Differential Regulation of Adiponectin Secretion from Cultured Human Omental and Subcutaneous Adipocytes: Effects of Insulin and Rosiglitazone

Motoshima, Hiroyuki; Wu, Xiangdong; Sinha, Madhur K.; Hardy, V.Elise; Rosato, Ernest L.; Barbot, Donna J.; Rosato, Francis E.; Goldstein, Barry J.

doi:10.1210/jc.2002-020635

Cited by 393 publications

(319 citation statements)

References 43 publications

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“…21,22 There is also evidence that insulin may have direct effect on adiponectin gene expression and adiponectin concentrations in vitro. [23][24][25] It is therefore conceivable that the higher levels of insulin in insulin-resistant subjects may downregulate levels of adiponectin as suggested previously. 26,27 Although we have shown an inverse association between adiponectin and insulin resistance, it is obvious from Figure 2a that there was overlap of adiponectin levels between insulin-sensitive and insulinresistant patients and some of the insulin-sensitive individuals had low adiponectin.…”

Section: Discussionmentioning

confidence: 82%

Adiponectin, insulin resistance and clinical expression of the metabolic syndrome in patients with Type 2 diabetes

et al. 2006

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Background: Obesity and the metabolic syndrome have emerged as clinical and public health crises in many populations, but not all obese patients have the syndrome. As adipocytes produce several adipokines that modulate insulin action as well as glucose and lipid metabolism, we postulate that estimation of adipokines may be useful addition to the criteria used to identify obese individuals with the metabolic syndrome. Objective: To evaluate the determinants and associations of plasma adiponectin in relation to the metabolic syndrome in patients with Type 2 diabetes. Design: Cross-sectional study. Setting: General Teaching Hospital. Patients: One hundred and thirty five (57 M, 78 F) patients with Type 2 diabetes mellitus. Measurements: Adiponectin, leptin, high-sensitivity C-reactive protein (hs-CRP), fasting plasma insulin, glucose, glycated hemoglobin and full lipid profile. Patients were classified on the basis of the degree of adiposity, insulin resistance (IR) (homeostasis model assessment of insulin resistance (HOMA-IR)) and the number of the American Heart Association and the National Heart, Lung and Blood Institute criteria of the metabolic syndrome. Results: Adiponectin levels were inversely correlated with age, indices of obesity, IR and hs-CRP. Overweight/obese and nonobese insulin-sensitive patients had significantly higher (Po0.05) adiponectin levels than those with IR despite similar body mass index and waist circumference. Therefore, within each category of obesity stratification, lower adiponectin levels were associated with IR. Adiponectin showed stepwise decrease with increasing number of the criteria for diagnosis of the metabolic syndrome. Using multiple logistic regression, the odds ratio of the metabolic syndrome as predicted by adiponectin was 0.73 (95% confidence interval 0.53-0.96; P ¼ 0.04). At cutoff point of 18 ng/ml, the diagnostic sensitivity and specificity of adiponectin for the metabolic syndrome were 83 and 65%, respectively, in male patients and 92 and 41%, respectively, in female patients. Receiver operating characteristic analysis showed that adiponectin had significantly higher area under the curve compared with leptin, leptin:adiponectin ratio and triglycerides for the detection of the metabolic syndrome. Conclusions: In patients with Type 2 diabetes, adiponectin concentrations are closely related to IR and the components of the metabolic syndrome. Adiponectin concentration may be a useful addition to the criteria used for identifying obese subjects with the metabolic syndrome.

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Section: Discussionmentioning

confidence: 82%

Adiponectin, insulin resistance and clinical expression of the metabolic syndrome in patients with Type 2 diabetes

et al. 2006

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“…Adiponectin is an adipokine which decreases when fat accumulates [20]. In this paper, adiponectin was observed as an independent protective factor against MetS and its associated components, owing to the inverse relationship of adiponectin with triglycerides and fasting glucose, and the positive association of adiponectin with HDL cholesterol.…”

Section: Discussionmentioning

confidence: 88%

“…Smokeless tobacco and alcohol consumption have been shown to have a role in obesity and cardiometabolic diseases [18,19]. With increasing fat accumulation, the level of adiponectin (a hormone secreted from adipose tissue) is reduced, suggesting that it may have a role in MetS [20]. Identifying the risk factors influencing body composition and MetS is fundamental to understanding the pathogenesis of these diseases in this group of African women, who seem to be more susceptible to this phenomenon than white women [7].…”

Section: Introductionmentioning

confidence: 99%

Factors associated with obesity and metabolic syndrome in ageing black South African women

