Differential quantitative proteomics study of experimental Mannheimia haemolytica mastitis in sheep

Katsafadou, Angeliki I.; Tsangaris, George Th.; Anagnostopoulos, Athanasios K.; Billinis, Charalambos; Barbagianni, M.S.; Vasileiou, N.G.C.; Spanos, Stavros A.; Mavrogianni, V.S.; Fthenakis, George C.

doi:10.1016/j.jprot.2019.103393

Cited by 19 publications

(35 citation statements)

References 122 publications

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“…In both experiments, mastitis was induced, which allowed us to have animals with confirmed disease under fully controlled conditions. From the results of previous experiments [7,17], cathelicidin-1 was singled out for a detailed investigation, particularly during the initial stage of mammary infection. In experiment 2, samples were collected from experimental animals at frequent intervals for 24 h after inoculation.…”

Section: Discussionmentioning

confidence: 99%

“…In experiment 1, ewes were challenged as follows [17,20]. Initially, the teat was disinfected by using iodine povidone solution.…”

Section: Inoculation Proceduresmentioning

confidence: 99%

“…In experiment 1, udder examination of ewes was performed immediately prior to challenge (D0) as well as 12 h after challenge (D0 + 12 h) and 1, 2, 3, and 4 days after challenge (D1, D2, D3, D4) (after the D3 sampling, three ewes were withdrawn from the experiment in order to perform a mammary biopsy to obtain a tissue sample that was used for histological confirmation of mastitis, whilst the other two ewes were subjected to mammary biopsy after the D4 sampling [17]). In experiment 2, udder examination was performed immediately prior to challenge (D0) as well as 3, 6, 9, 12 h and 1 d after challenge (D0 + 3 h, D0 + 6 h, D0 + 9 h, D0 + 12 h, D1).…”

Section: Animal Examination Sampling Conventional Laboratory Examinmentioning

confidence: 99%

“…Mammary tissue samples collected by biopsy were fixed in 10% neutral-buffered formalin and processed by standard histological techniques [17].…”

Section: Animal Examination Sampling Conventional Laboratory Examinmentioning

confidence: 99%

“…From each original sample, many Eppendorf tubes were filled with samples of whey for performing duplicate proteomics examinations. All samples were appropriately prepared for proteomics evaluation as described in detail by Katsafadou et al [17]. Protein content was determined by the Bradford assay [33].…”

Section: Proteomics Analysismentioning

confidence: 99%

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Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

et al. 2019

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The objective of the study was the investigation of the behaviour of cathelicidin-1 in the milk after experimental infection with two prominent bacterial pathogens (experiment 1: Mannheimia haemolytica, experiment 2: M. haemolytica and Staphylococcus chromogenes) as a potential early indicator for diagnosis of mastitis in sheep. In two experiments, after bacterial inoculation into the udder of ewes, bacteriological and cytological examinations of milk samples as well as proteomics examinations [two-dimensional gel electrophoresis analysis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis] were performed sequentially. Cathelicidin-1 was detected and spot densities obtained from PDQuest v.8.0 were recorded. Associations were calculated between cell content and spot densities as well as between presence of mastitis in a mammary gland at a given time-point and detection of cathelicidin-1 in the respective milk sample. All inoculated mammary glands developed mastitis, confirmed by the consistent bacterial isolation from mammary secretion and increased leucocyte content therein. Spot density of cathelicidin-1 in samples from inoculated glands increased 3 h post-inoculation; spot density of cathelicidin-1 in samples from inoculated glands was higher than in samples from uninoculated controls. There was clear evidence of correlation between cell content and cathelicidin-1 spot densities in milk samples. There was significant association between presence of mastitis in the mammary gland and detection of cathelicidin-1 in the respective milk sample; overall accuracy was 0.818—this was significantly greater during the first 24 h post-challenge (0.903) than after the first day (0.704). In conclusion, detection of cathelicidin-1 in milk was significantly associated with presence of mastitis in ewes. The associations were stronger during the first 24 h post-infection than after the first day. Cathelicidin-1 has the advantage that it can be a non-specific biomarker, as simply a “positive”/“negative” assessment would be sufficient.

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Section: Discussionmentioning

confidence: 99%

“…In experiment 1, ewes were challenged as follows [17,20]. Initially, the teat was disinfected by using iodine povidone solution.…”

Section: Inoculation Proceduresmentioning

confidence: 99%

Section: Animal Examination Sampling Conventional Laboratory Examinmentioning

confidence: 99%

“…Mammary tissue samples collected by biopsy were fixed in 10% neutral-buffered formalin and processed by standard histological techniques [17].…”

Section: Animal Examination Sampling Conventional Laboratory Examinmentioning

confidence: 99%

Section: Proteomics Analysismentioning

confidence: 99%

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Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

et al. 2019

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Proteomics and Peptidomics for Food Safety

Mora

Toldrá

2021

Comprehensive Foodomics

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Proteomics data of ovine mastitis associated with Mannheimia haemolytica

et al. 2019

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Proteomics data have been obtained from experimental mastitis in ewes after intramammary challenge with Mannheimia haemolytica . Animals were sampled before and sequentially after challenge; blood plasma and milk whey samples were produced and were subjected to proteomics evaluation by means of two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Full protein maps and differential proteomics in sequential samples from blood plasma and milk whey of experimental ewes were presented. Post-challenge, 33 and 89 proteins were identified with differential abundance in blood plasma and milk whey, respectively. Also, 74 proteins were identified with differential abundance between the inoculated and contralateral glands. The data provide further insight in the pathogenesis of mastitis in sheep and indicate potential biomarkers for the disease. The data are further discussed in the research article “Differential quantitative proteomics study of experimental Mannheimia haemolytica mastitis in sheep” [1].

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Differential quantitative proteomics study of experimental Mannheimia haemolytica mastitis in sheep

Cited by 19 publications

References 122 publications

Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

Proteomics and Peptidomics for Food Safety

Proteomics data of ovine mastitis associated with Mannheimia haemolytica

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