Differential proteomic analysis to identify proteins associated with Tenderness of Yak meat from different parts based on TMT Proteomic

Abstract

In order to study the tenderness mechanism of difference in different parts of Yak meat, the differentially expressed proteins (DEPs) and its correlation with quality of Yak meat were studied by TMT (Tandem Mass Tags, TMT) quantitative proteomic method. The results showed that 25, 40 and 34 DEPs were identified in JR/HGT, WJR/HGT and WJR/JR groups, respectively. Correlation analysis between DEPs and quality traits of yak meat showed that there were 12 DEPs with significant correlation were correlated closely with quality traits.The relative contents of the 11 DEPs were significantly different in different parts (p < 0.05).There are 8, 9 and 6 key DEPs in JR/HGT, WJR/HGT and WJR/JR groups, respectively.The expression of these DEPs were down-regulated and up-regulated in different parts, and was closely related to the cell growth and death, carbohydrate metabolism, amino acid metabolism, protein binding, motility activity and unfolded protein binding.The significant difference of the expression of these key proteins in different parts is the internal reason of the difference of quality traits among WJR, JR and HGT.This provide a reference for the study on the mechanism of difference in Yak meat.

Correlation analysis between soluble sugars of soybean and mucus drawing length of fermented natto

“…Data are expressed as the mean ± standard deviation and were obtained from three independent experiments. All calculations were implemented using SPSS 20.0 software, the data were statistically analyzed by Pearson's correlation coefficient test and one-way ANOVA plus post hoc Duncan's multiple-comparison test, p < 0.05 was defined as statistical significance, and Origin 9.0 was used in drawing (Huang et al, 2022;Yan et al, 2021).…”