Differential regulation of proteoglycan 4 by IL-1alpha and TGF-beta1 in rat condylar chondrocytes

Cheng, Jie; Wang, Y.; Wu, Yanhu

doi:10.1016/j.ijom.2011.07.669

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies in fibroblasts suggest that CTGF is a down-stream mediator of TGFb1-induced collagen synthesis (Grotendorst et al 1996;Duncan et al 1999). It was previously reported that PRG4 expression is positively regulated by exogenously added TGF-beta1 and negatively regulated by IL-1beta in cultured cells (Lee et al 2008;Cheng et al 2010). Therefore, we hypothesized that decreased PRG4 mRNA and protein expression detected in our study in tendons of old rats could be mediated by the decrease in TGFb1 and CTGF expression.…”

Section: Discussionmentioning

confidence: 62%

Age-related changes in structure and extracellular matrix protein expression levels in rat tendons

Kostrominova

Brooks

2013

AGE

View full text Add to dashboard Cite

The musculoskeletal system (muscle-tendonbone) demonstrates numerous age-related changes, with modifications in tendons the least well studied, although increased predisposition to tendinopathy and rupture have been reported. In order to gain insights into the basis of age-associated increase in tendon injuries, we compared Achilles and tibialis anterior tendons and myotendinous junctions (MTJs) from 3-to 5-and 22-to 25-month-old rats for underlying structure and composition. Significant decreases were observed by qRT-PCR for collagen I, III, and V mRNA expression in tendons of old rats, but immunostaining detected no apparent differences in collagen I and V expression on the protein level. Tendons of old compared with young rats had decreased mRNA expression levels of proteoglycan 4 (PRG4) and elastin (Eln), but no differences in the mRNA expression of connective tissue growth factor, TGF-beta 1, or stromal cell-derived factor 1. For PRG4, immunostaining showed good correlation with qRT-PCR results. This is the first study to show reductions in PRG4 in tendons and MTJs of old rats. Decreased PRG4 expression in tendons could result in increased tendon stiffness and may be associated with decreased activity in the elderly. The diminished collagen mRNA expression in combination with decreased PRG4 and Eln mRNA expression may be associated with increased risk of tendon injury with aging.

show abstract

Section: Discussionmentioning

confidence: 62%

Age-related changes in structure and extracellular matrix protein expression levels in rat tendons

Kostrominova

Brooks

2013

AGE

View full text Add to dashboard Cite

show abstract

“…TGF-b's have been shown to stimulate PRG4 production in chondrocytes, 34,[41][42][43] as well as infrapateller fat pad progenitor cells. 44 MSCs in alginate culture secrete PRG4, 19 however factors which stimulate or regulate this production are unknown.…”

Section: Discussionmentioning

confidence: 99%

Matrix molecule influence on chondrocyte phenotype and proteoglycan 4 expression by alginate‐embedded zonal chondrocytes and mesenchymal stem cells

Coates

Riggin

Fisher

2012

Journal Orthopaedic Research

View full text Add to dashboard Cite

Articular cartilage resists load and provides frictionless movement at joint surfaces. The tissue is organized into the superficial, middle, deep, and calcified zones throughout its depth, each which serve distinct functions. Proteoglycan 4 (PRG4), found in the superficial zone, is a critical component of the joint's lubricating mechanisms. Maintenance of both the chondrocyte and zonal chondrocyte phenotype remain challenges for in vitro culture and tissue engineering. Here we investigate the expression of PRG4 mRNA and protein by primary bovine superficial zone chondrocytes, middle/deep zone chondrocytes, and mesenchymal stem cells encapsulated in alginate hydrogels with hyaluronic acid (HA) and chondroitin sulfate (CS) additives. Chondrogenic phenotype and differentiation markers are evaluated by mRNA expression, histochemical, and immunohistochemical staining. Results show middle/ deep cells express no measurable PRG4 mRNA by day 7. In contrast, superficial zone cells express elevated PRG4 mRNA throughout culture time. This expression can be significantly enhanced up to 15-fold by addition of both HA and CS to scaffolds. Conversely, PRG4 mRNA expression is downregulated (up to 5-fold) by CS and HA in differentiating MSCs, possibly due to build up of entrapped protein.HA and CS demonstrate favorable effects on chondrogenesis by upregulating transcription factor Sox9 mRNA (up to 4.6-fold) and downregulating type I collagen mRNA (up to 18-fold). Results highlight the important relationship between matrix components and expression of critical lubricating proteins in an engineered cartilage scaffold.

show abstract

“…It is non-invasive and results in rapid patient response. Earlier studies showed that during the occurrence and development of TMD, cytokines such as IL-1, IL-6, and TNF-a are involved in inflammation of the synovium, leading to destruction of articular cartilage and excessive apoptosis of chondrocytes in the soft tissue of bone and joints as a result of increased NO content and the imbalance of local metabolism in the joint [23][24][25]. ESW can reduce NO content in the articular fluid, reduce the apoptosis of chondrocytes, promote the proliferation of articular cartilage and the repair of cartilage defects, and inhibit the secretion of inflammatory cytokines [26,27].…”

Section: Discussionmentioning

confidence: 99%

Treatment of Temporomandibular Joint Disorders by Ultrashort Wave and Extracorporeal Shock Wave: A Comparative Study

2020

Med Sci Monit

View full text Add to dashboard Cite

Departmental sources Background:This study was carried out to compare the therapeutic efficacy of extracorporeal shock wave (ESW) and ultrashort wave (UW) for temporomandibular joint disorder (TMD). Material/Methods:A total of 80 patients with myofascial pain and TMD were enrolled in this study. The subjects were randomized to receive ESW or UW treatments. Patients in the ESW group received 1 ESW treatment for 4 weeks and patients in the US group were given US treatment once a day for 5 days per week for 4 weeks. The pain was measured using visual analog scale (VAS) and mouth opening was determined as pain-free maximum mouth opening (MMO) before and 4 weeks after the treatments. Other parameters assessed included functional indexes of temporomandibular joint such as mandibular movement (MM), joint noise (JN), joint press (JP), and disability index (DI). Results:After therapy, VAS, MMO, MM, JN, JP, and DI in ESW group, and VAS in UW group were significantly improved (P<0.05) as compared to before therapy. VAS, MMO, and the functional indexes of temporomandibular joint in the ESW group were significantly better than those in the UW group (1.79 vs. 2.00, 3.23 vs. 2.03, 1.79 vs. 2.41, 1.45 vs. 2.27, 1.55 vs. 2.59, and 3.30 vs. 4.79, respectively. P<0.05). Conclusions:ESW significantly reduces pain and improves the functional indexes of temporomandibular joint and mouth opening limit for TMD patients as compared with UW therapy.

show abstract

Differential regulation of proteoglycan 4 by IL-1alpha and TGF-beta1 in rat condylar chondrocytes

Cited by 3 publications

References 0 publications

Age-related changes in structure and extracellular matrix protein expression levels in rat tendons

Age-related changes in structure and extracellular matrix protein expression levels in rat tendons

Matrix molecule influence on chondrocyte phenotype and proteoglycan 4 expression by alginate‐embedded zonal chondrocytes and mesenchymal stem cells

Treatment of Temporomandibular Joint Disorders by Ultrashort Wave and Extracorporeal Shock Wave: A Comparative Study

Contact Info

Product

Resources

About