Differential regulation of angiotensin converting enzyme 2 and nuclear factor-κB by angiotensin II receptor subtypes in type 2 diabetic kidney

Diminazene aceturate prevents nephropathy by increasing glomerular ACE2 and AT2 receptor expression in a rat model of type1 diabetes

“…ACE2 expression was increased only in tubules and decreased in glomeruli, whereas mRNA expression was unaltered in these diabetic rats. Previously, we have shown increased expression of ACE2 in kidney sections from type 2 diabetic rats (Pandey et al ., ). These reports clearly indicate the site‐specific expression of ACE and ACE2 protein and of their mRNA, but their activities are not well correlated in diabetic kidney, and even species, strain, age, sex and the extent of glucose levels may also affect the expression of these two enzymes.…”

“…Immunohistochemistry was performed as described previously (Pandey et al, 2015). Briefly, kidney sections (5 μm) were taken from paraffin blocks and deparaffinized with xylene, followed by antigen retrieval by heating in citrate buffer (10 mmol·L À1 ).…”

Eugenol ameliorates renal damage in streptozotocin‐induced diabetic rats

“…In HE staining, glomerular damage was evaluated using a semi quantitative scoring method. HE stained sections were observed under 400X magnification with a digital microscope and scoring was done as follows: 0 = no lesion, 1 = <25% damage, 2 = 25–49 % damage, 3 = 50–74% damage, 4 = 75–100% damage, respectively …”

“…HE stained sections were observed under 400X magnification with a digital microscope and scoring was done as follows: 0 = no lesion, 1 = <25% damage, 2 = 25-49 % damage, 3 = 50-74% damage, 4 = 75-100% damage, respectively. [30] Sections stained with PAS stain were observed for stromal hyperplasia in the glomerular mesangial area and basement membrane thickening in the glomerular capillary plexus. PAS stained glomeruli were observed under 400X magnification on a digital microscope and mesangial matrix index was calculated.…”

The eye and the kidney: twin targets in diabetes

“…Data on ACE2 is available from different areas of India. Angiotensin 1 receptor and angiotensin 2 receptors regulate NF-kB and ACE2, and provide a potential for identifying drug targets [26]. ACE (I/D) gene polymorphisms were reported to predispose to DN from Kutch region in western India.…”