Differential proteomic profiling of endometrium and plasma indicate the importance of hydrolysis in bovine endometritis

Zhang, Shidong; Dong, Shuwei; Wang, Dongsheng; Oguejiofor, Chike F.; Fouladi-Nashta, Ali A.; Yang, Zhiqiang; Yan, Zuoting

doi:10.3168/jds.2016-12365

Cited by 15 publications

(14 citation statements)

References 74 publications

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“…Zhang et al . 12 used iTRAQ technology to screen differential proteins between the endometrium and plasma of cows with endometritis. In view of the literature and our previous studies, we speculate that certain proteins in the blood of cows may change at 60–90 days postpartum, but to date, these proteins have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus

Sheng

Bai

Wang

et al. 2019

Sci Rep

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To screen differentially expressed proteins in the blood dairy cows with inactive ovaries caused by a negative energy balance and to determine the roles of the identified proteins in the development of inactive ovaries.Holstein cows at 14 to 21 days postpartum in an intensive dairy farm were examined for their energy balance (EB) status by blood β-hydroxybutyrate (BHBA) and assigned to the inactive ovary (IO) group (n = 50) and the normal oestrus control (CON) group (n = 50) at 60 to 90 days postpartum by means of the oestrus manifestation, rectal examination and B-ultrasound examination. Fourteen differentially expressed proteins from 61 proteins in the plasma of dairy cows with IOs were identified by iTRAQ/LC-MS/MS and GO, KEGG, and PATHWAY analysis. Eleven expressed proteins were upregulated, and 3 expressed proteins were downregulated. Among the 10 differentially expressed proteins verified by Western blot or ELISA, the relative expression levels of ALDOB, IGFBP2, ITIH3 and LDHB in mixed samples and single samples were consistent with the proteomic protein results. PKM2, GPX3, ALDOB, RBP4 and AHSG were significantly different between the two groups (P < 0.05); APOA4 and SPAM1 were not significantly different (P > 0.05) but were still downregulated in the ovarian resting group. This study confirmed that 14 plasma differential proteins in the inactive ovaries of postpartum dairy cows were associated with follicular development, and these findings provide a foundation for further research on the mechanism and prevention of inactive ovaries in dairy cows.

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Section: Introductionmentioning

confidence: 99%

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus

Sheng

Bai

Wang

et al. 2019

Sci Rep

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“…Conjunctly, they highlight the importance of inflammation-related proteins, especially acute phase proteins; among those are heat shock proteins (HSP) and Major Histocompatibility Complex 1 (MHC1) (Choe et al 2010) as well as cathelicidins, of antimicrobial function (Kosciuczuk et al 2012). Zhang et al (2017) found five (cathelicidins 2, 3, 5-7) to be significantly more abundant in endometritic tissue. Ledgard et al (2015) found cathelicidins highly correlated with the percentage of polymorphonuclear leukocytes in UF affected by subclinical endometritis, indicating their production by these immune cells.…”

Section: Endometritismentioning

confidence: 94%

“…Although to a lesser extent, endometrial tissue is also liable to the same issue (Berendt et al 2005). In this regard, Zhang et al (2017) found proteins of the blood complement system in endometrial samples which they attributed to plasma contamination, possibly as a result of haemorrhage.…”

Section: Sampling or Chemical Processingmentioning

confidence: 97%

“…The molecular characterisation of local response to infection in reproductive tissues provides an extreme example of detrimental conditions for fertility to which milder conditions might be related to. Four studies investigating clinical (Choe et al 2010, Zhang et al 2017) and subclinical (Ledgard et al 2015, Helfrich et al 2020 endometritis have been surveyed in this systematic review. Conjunctly, they highlight the importance of inflammation-related proteins, especially acute phase proteins; among those are heat shock proteins (HSP) and Major Histocompatibility Complex 1 (MHC1) (Choe et al 2010) as well as cathelicidins, of antimicrobial function (Kosciuczuk et al 2012).…”

Section: Endometritismentioning

confidence: 99%

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Proteomics and metabolomics in cow fertility: a systematic review

et al. 2020

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Cow subfertility is a multi-factorial problem in many countries which is only starting to be unravelled. Molecular biology can provide a substantial source of insight into its causes and potential solutions, particularly through large scale, untargeted omics approaches. In this systematic review we set out to compile, assess and integrate the latest proteomic and metabolomic research on cow reproduction, specifically that on the female reproductive tract and early embryo. We herein report a general improvement in technical standards throughout the temporal span examined, however significant methodological limitations are also identified. We propose easily actionable avenues for ameliorating these shortcomings and enhancing the reach of this field. Text mining and pathway analysis corroborate the relevance of proteins and metabolites related to the triad oxidative stress-inflammation-disease on reproductive function. We envisage a breakthrough in cattle reproductive molecular research within the next few years as in vivo sample techniques are improved, omics analysis equipment becomes more affordable and widespread, and software tools for single- and multi-omics data processing are further developed. Additional investigation of the impact of local oxidative stress and inflammation on fertility, both at the local and systemic levels, is key towards realising the full potential of this field.

