Differential recognition of the 52-kd Ro(SS-A) antigen by sera from patients with primary biliary cirrhosis and primary Sjogren's syndrome

Anti‐SLA/LP alone or in combination with anti‐Ro52 and fine specificity of anti‐Ro52 antibodies in patients with autoimmune hepatitis

“…The CC domain is the most commonly targeted region of the Ro52 irrespective of the underlying disease, while SS patients seem to have specificities against several different Ro52 epitopes including the RING, B-box and CC domains (25,43,44). In PBC, a small Ro52 epitope at CC domain (aa228-245) has been identified as dominant using different fusion proteins in ELISA and immunoblotting (45).…”

B-cell epitopes of the intracellular autoantigens Ro/SSA and La/SSB: Tools to study the regulation of the autoimmune response

“…The major immunoreactivity of Ro 52kD autoantigen was localized, using recombinant Ro52 fusion proteins, in the middle coiled-coil region of Ro52 [1]. The 190e245aa region of the amino acid sequence was reactive with almost all anti-Ro52 positive sera and was independent of associated diseases [24]. An epitope spanning the 200e239aa of Ro52, which contains the complete leucine zipper motif, has been also identified in the same region [25].…”

“…Anti-Ro52 antibodies are also found in primary biliary cirrhosis associated with sicca syndrome. The anti-Ro52 antibodies in this setting are directed against a smaller epitope than in primary Sjögren's syndrome [24].…”