Differential pulse polarography for monitoring cell cultures

Abstract

The differential pulse polarography has been used on samples taken from the medium during the culture of chinese hamster ovary cells with a view to producing the active principle of an anti-cancer drug. An electrochemical signal at a potential of -1.8 V with respect to the saturated calomel electrode was correlated with the lactic acid concentration, the pH variation and the number of cells. The peaks observed in oxidation at potentials -0.35 V and -0.05 V were associated with the transformation of the cystine present in the nutrient medium into cysteine and provide an indication of the modification of the reducing power of the medium during culture. No correlation was found between the electrochemical behaviour and cell production.

Antiproliferative effects of SR31747A in animal cell lines are mediated by inhibition of cholesterol biosynthesis at the sterol isomerase step

“…MCF7 cells were grown in RPMI 1640 supplemented with bovine insulin (10 µg/ml) and human transferrin (10 µg/ml). CHO and M1 cell lines were grown in defined medium number one for CHO (MDC1) [11] supplemented with bovine insulin (1 µg/ml) and human transferrin (3 µg/ml). For each cell line, we verified that the cells were able to proliferate in the defined medium (data not shown).…”