Gradidge

2017

Global Health Action

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Background: The incidence of obesity and related metabolic diseases is high and increasing in sub-Saharan African women. Evidence on the determinants of these diseases is limited, particularly in black South African women. Objective: This PhD review attempts to understand the determinants of obesity and metabolic syndrome (MetS) in a population of ageing urban-dwelling black South African women. Methods: Drawing on the longitudinal Birth-to-Twenty-Plus cohort, data were collected in 2002/03 and 2012/13, including information on behavioural factors (smoking, sitting time, physical activity, smokeless tobacco, and alcohol consumption), body-size perception, body composition (measures of adiposity and lean mass), blood pressure, and cardiometabolic biomarkers (lipid profile, fasting insulin, fasting blood glucose, insulin resistance, leptin, and adiponectin). Results: The prevalence of obesity and related cardiovascular disease risk was high and increased significantly over the 10 year period. Despite most of the study population being physically active, sitting time was high and associated with elevated blood pressure and hypertriglyceridaemia. Two groups of people were observed, those who were happy and those who were unhappy with their body size. In logistic regression analysis, the risk of MetS was lowered by abdominal subcutaneous adipose tissue and adiponectin, and increased by age, smoking, truncal lean mass, and insulin resistance. Conclusions: Obesity was confirmed to be increasing in black South African women, despite most women being sufficiently active according to guidelines of ≥150 min activity/week. Nevertheless, the contribution of sitting time to poor health outcomes is evident in this study population and must be addressed, particularly in women who are content with being obese. The novel finding of the effects of abdominal subcutaneous tissue and truncal lean mass with MetS requires further investigation. The protective effect of adiponectin against MetS is an important finding which highlights the novel interaction between adiposity and cardiometabolic diseases in black South African women.

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“…Differences statistically significant compared to non-pregnant with p<0.001 ISI Insulin sensitivity index, HGP hepatic glucose production a Pregnant vs pregravid tion of plasma adiponectin. Total adiponectin, as well as HMW multimers, decrease in response to insulin infusion [6,35,36], but this may be regulated at the level of secretion rather than transcription [37]. Adiponectin is secreted as HMW and LMW multimeric complexes, and the amount of circulating complexes is likely to be regulated at the tissue level because LMW and HMW complexes do not interchange in the serum [38].…”

Section: Regulation Of Adiponectin Expressionmentioning

confidence: 99%

Adiponectin in human pregnancy: implications for regulation of glucose and lipid metabolism

et al. 2006

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Aims/hypothesis: Adiponectin is upregulated during adipogenesis and downregulated in insulin-resistant states. The mechanism(s) governing the re-arrangements from adipogenesis to facilitated lipolysis during pregnancy are unknown. Our purpose was to analyse the role of adiponectin relative to the metabolic changes in human pregnancy. Subjects, materials and methods: Lean women (BMI <25 kg/m²) were evaluated longitudinally before conception, and in early (12-14 weeks) and late (34-36 weeks) pregnancy. Insulin sensitivity was measured using the glucose clamp technique. Venous blood and subcutaneous adipose tissue biopsies were obtained at each time point. Results: Adiponectin concentrations were lower in the third trimester than in the pregravid condition (9.9±1.4 vs 13.5±1.8 μg/ml). The hypoadiponectinaemia was reflected by a 2.5-fold decrease in white adipose tissue adiponectin mRNA. These changes were associated with a 25% increase in fat mass (23.7±2.9 vs 18.9±2.9 kg). Insulin infusion decreased high molecular weight adiponectin complexes in pregravid women (9.9±0.6 vs 6.2±0.06) and the suppressive effect of insulin was lost during pregnancy. The pregnancy-mediated changes in adiponectin were strongly correlated with basal insulin levels and insulin sensitivity (p<0.0001). The relationship between adiponectin and insulin sensitivity was related to the decreased insulin regulation of glucose utilisation (r=0.55, p<0.001) but not of endogenous hepatic glucose production. Conclusions/interpretation: These data demonstrate that pregnancy is associated with adiponectin changes in lean women. Hypoadiponectinaemia is reflected by a lower amount of high molecular weight adiponectin and by the ratio of high to low molecular weight multimers. The adiponectin changes relate to decreased insulin sensitivity of glucose disposal rather than alterations of lipid metabolism.

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Differential Regulation of Adiponectin Secretion from Cultured Human Omental and Subcutaneous Adipocytes: Effects of Insulin and Rosiglitazone

Cited by 393 publications

References 43 publications

Adiponectin, insulin resistance and clinical expression of the metabolic syndrome in patients with Type 2 diabetes

Adiponectin, insulin resistance and clinical expression of the metabolic syndrome in patients with Type 2 diabetes

Factors associated with obesity and metabolic syndrome in ageing black South African women

Adiponectin in human pregnancy: implications for regulation of glucose and lipid metabolism

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