Differential proteomics: Effect of growth regulators on salt stress responses in safflower seedlings

Shaki, Fatemeh; Maboud, Hasan Ebrahimzadeh; Niknam, Vahid

doi:10.1016/j.pestbp.2020.01.006

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…Whether the decreased photosynthesis rate was related to both stomatal function (opening and closing) depended on the duration of salt stress, salt concentration, and crop species ( Figure 1 ). The effect of stomatal and nonstomatal limitation mostly contributes to the changes of net photosynthetic rates in plants ( Shaki et al., 2020 ; Navarro-León et al., 2021 ). However, whether the stomatal and nonstomatal limitation could decrease the photosynthetic rate of seedlings required further investigation.…”

Section: Discussionmentioning

confidence: 99%

The transcriptional regulatory network of hormones and genes under salt stress in tomato plants (Solanum lycopersicum L.)

Wang

Wang²,

Yang³

et al. 2023

Front. Plant Sci.

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Salt stress has become one of the main limiting factors affecting the normal growth and development of tomatoes as well as fruit quality and yields. To further reveal the regulatory relationships between tomato hormones under salt stress, the interaction between hormones and TF and the genome-wide gene interaction network were analyzed and constructed. After salt treatment, the levels of ABA, SA, and JA were significantly increased, the levels of GA were decreased, and IAA and tZ showed a trend of first increasing and then decreasing. The expression patterns of hormone biosynthesis and signal transduction related genes were analyzed based on RNA-seq analysis, the co-expression network of hormones and genome-wide co-expression networks were constructed using weighted gene co-expression network analysis (WGCNA). The expression patterns of specific transcription factors under salt stress were also systematically analyzed and identified 20 hormone-related candidate genes associated with salt stress. In conclusion, we first revealed the relationship between hormones and genes in tomatoes under salt stress based on hormone and transcriptome expression profiles and constructed a gene regulatory network. A transcriptional regulation model of tomato consisted of six types of hormones was also proposed. Our study provided valuable insights into the molecular mechanisms regulating salt tolerance in tomatoes.

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Section: Discussionmentioning

confidence: 99%

The transcriptional regulatory network of hormones and genes under salt stress in tomato plants (Solanum lycopersicum L.)

Wang

Wang²,

Yang³

et al. 2023

Front. Plant Sci.

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“…Some fungi exhibit a symbiotic relationship with certain plants, during which they modulate carbohydrate metabolism, particularly glucose, sucrose, and starch, by interfering with key metabolic pathways such as glycolysis, thereby impacting the host plant’s energy dynamics [ 16 , 17 ]. SA has been demonstrated to promote the synthesis of glucose and sucrose, while simultaneously inhibiting the tricarboxylic acid cycle (TCA) [ 52 , 53 , 54 ]. It regulates sucrose metabolism by modulating key enzymes, including invertase and sucrose synthase, thereby providing crucial energy and carbon sources for defence responses [ 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

Paraphoma chrysanthemicola Affects the Carbohydrate and Lobetyolin Metabolism Regulated by Salicylic Acid in the Soilless Cultivation of Codonopsis pilosula

Sun,

Luo,

et al. 2024

Biology

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Paraphoma chrysanthemicola, an endophytic fungus isolated from the roots of Codonopsis pilosula, influences salicylic acid (SA) levels. The interaction mechanism between SA and P. chrysanthemicola within C. pilosula remains elusive. To elucidate this, an experiment was conducted with four treatments: sterile water (CK), P. chrysanthemicola (FG), SA, and a combination of P. chrysanthemicola with salicylic acid (FG+SA). Results indicated that P. chrysanthemicola enhanced plant growth and counteracted the growth inhibition caused by exogenous SA. Physiological analysis showed that P. chrysanthemicola reduced carbohydrate content and enzymatic activity in C. pilosula without affecting total chlorophyll concentration and attenuated the increase in these parameters induced by exogenous SA. Secondary metabolite profiling showed a decrease in soluble proteins and lobetyolin levels in the FG group, whereas SA treatment led to an increase. Both P. chrysanthemicola and SA treatments decreased antioxidase-like activity. Notably, the FG group exhibited higher nitric oxide (NO) levels, and the SA group exhibited higher hydrogen peroxide (H2O2) levels in the stems. This study elucidated the intricate context of the symbiotic dynamics between the plant species P. chrysanthemicola and C. pilosula, where an antagonistic interaction involving salicylic acid was prominently observed. This antagonism was observed in the equilibrium between carbohydrate metabolism and secondary metabolism. This equilibrium had the potential to engage reactive oxygen species (ROS) and nitric oxide (NO).

