Differential proteomics reveals the hallmarks of seed development in common bean ( Phaseolus vulgaris L.)

Parreira, José Ricardo; Bouraada, J; Fitzpatrick, Martin; Silvestre, Susana; Silva, Anabela Bernardes da; Silva, Jorge Marques da; Almeida, André M.; Fevereiro, Pedro; Altelaar, Maarten; Araújo, Susana de Sousa

doi:10.1016/j.jprot.2016.03.002

Cited by 23 publications

(41 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, among transcripts related to protein degradation, an unusually high ratio of terms related to proteasomal degradation to those standing for cytoplasmic proteases occurred at 20 DAP. Both protein categories have been reported to be promoted in both maturation and desiccation stages in Cruciferae [79,80] and at the desiccation onset in common bean (Phaseolus vulgaris) [32], though the most active turnover has been reported for early pre-maturation stages [29,32]. In contrast to apparently high rates of proteasomal activity, ribosomal biosynthesis decreased in Sprint-2, which serves as a marker of the end of maturation.…”

Section: Discussionmentioning

confidence: 99%

“…To date, studies dedicated to the transcriptomic or proteomic profiling of developing seeds have been performed for several legume species, including the chickpea [26], soybean [27,28], barrel medic [29][30][31], common bean [13,32], and garden pea [33][34][35]. These large-scale assays allow global genetic programs involved in seed maturation to be dissected, and expression changes to be traced over time.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomic Insights into Mechanisms of Early Seed Maturation in the Garden Pea (Pisum sativum L.)

Malovichko

Shtark

Vasileva

et al. 2020

Cells

View full text Add to dashboard Cite

The garden pea (Pisum sativum L.) is a legume crop of immense economic value. Extensive breeding has led to the emergence of numerous pea varieties, of which some are distinguished by accelerated development in various stages of ontogenesis. One such trait is rapid seed maturation, which, despite novel insights into the genetic control of seed development in legumes, remains poorly studied. This article presents an attempt to dissect mechanisms of early maturation in the pea line Sprint-2 by means of whole transcriptome RNA sequencing in two developmental stages. By using a de novo assembly approach, we have obtained a reference transcriptome of 25,756 non-redundant entries expressed in pea seeds at either 10 or 20 days after pollination. Differential expression in Sprint-2 seeds has affected 13,056 transcripts. A comparison of the two pea lines with a common maturation rate demonstrates that while at 10 days after pollination, Sprint-2 seeds show development retardation linked to intensive photosynthesis, morphogenesis, and cell division, and those at 20 days show a rapid onset of desiccation marked by the cessation of translation and cell anabolism and accumulation of dehydration-protective and -storage moieties. Further inspection of certain transcript functional categories, including the chromatin constituent, transcription regulation, protein turnover, and hormonal regulation, has revealed transcriptomic trends unique to specific stages and cultivars. Among other remarkable features, Sprint-2 demonstrated an enhanced expression of transposable element-associated open reading frames and an altered expression of major maturation regulators and DNA methyltransferase genes. To the best of our knowledge, this is the first comparative transcriptomic study in which the issue of the seed maturation rate is addressed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic Insights into Mechanisms of Early Seed Maturation in the Garden Pea (Pisum sativum L.)

Malovichko

Shtark

Vasileva

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…Plant seed maturation and transition to dormancy are accompanied by the desiccation that poses a stressful condition to all the cell components. Genome stability during desiccation is maintained via expression of genes related to the DNA repair and chromatin remodeling 38,39 as well as chromatin compaction 40 Interestingly, structurally similar β-barrel domain-containing proteins that belong to Omp (outer membrane protein) superfamily of Proteobacteria also form amyloids 42,43 . Thus, several eukaryotic and prokaryotic proteins containing β-barrel domains are amyloidforming and these domains are likely to be important amyloidogenic determinants.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of storage proteins in plant seeds is mediated by amyloid formation

