Differential proteomics analysis of serum exosomein burn patients

Abstract

The exosome is an emerging concepts biomarkers due to their abnormal expression in various diseases. Research on exosome has already shifted from the laboratory to clinical application. This study uses bioinformatics technology to identify functional changes in proteins of serum exosomes from burn patients. A total of 231 quantifiable differentially-expressed proteins were screened out, 31 of them had statistically significant changes in expression levels. In the test group, expression of2 proteins had downregulated, whereas that of 29 proteins upregulated. Gene Ontology analysis demonstrates that differentially-expressed proteins were primarily identified in extracellular vesicles and platelet α granules, which can alter enzyme inhibitor activities, heparin-binding, coagulation, and lipid transport. Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrates that ITGA2B and ITGB3 proteins, which were significantly upregulated in the burn group, were primarily involved in the PI3K/AKT signaling pathway. Western blotting confirmed that the expressions of ITGA2B and ITGB3 in burn patient tissue samples were higher than those in the control group; conversely, the expression of CD9 was lower than that in the control group. In burn patients, the upregulated proteins ITGA2B and ITGB3 of serum exosomes likely participate in injury detection and repair via PI3K/AKT signaling pathways.

Kukoamine A Improves Mycoplasma pneumoniae Pneumonia by Regulating miR-222-3p/Superoxide Dismutase 2

“…Clinical Specimens. Patients' blood was prepared to collect exosomes as recently described [25]. All serum specimens (MPP patients: n = 10; healthy: n = 10) were stored at -80 °C and further used for exosomes isolation.…”

“…Isolation and Identification of Serum Exosomes. This procedure was performed as recently described [25]. Briefly, serum exosomes were individually extracted from the healthy (n = 10) and patients (n = 10) with MPP with 10,000 × g for 30 min at 4 °C.…”

Multimerin-1 and cancer: a review

“…In addition to cancer, MMRN1 is differentially expressed in other diseases including inflammation and bacterial and viral infections (186,(194)(195)(196)(197). Upregulation of MMRN1 has also been observed in injuries including septic shock-associated kidney injury (198) and in serum exosomes from burn patients (185). MMRN1 may also play a role in human pathogen interactions.…”

“…Exosomes are able to promote metastasis [ 184 ], but how MMRN1 exosomal release is associated with cancer progression has not been investigated yet. Elevation of MMRN1 levels in serum exosomes has also been observed in burn patients [ 185 ] and patients with tuberculosis infection [ 186 ].…”

Alarming Cargo: The Role of Exosomes in Trauma-Induced Inflammation

“…Qin et al found that there was differential expression of 31 proteins in the serum exosomes of burn patients. Of particular note in their findings was the upregulation of ITGA2B and ITGB3 in the burn group, as these proteins act in the PI3K/AKT signaling pathway, which promotes cell proliferation and growth [ 253 ]. Wound healing, especially cutaneous, is critical in a burn patient, to restore the body’s barrier to fluid loss and infection.…”