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“…In addition, carbohydrate metabolism regulates the synthesis and conversion of sugars, and environmental stresses can disrupt carbohydrate metabolism in plant species [67]. It has been reported that glycoside hydrolase is among the key enzymes in carbohydrate metabolism, which can affect cellulose biosynthesis, such as β-D-xylosidase and xyloglucan transglycosidase/hydrolase, which is involved in sugar biosynthesis and remodeling, and glycoside hydorlase has been identified in the seed development process of many species [68].…”

Section: Salt Stress Induces Changes In Energy Production and Carbohy...mentioning

confidence: 99%

Physiological and Proteomic Analysis of Seed Germination under Salt Stress in Mulberry

Wang¹,

Jiang²,

Cheng³

et al. 2023

Front. Biosci. (Landmark Ed)

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Background: Salinity is the main abiotic stress that affects seed germination, plant growth and crop production. Plant growth begins with seed germination, which is closely linked to crop development and final yields. Morus alba L. is a well-known saline-alkaline tree with economic value in China, and the most prominent method of expanding mulberry tree populations is seed propagation. Understanding the molecular mechanism of Morus alba L. salt tolerance is crucial for identifying salt-tolerant proteins in seed germination. Here, we explored the response mechanism of mulberry seed germination to salt stress at physiological and protein omics levels. Methods: Tandem mass tag (TMT)-based proteomic profiling of Morus alba L. seeds germinated under 50 mM and 100 mM NaCl treatment for 14 days was performed, and the proteomic findings were validated through parallel reaction monitoring (PRM). Results: Physiological data showed that salt stress inhibited the germination rate and radicle length of mulberry seeds, decreased the malondialdehyde (MDA) content and significantly increased superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities. Then, a TMT marker technique was used to analyze the protein groups in mulberry seeds with two salt treatment stages, and 76,544 unique peptides were detected. After removing duplicate proteins, 7717 proteins were identified according to TMT data, and 143 (50 mM NaCl) and 540 (100 mM NaCl) differentially abundant proteins (DAPs) were screened out. Compared with the control, in the 50 mM NaCl solution, 61 and 82 DAPs were upregulated and downregulated, respectively, and in the 100 mM NaCl solution, 222 and 318 DAPs were upregulated and downregulated, respectively. Furthermore, 113 DAPs were copresent in the 50 mM and 100 mM NaCl treatments, of which 43 were upregulated and 70 were downregulated. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the DAPs induced by salt stress during mulberry seed germination were mainly involved in photosynthesis, carotenoid biosynthesis and phytohormone signaling. Finally, PRM verified five differentially expressed proteins, which demonstrated the reliability of TMT in analyzing protein groups. Conclusions: Our research provides valuable insights to further study the overall mechanism of salt stress responses and salt tolerance of mulberry and other plants.

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Differential proteomics: Effect of growth regulators on salt stress responses in safflower seedlings

Cited by 6 publications

References 33 publications

The transcriptional regulatory network of hormones and genes under salt stress in tomato plants (Solanum lycopersicum L.)

The transcriptional regulatory network of hormones and genes under salt stress in tomato plants (Solanum lycopersicum L.)

Paraphoma chrysanthemicola Affects the Carbohydrate and Lobetyolin Metabolism Regulated by Salicylic Acid in the Soilless Cultivation of Codonopsis pilosula

Physiological and Proteomic Analysis of Seed Germination under Salt Stress in Mulberry

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