and their macrosomic offspring

et al. 2006

View full text Add to dashboard Cite

Objective: We investigated the role of dietary n-3 polyunsaturated fatty acids (n-3 PUFA) in the modulation of total antioxidant status in streptozotocin (STZ)-induced diabetic rats and their macrosomic offspring. Design: Female wistar rats, fed on control diet or n-3 PUFA diet, were rendered diabetic by administration of five mild doses of STZ on day 5 and were killed on days 12 and 21 of gestation. The macrosomic (MAC) pups were killed at the age of 60 and 90 days. Measurements: Lipid peroxidation was measured as the concentrations of plasma thiobarbituric acid reactive substances (TBARS), and the total antioxidant status was determined by measuring (i) plasma oxygen radical absorbance capacity (ORAC), (ii) plasma vitamin A, E and C concentrations, and (iii) antioxidant enzymes activities in erythrocytes. The plasma lipid concentrations and fatty acid composition were also determined. Results: Diabetes increased plasma triglyceride and cholesterol concentrations, whereas macrosomia was associated with enhanced plasma cholesterol and triglyceride levels, which diminished by feeding n-3 PUFA diet. N-3 PUFA diet also reduced increased plasma TBARS and corrected the decreased ORAC values in diabetic rats and their macrosomic offspring. EPAX diet increased the diminished vitamin A levels in diabetic mothers and vitamin C concentrations in macrosomic pups. Also, this diet improved the decreased erythrocyte superoxide dismutase and glutathione peroxidase activities in diabetic and macrosomic animals. Conclusion: Diabetes and macrosomia were associated with altered lipid metabolism, antioxidant enzyme activities and vitamin concentrations. N-3 PUFA diet improved hyperlipidemia and restored antioxidant status in diabetic dams and MAC offspring.

show abstract

“…Dans cet article, nous ne décrirons pas le rôle des cellules Th1 dans la pathogenèse du diabète de type 1 car il est bien connu que ces cellules, après l'infiltration du pancréas, détrui-sent les cellules bêta et aggravent cette pathologie [4,5]. Nous avons récemment développé un modèle de bébés macrosomiques, nés de mères diabé-tiques, qui à l'age adulte deviennent obèses, marqués par l'augmentation de taux de lipides (cholestérol total, LDL, VLDL, etc.)…”

Section: Cellules Th1 Et Th2 Dans L'obésité/l'insulino-résistanceunclassified

Inflammation et immunité : implications dans l’obésité et le diabète de type 2

Khan

2006

OCL

View full text Add to dashboard Cite

show abstract

Differential Regulation of Th1-Type and Th2-Type Cytokine Profiles in Pancreatic Islets of C57BL/6 and BALB/c Mice by Multiple Low Doses of Streptozotocin

Cited by 87 publications

References 45 publications

In vivo expression of GLP-1/IgG-Fc fusion protein enhances beta-cell mass and protects against streptozotocin-induced diabetes

In vivo expression of GLP-1/IgG-Fc fusion protein enhances beta-cell mass and protects against streptozotocin-induced diabetes

N-3 Fatty acids modulate antioxidant status in diabetic rats and their macrosomic offspring

Inflammation et immunité : implications dans l’obésité et le diabète de type 2

Contact Info

Product

Resources

About