Differential reactivity of serum immunoglobulins from Brazilian wild mammals to staphylococcal A and streptococcal G proteins

Pelli, Afonso; Castellano, Lúcio Roberto Cançado; Cardoso, Marcos R. S.; Vasconcelos, Luís A. S.; Domingues, Marcos Aurélio; Ferreira, Maria B.; Rodrigues, Virmondés

doi:10.1177/1040638711434322

Cited by 6 publications

(2 citation statements)

References 12 publications

(13 reference statements)

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“…Evolutionary distance is likely to play a significant role for inter-species variation in binding patterns with protein A/G in marsupials due to variation of protein A/G binding domains present in immunoglobulins [ 31 , 39 ]. Similar differences along taxonomic lines are observed between eutherian mammal species [ 31 , 40 ]. However, the results presented here show that taxonomic distance is not always a reliable predictor of immunoglobulin-binding affinity.…”

Section: Discussionsupporting

confidence: 72%

“…However, the results presented here show that taxonomic distance is not always a reliable predictor of immunoglobulin-binding affinity. Therefore, it is important to assess the species-specific binding capability of protein A/G before developing serological assays [ 40 ] or use any commercially available kits using protein A/G as a conjugate, particularly with wildlife species where limited affinity data is available.…”

Section: Discussionmentioning

confidence: 99%

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Towards a pan marsupial sero-immunological tool in the demanding field of wildlife serology: Marsupial immunoglobulin-binding capability with protein A/G, protein L and anti-kangaroo antibody

Liyanage,

Vaz,

Jabbar

et al. 2023

PLoS ONE

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Detection of infections in wildlife species is increasingly important to reduce the risk of spreading zoonotic and economically important parasites, understand disease epidemiology and promote the conservation of wildlife species. Serological tests are key in disease diagnosis and surveillance by detecting immunoglobulins against infectious agents. However, the need for species-specific reagents has limited the application of serological tests in wildlife species. This study evaluated the serum immunoglobulin-binding capability of polyclonal anti-kangaroo antibody and two non-species-specific reagents, including protein A/G and protein L, with the largest range of Australian marsupial species so far, including 32 species representing three major marsupial orders. Immunoglobulin-binding capability was assessed using immunoblotting, enzyme-linked immunosorbent assay and Western blot techniques. Variation in immunoglobulin-binding capability was observed between the three reagents and across the species tested, both across but also within taxonomic groups. Taxonomic distance was thus not always a good predictor of immunoglobulin-binding affinity, emphasizing the need to validate these reagents for each species separately. However, all three reagents bound with the serum immunoglobulins of most marsupial species tested. The findings of this study provide a valuable reference for species differences in affinity to protein A/G, protein L and anti-kangaroo antibody, assisting in the selection of appropriate reagents and the development of sero-immunological assays in Australian marsupials.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Towards a pan marsupial sero-immunological tool in the demanding field of wildlife serology: Marsupial immunoglobulin-binding capability with protein A/G, protein L and anti-kangaroo antibody

Liyanage,

Vaz,

Jabbar

et al. 2023

PLoS ONE

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Wild Animals as Sentinels of Paracoccidioides brasiliensis in the State of Rio Grande do Sul, Brazil

et al. 2014

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Paracoccidioides brasiliensis, a dimorphic pathogenic fungus, causes the principal form of systemic mycosis in Brazil. The literature furnishes only limited data on the ecology of this fungus in the state of Rio Grande do Sul, the southernmost state of Brazil. The purpose of this study was to evaluate the prevalence of fungal infection in wild animals, using serological tests and using the animals as sentinels of the presence of P. brasiliensis in three specified mesoregions of Rio Grande do Sul. A total of 128 wild animals from the three mesoregions were included in the study. The serum samples were evaluated by immunodiffusion and the enzyme-linked immunosorbent assay (ELISA) technique to detect anti-gp43 antibodies from P. brasiliensis. Two conjugates were tested and compared with the ELISA technique. Although no positive samples were detected by immunodiffusion, 26 animals (20%), belonging to 13 distinct species, were found to be seropositive by the ELISA technique. The seropositive animals were from two mesoregions of the state. The results were similar according to the gender, age, and family of the animals, but differed significantly according to the conjugate used (p < 0.001), showing more sensitivity to protein A-peroxidase than to protein G-peroxidase. The finding that wild animals from the state of Rio Grande do Sul are exposed to P. brasiliensis suggests that the fungus can be found in this region despite the often-rigorous winters, which frequently include below-freezing temperatures.

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Improving the diagnosis of bovine tuberculosis using gold nanoparticles conjugated with purified protein derivative: special regard to staphylococcal protein A and streptococcal protein G

El-Sayyad

Hasan²,

Saad³

et al. 2021

Environ Sci Pollut Res

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Differential reactivity of serum immunoglobulins from Brazilian wild mammals to staphylococcal A and streptococcal G proteins

Cited by 6 publications

References 12 publications

Towards a pan marsupial sero-immunological tool in the demanding field of wildlife serology: Marsupial immunoglobulin-binding capability with protein A/G, protein L and anti-kangaroo antibody

Towards a pan marsupial sero-immunological tool in the demanding field of wildlife serology: Marsupial immunoglobulin-binding capability with protein A/G, protein L and anti-kangaroo antibody

Wild Animals as Sentinels of Paracoccidioides brasiliensis in the State of Rio Grande do Sul, Brazil

Improving the diagnosis of bovine tuberculosis using gold nanoparticles conjugated with purified protein derivative: special regard to staphylococcal protein A and streptococcal protein G

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