Antonets

Белоусов

Sulatskaya

et al. 2019

Preprint

View full text Add to dashboard Cite

Amyloids are protein aggregates with a highly ordered spatial structure giving them unique physicochemical properties. Different amyloids not only participate in the development of numerous incurable diseases but control vital functions in Archaea, Bacteria and Eukarya. Plants are a poorly studied systematic group in the field of amyloid biology. Amyloid properties have not yet been demonstrated for plant proteins under native conditions in vivo. Here we show that seeds of garden pea Pisum sativum L. contain amyloid-like aggregates of storage proteins, the most abundant one, 7S globulin Vicilin, forms bona fide amyloids in vivo and in vitro. The Vicilin amyloid accumulation increases during seed maturation and wanes at germination. Amyloids of Vicilin resist digestion by gastrointestinal enzymes, persist in canned peas and exhibit toxicity for yeast and mammalian cells. Our finding for the first time reveals involvement of amyloid formation in the accumulation of storage proteins in plant seeds.

show abstract

“…[ 13 ] Seed developmental events were characterized at protein levels in Medicago truncatula , Phaseolus vulgaris, Arachis hypogaea , Pisum sativum, Nelumbo nucifera , Glycine max, Vigna radiata , etc. [ 14–17 ] Protein phosphorylation is commonly involved in key regulatory process, which mediates complex signaling networks in storage organ, seed. [ 18 ] Till date only phosphoproteome of N. nucifera and G. max seeds were investigated in Fabaceae family.…”

Section: Introductionmentioning

confidence: 99%

Integrated Seed Proteome and Phosphoproteome Analyses Reveal Interplay of Nutrient Dynamics, Carbon–Nitrogen Partitioning, and Oxidative Signaling in Chickpea

et al. 2020

View full text Add to dashboard Cite

Nutrient dynamics in storage organs is a complex developmental process that requires coordinated interactions of environmental, biochemical, and genetic factors. Although sink organ developmental events have been identified, understanding of translational and post-translational regulation of reserve synthesis, accumulation, and utilization in legumes is limited. To understand nutrient dynamics during embryonic and cotyledonary photoheterotrophic transition to mature and germinating autotrophic seeds, an integrated proteomics and phosphoproteomics study in six sequential seed developmental stages in chickpea is performed. MS/MS analyses identify 109 unique nutrient-associated proteins (NAPs) involved in metabolism, storage and biogenesis, and protein turnover. Differences and similarities in 60 nutrient-associated phosphoproteins (NAPPs) containing 93 phosphosites are compared with NAPs. Data reveal accumulation of carbon-nitrogen metabolic and photosynthetic proteoforms during seed filling. Furthermore, enrichment of storage proteoforms and protease inhibitors is associated with cell expansion and seed maturation. Finally, combined proteoforms network analysis identifies three significant modules, centered around malate dehydrogenase, HSP70, triose phosphate isomerase, and vicilin. Novel clues suggest that ubiquitin-proteasome pathway regulates nutrient reallocation. Second, increased abundance of NAPs/NAPPs related to oxidative and serine/threonine signaling indicates direct interface between redox sensing and signaling during seed development. Taken together, nutrient signals act as metabolic and differentiation determinant governing storage organ reprogramming.

show abstract

Differential proteomics reveals the hallmarks of seed development in common bean ( Phaseolus vulgaris L.)

Cited by 23 publications

References 82 publications

Transcriptomic Insights into Mechanisms of Early Seed Maturation in the Garden Pea (Pisum sativum L.)

Transcriptomic Insights into Mechanisms of Early Seed Maturation in the Garden Pea (Pisum sativum L.)

Accumulation of storage proteins in plant seeds is mediated by amyloid formation

Integrated Seed Proteome and Phosphoproteome Analyses Reveal Interplay of Nutrient Dynamics, Carbon–Nitrogen Partitioning, and Oxidative Signaling in Chickpea

Contact Info

Product

Resources

About