Differential regulation of components of the apoptotic and ubiquitin-mediated proteolytic pathway in slow- and fast- twitch muscle type in lambs receiving increasing amounts of dietary non-structural carbohydrate

Greenwood, S.L.; Steele, Marcella; AlZahal, Ousama; Hook, Sarah E.; Swanson, K. C.; McBride, B.W.

doi:10.4141/cjas10006

Cited by 1 publication

(2 citation statements)

References 27 publications

(29 reference statements)

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“…The lambs were fed either a control diet (CON) consisting of 71.6% dehydrated alfalfa pellet and 28.4% grain pellet (20% corn, 40% wheat, 40% barley pellet; Floradale Feed Mill Ltd., Guelph, ON) or transitioned to a HG diet comprising 20.9% dehydrated alfalfa pellet and 79.1% grain pellet. Both diets were fed at 2.5% of bodyweight [dry matter (DM) basis] and complete chemical composition of the diets has been previously reported by Greenwood et al (2010). In summary, the neutral detergent fibre percentage was higher in the CON diet (38.6 vs. 20%) and the non-fibre carbohydrate percentage was higher in the HG diet (44.2 vs. 67.2%).…”

Section: Dietary Treatmentsmentioning

confidence: 95%

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An increase in dietary non-structural carbohydrates alters the structure and metabolism of the rumen epithelium in lambs

et al. 2012

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Steele, M. A., Greenwood, S. L., Croom, J. and McBride, B. W. 2012. An increase in dietary non-structural carbohydrates alters the structure and metabolism of the rumen epithelium in lambs. Can. J. Anim. Sci. 92: 123–130. This study investigated the effect of a grain challenge on the structure and metabolism of the rumen epithelium in lambs. In a randomized design, lambs (n = 8) received either a control diet [30% dry matter (DM) grain], or a diet of increasing amounts of grain to a maximum inclusion of 79% of DM for 12 d prior to slaughter. Rumen papillae were collected from the ventral sac on day 13 and prepared for histological and gene expression analyses. All lambs fed the high-grain diet were diagnosed with ruminal parakeratosis as the thickness of the corneum was higher (P<0.01) compared with control lambs (51.0±2.3 vs. 17.3±2.5 µm). Plasma beta-hydroxybutyric acid concentrations increased linearly (P<0.05) with increased grain consumption compared with the control. However, the relative mRNA expression of the ketogenic enzyme 3-hydroxy-3-methyl-glutaryl CoA-synthase (HMGCS2) in rumen papillae was not different between treatments. The expression of cholesterolgenic enzyme HMGCS1 was down-regulated by 0.70±0.06 (P<0.05) fold in lambs fed the high-grain diet compared with the control. These results suggest that a short-term grain challenge in lambs is associated with altered rumen epithelium metabolism and structural changes indicative of ruminal parakeratosis.

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Section: Dietary Treatmentsmentioning

confidence: 95%

“…An outline of the animal experiment and treatments has been previously described by Greenwood et al (2010). In brief, eight Canadian-Arcott wether lambs (9791.9 d of age; 31.792.9 kg body weight) were housed in individual pens at the Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.…”

Section: Methodsmentioning

confidence: 99%