Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury

Han, Ki-Hwan; Lee, U-Young; Jang, Yoon-Seong; Cho, Yoon Mi; Jang, Young Min; Hwang, Inah; Ghee, Jung Yeon; Lim, Sang-Hyun; Kim, Wan-Young; Yang, Chul Woo; Kim, Jin; Kwon, Oh-Joo

doi:10.1152/ajprenal.00009.2006

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…Increased expression of ENaC in the CRF rats is in agreement with the results of a previous study stating that remnant kidney models result in selective augmentation of the Na transport processes in the cortical collecting ducts [11]. Also, principal cells have been reported to be resistant to cell death in kidney injury [29]. This could be one reason why expression of ENaC-α remained continuously high over the entire period.…”

Section: Discussionsupporting

confidence: 90%

Changes in the Sodium and Potassium Transporters in the Course of Chronic Renal Failure

et al. 2010

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Background: In chronic renal failure (CRF), residual nephrons can increase their excretion of sodium (Na) and potassium (K). However, the mechanisms of renal Na and K regulation in late-stage CRF have not been clearly investigated. Methods: We examined altered expression of major renal Na and K transporters in Sprague-Dawley rats at 4 and 12 weeks after a 5/6 nephrectomy. Results: CRF rats were azotemic and had gradually increased levels of urinary Na and K excretion over time. At 4 weeks, the abundance of Na-K-2Cl cotransporter (NKCC2), and Na-Cl cotransporter (NCC) in CRF rats increased significantly (477 and 222% of the control, respectively). In contrast, expression of NKCC2 and NCC decreased markedly at 12 weeks (55.4 and 30.8%, respectively). Expression of epithelial Na channel-α increased throughout the whole period. The abundance of renal outer medullary K-channel (ROMK) and Na-K-ATPase did not decrease at 4 weeks, but it was reduced at 12 weeks. Conclusion: We suggest that increased urinary Na excretion in late-stage CRF may be associated with decreased expression of renal Na transporters except ENaC compared to early-stage CRF, and that increased urinary K excretion in the late stage of CRF may not be related to expression of ROMK.

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Section: Discussionsupporting

confidence: 90%

Changes in the Sodium and Potassium Transporters in the Course of Chronic Renal Failure

et al. 2010

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“…A possible motif upstream of the C2A domain is indicated. The protein product of Syt17 has been referred to as B/K protein, but not much is known about its function [85]. …”

Section: Resultsmentioning

confidence: 99%

A manual collection of Syt, Esyt, Rph3a, Rph3al, Doc2, and Dblc2 genes from 46 metazoan genomes - an open access resource for neuroscience and evolutionary biology

Craxton

2010

BMC Genomics

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BackgroundSynaptotagmin proteins were first identified in nervous tissue, residing in synaptic vesicles. Synaptotagmins were subsequently found to form a large family, some members of which play important roles in calcium triggered exocytic events. These members have been investigated intensively, but other family members are not well understood, making it difficult to grasp the meaning of family membership in functional terms. Further difficulty arises as families are defined quite legitimately in different ways: by common descent or by common possession of distinguishing features. One definition does not necessarily imply the other. The evolutionary range of genome sequences now available, can shed more light on synaptotagmin gene phylogeny and clarify family relationships. The aim of compiling this open access collection of synaptotagmin and synaptotagmin-like sequences, is that its use may lead to greater understanding of the biological function of these proteins in an evolutionary context.Results46 metazoan genomes were examined and their complement of Syt, Esyt, Rph3a, Rph3al, Doc2 and Dblc2 genes identified. All of the sequences were compared, named, then examined in detail. Esyt genes were formerly named Fam62. The species in this collection are Trichoplax, Nematostella, Capitella, Helobdella, Lottia, Ciona, Strongylocentrotus, Branchiostoma, Ixodes, Daphnia, Acyrthosiphon, Tribolium, Nasonia, Apis, Anopheles, Drosophila, Caenorhabditis, Takifugu, Tetraodon, Gasterosteus, Oryzias, Danio, Xenopus, Anolis, Gallus, Taeniopygia,Ornithorhynchus, Monodelphis, Mus and Homo. All of the data described in this paper is available as additional files.ConclusionsOnly a subset of synaptotagmin proteins appear able to function as calcium triggers. Syt1, Syt7 and Syt9 are ancient conserved synaptotagmins of this type. Some animals carry extensive repertoires of synaptotagmin genes. Other animals of no less complexity, carry only a small repertoire. Current understanding does not explain why this is so. The biological roles of many synaptotagmins remain to be understood. This collection of genes offers prospects for fruitful speculation about the functional roles of the synaptotagmin repertoires of different animals and includes a great range of biological complexity. With reference to this gene collection, functional relationships among Syt, Esyt, Rph3a, Rph3al, Doc2 and Dblc2 genes, which encode similar proteins, can better be assessed in future.

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“…Tubular cell injury as well as diffuse endothelial cell damage has been demonstrated in I/R and both tubular and endothelial cells contribute to the infl ammatory process. Infl ammation has become recognized as a critical component of I/R injury (27,28).…”

Section: Discussionmentioning

confidence: 99%

Topiramate has protective effect on renal injury

Cüre¹,

Cüre²,

Tümkaya³

et al. 2015

BLL

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Abstract:Background: Topiramate (TPM) decreases tumor necrosis factor-alpha (TNF-α) and oxidative stress. We investigated protective effects of TPM on cell damage in kidney tissue during ischemia-reperfusion (I/R) damage. Methods: A total of 30 male Wistar albino rats were divided into three groups: control, I/R, and I/R plus TPM (I/ R+TPM). Laparotomy without I/R injury was performed in control group. After laparotomy, cross ligation of infrarenal abdominal aorta was applied for two hours in I/R groups which was followed by two hours of reperfusion. TPM (100 mg/kg/day) was orally administrated to animals in the I/R+TPM group for seven consecutive days before I/R. Results: The I/R group's TNF-α and interleukin-1 beta (IL-1β) levels were signifi cantly higher (1184.2 ± 129.1 pg/mg protein; 413.1 ± 28.8 pg/mg protein, respectively) than those of the control (907.8 ± 113.0 pg/mg protein, p = 0.002; 374.7 ± 23.7 pg/mg protein, p = 0.010, respectively) and I/R+TPM groups (999.5 ± 115.2 pg/ mg protein, p < 0.001; 377.9 ± 30.9 pg/mg protein, p = 0.007, respectively). Conclusion: TPM may partially prevent renal damage in rats. The opening of new horizons of this kind of knowledge will help understand the complex challenge in the prevention of renal I/R damage (Tab. 1, Fig. 3, Ref. 42). Text in PDF www.elis.sk.

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Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury

Abstract: Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury.

Cited by 7 publications

References 31 publications

Changes in the Sodium and Potassium Transporters in the Course of Chronic Renal Failure

Changes in the Sodium and Potassium Transporters in the Course of Chronic Renal Failure

A manual collection of Syt, Esyt, Rph3a, Rph3al, Doc2, and Dblc2 genes from 46 metazoan genomes - an open access resource for neuroscience and evolutionary biology

Topiramate has protective effect on renal injury

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