1995
DOI: 10.1074/jbc.270.43.25672
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Differential Recognition of β-Lactam Antibiotics by Intestinal and Renal Peptide Transporters, PEPT 1 and PEPT 2

Abstract: This study was initiated to determine if there are differences in the recognition of ␤-lactam antibiotics as substrates between intestinal and renal peptide transporters, PEPT 1 and PEPT 2. Reverse transcription-coupled polymerase chain reaction and/or Northern blot analysis have established that the human intestinal cell line Caco-2 expresses PEPT 1 but not PEPT 2, whereas the rat proximal tubule cell line SKPT expresses PEPT 2 but not PEPT 1. Detailed kinetic analysis has provided unequivocal evidence for pa… Show more

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Cited by 250 publications
(171 citation statements)
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References 24 publications
(23 reference statements)
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“…Under these conditions, cells reached confluence the next day. Uptake of [ 14 C]Gly-Sar was measured at room temperature on the seventh to eighth day after seeding as described earlier [12,13]. The uptake buffer contained 25 mM Mes/Tris, pH 6.0, 140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl 2 , 0.8 mM MgSO 4 , 5 mM glucose.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Under these conditions, cells reached confluence the next day. Uptake of [ 14 C]Gly-Sar was measured at room temperature on the seventh to eighth day after seeding as described earlier [12,13]. The uptake buffer contained 25 mM Mes/Tris, pH 6.0, 140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl 2 , 0.8 mM MgSO 4 , 5 mM glucose.…”
Section: Methodsmentioning
confidence: 99%
“…Caco-2 cells (passages 35Ϫ53) were routinely cultured as described previously [12,13] in 75-cm 2 culture flasks with minimum essential medium supplemented with 10% fetal bovine serum, penicillin (100 units/ml), streptomycin (100 µg/ml), and 1% non-essential amino acid solution. Subconfluent cultures were treated for 5 min with Dulbecco's phosphate-buffered saline without Ca ϩϩ and Mg ϩϩ , pH 7.2 followed by a 1-min incubation with 0.05% trypsin/EDTA solution.…”
Section: Methodsmentioning
confidence: 99%
“…Notably, the SLC15A2 haplotype block in a Japanese cohort was more similar to African-American and European-American populations (Figures 1 and 2a) 8 were in absolute linkage disequilibrium (r 2 ¼1) (Supplementary Figure 1a). SLC15A2 has been shown to recognize a wide spectrum of pharmacologically active compounds, including b-lactam antibiotics, 22 inhibitors of the angiotensin converting enzyme 23,24 and non-peptidic drugs. 24 Genetic variations in SLC15A2 (F350L, S409P and K509R) displayed different K m values for glycyl-sarcosine.…”
Section: Resultsmentioning
confidence: 99%
“…However, only a few cephalosporins (cephalexin and cefadroxil) have been reported to be substrates of PEPT2. 34 Therapeutic use of cephaloridine in humans has been found to cause nephrotoxicity.…”
Section: ) Elimination From the Kidneymentioning
confidence: 99%