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“…Proteomics studies performed in tissues and various body fluids of dairy cows during the transition period were reviewed by Ceciliani et al (2018). The proteome in blood plasma has been described in context with hypocalcemia (Xia et al, 2012;Fan et al, 2017) and endometritis (Zhang et al, 2017;Miller et al, 2019) and during intramammary infection (Kim et al, 2011). Other proteomics studies have advanced understanding of the liver proteome of cows under heat stress (Skibiel et al, 2018) and the adipose tissue (AT) proteome of cows during the postpartum period (Takiya et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Plasma proteomic profiling and pathway analysis of normal and overconditioned dairy cows during the transition from late pregnancy to early lactation

Ghaffari

Schuh

Kuleš

et al. 2020

Journal of Dairy Science

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This study applied a quantitative proteomics approach along with bioinformatics analyses to investigate changes in the plasma proteome of normal and overconditioned dairy cows during the transition period. Fifteen weeks before their anticipated calving date, 38 multiparous Holstein cows were selected based on their current and previous body condition scores (BCS) and allocated to either a high or a normal BCS group (19 cows each). They received different diets until dry-off to reach targeted differences in BCS and back fat thickness (BFT) until dry-off. At dry-off, normal BCS cows had a BCS <3.5 (minimum, 2.75) and BFT <1.2 cm (minimum, 0.58), and the high BCS cows had a BCS >3.75 (maximum, 4.50) and BFT >1.4 cm (maximum, 2.90). The proteomics study used a subset of 5 animals from each group. These cows were selected based on their circulating concentrations of fatty acids (FA) on d 14 postpartum and β-hydroxybutyrate (BHB) on d 21 postpartum, representing the greater or the lower extreme values within their BCS group, respectively. The high BCS subset (HE-HBCS) had 4.50 < BCS > 3.75, FA = 1.17 ± 0.46 mmol/L, and BHB = 2.15 ± 0.42 mmol/L (means ± SD), and the low BCS subset (LE-NBCS) had 3.50 < BCS > 2.75, FA = 0.51 ± 0.28 mmol/L, and BHB = 0.84 ± 0.17 mmol/L. Plasma samples from d −49, +7, and +21 relative to parturition were used for proteome profiling by applying the quantitative tandem mass tags (TMT) approach. Nondepleted plasma samples were subjected to reduction and digestion and then labeled with TMT 10plex reagents. High-resolution liquid chromatography-tandem mass spectrometry analysis of TMT-labeled peptides was carried out, and the acquired spectra were analyzed for protein identification and quantification. In total, 254 quantifiable proteins (criteria: 2 unique peptides and 5% false discovery rate) were identified in the plasma samples. From these, 24 differentially abundant proteins (14 more abundant, 10 less abundant) were observed in the LE-NBCS cows compared with the HE-HBCS cows during the transition period. Plasma α-2-macroglobulins were more abundant in HE-HBCS versus LE-NBCS cows at d +7 and +21. Gene Ontology enrichment analyses of differentially abundant proteins revealed that the acute inflammatory response, regulation of complement activation, protein activation cascade, and regulation of humoral immune response were the most enriched terms in the LE-NBCS group compared with the HE-HBCS group. In addition, we identified 24 differentially abundant proteins (16 in the LE-NBCS group, and 8 in the HE-HBCS group) during the transition period. The complement components C1q and C5 were less abundant, while C3 and C3d were more abundant in LE-NBCS compared with HE-HBCS cows. Overall, overconditioning around calving was associated with alterations in protein pathways related to acute inflammatory response and regulation of complement and coagulation cascades in transition cows.

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Differential proteomic profiling of endometrium and plasma indicate the importance of hydrolysis in bovine endometritis

Cited by 15 publications

References 74 publications

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus

Proteomics and metabolomics in cow fertility: a systematic review

Plasma proteomic profiling and pathway analysis of normal and overconditioned dairy cows during the transition from late pregnancy to early lactation

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