Differential regulation of agonist-stimulated Ca2+ influx in acini of rat pancreas and submandibular gland

Hurley, Thomas W.; Brinck, R. W.

doi:10.1016/0003-9969(92)90109-l

Cited by 6 publications

(2 citation statements)

References 17 publications

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“…Rat submaxillary gland has frequently been studied for pharmacological characterization of the MChRs involved in secretion by examining the following assays: 86 rubidium efflux ( Bovell et al ., 1989 ), calcium mobilization ( Hurley & Brinck, 1992 ; Valdez & Turner, 1991 ), inositol phosphates accumulation ( Laniyonu et al ., 1990 ; Abdallah et al ., 1992 ), and inhibition of adenylyl cyclase activity ( Dai et al ., 1991 ; Laniyonu et al ., 1990 ; Abdallah et al ., 1992 ). While these approaches offer some insight into cellular function in rat submaxillary gland, they generally lack a robust method to determine antagonist affinity and thus characterize the muscarinic subtype.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of rat submaxillary gland muscarinic receptors using microphysiometry

Meloy

Daniels

Hegde

et al. 2001

British J Pharmacology

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1 Muscarinic cholinoceptors (MChR) in freshly dispersed rat salivary gland (RSG) cells were characterized using microphysiometry to measure changes in acidi®cation rates. Several non-selective and selective muscarinic antagonists were used to elucidate the nature of the subtypes mediating the response to carbachol. 2 The eects of carbachol (pEC 50 =5.74+0.02 s.e.mean; n=53) were highly reproducible and most antagonists acted in a surmountable, reversible fashion. The following antagonist rank order, with apparent anity constants in parentheses, was noted: 4-DAMP (8.9)=atropine (8.9)4tolterodine (8.5)4oxybutynin (7.9)4S-secoverine (7.2)4pirenzepine (6.9)4himbacine (6.8)4AQ-RA 741 (6.6)4methoctramine (5.9). 3 These studies validate the use of primary isolated RSG cells in microphysiometry for pharmacological analysis. These data are consistent with, and extend, previous studies using alternative functional methods, which reported a lack of dierential receptor pharmacology between bladder and salivary gland tissue. 4 The antagonist anity pro®le signi®cantly correlated with the pro®le at human recombinant muscarinic M 3 and M 5 receptors. Given a lack of antagonists that discriminate between M 3 and M 5 , de®nitive conclusion of which subtype(s) is present within RSG cells cannot be determined.

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Section: Introductionmentioning

confidence: 99%

Functional characterization of rat submaxillary gland muscarinic receptors using microphysiometry

Meloy

Daniels

Hegde

et al. 2001

British J Pharmacology

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“…The reason for the difference in the Cl and K changes seen after cholinergic and α-adrenergic stimulation cannot be ascertained from the results of this study, but may be related to differences in the extent to which each type of agonist activates intracellular signals regulating ion channels and fluxes. Both Cland K + efflux from salivary cells occur through channels regulated by the level of free cytosolic Ca 2+ (Martinez and Cassity 1986;Martinez and Camden 1989;Martinez 1990) and preliminary evidence indicates that α-adrenergic stimuli cause a smaller increase in cytosolic Ca 2+ than cholinergic stimuli (Hurley and Brinck 1992;Puente et al 1996;Willis et al 1996). This difference in Ca 2+ -mediated signalling could explain the smaller volume of secretion produced by α-adrenergic agonists.…”

Section: Discussionmentioning

confidence: 99%

Effects of cholinergic and α-adrenergic agonists on the monovalent ion content of rat submandibular gland acinar cells studied by X-ray microanalysis

Zhang

Martinez

Roomans

1997

Histochemistry and Cell Biology

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The effects of cholinergic and alpha-adrenergic stimulation (in vivo and in vitro) on the monovalent ion content of rat submandibular gland acinar cells were evaluated at the subcellular level by X-ray microanalysis. Fragments of glands or enzymatically dispersed acini were slam-frozen and cut into ultrathin cryosections. Spectra were collected from secretory granules, nucleus, the basal cytoplasm containing endoplasmic reticulum and the apical cytoplasm identified between secretory granules. No significant changes in Na and Cl content were observed after the isolation of acini, but the K concentration decreased compared with cells from in situ glands. The Cl and K content in all four compartments studied decreased significantly after cholinergic stimulation both in vivo and in vitro but in a more restricted fashion after alpha-adrenergic stimulation. Our findings indicate that: (1) the physiological mechanisms regulating the monovalent ion composition of submandibular cells are relatively well preserved in isolated acinar cells; (2) the results from in vivo experiments are in good agreement with those from in vitro experiments; and (3) the effects of cholinergic and alpha-adrenergic stimulation on the K+ and Cl- efflux at the subcellular level are similar but the response is generally less with alpha-adrenergic stimulation.

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Comparison of calcium mobilization in response to noradrenaline and acetylcholine in submandibular cells of newborn and adult rats

Wells

Zhang

Martinez

1997

Archives of Oral Biology

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Differential regulation of agonist-stimulated Ca2+ influx in acini of rat pancreas and submandibular gland

Cited by 6 publications

References 17 publications

Functional characterization of rat submaxillary gland muscarinic receptors using microphysiometry

Functional characterization of rat submaxillary gland muscarinic receptors using microphysiometry

Effects of cholinergic and α-adrenergic agonists on the monovalent ion content of rat submandibular gland acinar cells studied by X-ray microanalysis

Comparison of calcium mobilization in response to noradrenaline and acetylcholine in submandibular cells of newborn and adult rats

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