Differential Reactivity in Epidermal Cells of <i>Begonia rex</i> Excised and Grown <i>in vitro</i>

Chlyah, Averil; Van, Marie Tran Thanh

doi:10.1111/j.1399-3054.1975.tb03860.x

Cited by 37 publications

(9 citation statements)

References 6 publications

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“…Explants excised from floral branches, composed of 3 -6 layers of epidermal and subepidermal cells, were capable of either flower or shoot bud formation. The system was also applied in shoot regeneration of Begonia rex [4], Torenia fournieri [3], Bryophyllum daigremontianum [1] and Psophocarpus tetragonolobus [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

High frequency plant regeneration from thin cell layer explants of Brassica napus

Klimaszewska¹,

Keller²

1985

Plant Cell Tiss Organ Cult

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Explants composed of the epidermis and 4-9 layers of subepidermal cells were excised from intelnodes of Brassica napus L. ssp. oleifera cv. Westar and cultured on modified Murashige and Skoog (MS) medium. The three or four terminal internodes excised from plants at an early stage (before any flower buds had opened) were shown to be the best explant source. Both cytokinin and auxin were required for induction of shoot organogenesis. Of six auxins tested, only naphthaleneacetic acid (NAA) was effective in shoot bud initiation. All four cytokinins tested (when associated with 0.5 mg1-1 NAA) promoted organogenesis, but at differing frequencies. The highest shoot induction frequency was obtained at 10-15 mgl -t benzyladenine (BA). The organogenic response was strongly affected by the nitrogen content of the medium. The best response was observed when NO; was the sole nitrogen source (supplied as KNO 3) in the range 30-90mM. Sucrose and glucose were equally supportive in shoot regeneration with the optimal levels at 0.12 M and 0.15 M, respectively. Shoots were rooted on medium free of growth regulators and mature plants were grown in the greenhouse. Plants were also recovered from leafy structures which differed morphologically and histologically from shoot buds.

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Section: Introductionmentioning

confidence: 99%

High frequency plant regeneration from thin cell layer explants of Brassica napus

Klimaszewska¹,

Keller²

1985

Plant Cell Tiss Organ Cult

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“…(Tran Thanh Van 1980a). In monolayers of Begonia rex epidermal cells (Tran Thanh Van et al 1974, Chlyah andTran Thanh Van 1975) and in thin cell layers of tobacco (Tran Thanh Trinh 1978a, Tran Thanh Van 1980a, b) cultured under the same respective chemical conditions, it has been shown that unicellular hair differentiation and flower neoformation are affected by temperature and agar concentration, respectively.…”

Section: Introductionmentioning

confidence: 99%

In vitro control of de novo flower differentiation from tobacco thin cell layers cultured on a liquid medium

Cousson¹,

Van²

1981

Physiologia Plantarum

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Thin cell layers of tobacco (three to six layers of epidermal and subepidermal cells) were allowed to float on the surface of a liquid medium. Whereas the control on an identical medium solidified by agar gave 100% of explant‐forming flowers, no flowers formed in the absence of agar (100% of the explants formed buds). In order to initiate flower formation, various modifications of the liquid medium were tried: different ratios of indolyl‐3‐butyric acid (IBA) to kinetin (Kin) from 1 to 100, a range of pH from 4.0 to 7.0, and glass beads of different diameters (in an attempt to change the physico‐chemical characteristics of the culture substrate itself). On a liquid medium with glass beads, four types of morphogenesis (flowers, buds, a mixed programme of flowers and buds, and a non‐morphogenetic programme –i.e. the explants remained unchanged) were separately induced by changing one of the three factors: pH, IBA and Kin in equimolar concentrations, and diameter of the glass beads. Changing only the IBA/Kin ratio failed to provoke flower differentiation. pH of the medium was found to change after autoclaving and the effect of glass beads on morphogenesis was partly related to modification of pH.

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“…and 10 g/l agar, to which 1 µM BA was added for bud formation, or 0.5 µM NAA for root formation, or 0.1 µM NAA for unicellular hair formation. This new morphogenetic program, expressed after 6 days of culture, represents one of the simplest types of differentiation (Chlyah and Tran Thanh Van, 1975).…”

Section: Begonia (Begonia Rex) Over 2000 Species Of Begoniamentioning

confidence: 99%

Thin Cell Layer technology in ornamental plant micropropagation and biotechnology

Silva¹

2003

Afr. J. Biotechnol.

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Thin cell layer (TCL) technology originated almost 30 years ago with the controlled development of flowers, roots, shoots and somatic embryos on tobacco pedicel longitudinal TCLs. Since then TCLs have been successfully used in the micropropagation of many ornamental plant species whose previous in vitro regeneration was not successful using conventional methods. This review examines the fundamentals behind TCLs, and their application in ornamental plant micropropagation and transformation.

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Differential Reactivity in Epidermal Cells of Begonia rex Excised and Grown in vitro

Cited by 37 publications

References 6 publications

High frequency plant regeneration from thin cell layer explants of Brassica napus

High frequency plant regeneration from thin cell layer explants of Brassica napus

In vitro control of de novo flower differentiation from tobacco thin cell layers cultured on a liquid medium

Thin Cell Layer technology in ornamental plant micropropagation and biotechnology

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