Differential Proteomic Analysis of Subfractioned Human Hepatocellular Carcinoma Tissues

Codarin, Erika; Renzone, Giovanni; Poz, Alessandra; Avellini, Claudio; Baccarani, Umberto; Lupo, Francesco; Maso, Vittorio Di; Croce, Saveria; Tiribelli, Claudio; Arena, Simona; Quadrifoglio, Franco; Scaloni, Andrea; Tell, Gianluca

doi:10.1021/pr8009275

Cited by 14 publications

(12 citation statements)

References 118 publications

(187 reference statements)

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“…at sub‐pathological doses, is of particular importance. It is a current procedure allowing for the identification of biomarkers 10–15 and making possible human studies that otherwise could not be carried out at overtly toxic exposures 16.…”

Section: Environmental Proteomicsmentioning

confidence: 99%

Proteins in ecotoxicology – How, why and why not?

2010

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The growing interest in the application of proteomic technologies to solve toxicology issues and its relevance in ecotoxicology research has resulted in the emergence of "ecotoxicoproteomics". There is a general consensus that ecotoxicoproteomics is a powerful tool to spot early molecular events involved in toxicant responses, which are responsible for the adverse effects observed at higher levels of biological organization, thus contributing to elucidate the mode of action of stressors and to identify specific biomarkers. Ultimately, early-warning indicators can then be developed and deployed in "in situ" bioassays and in environmental risk assessment. The number of field experiments or laboratory trials using ecologically relevant test-species and involving proteomics has been, until recently, insufficient to allow a critical analysis of the real benefits of the application of this approach to ecotoxicology. This article intends to present an overview on the applications of proteomics in the context of ecotoxicology, focusing mainly on the prospective research to be done in invertebrates. Although these represent around 95% of all animal species and in spite of the key structural and functional roles they play in ecosystems, proteomic research in invertebrates is still in an incipient stage. We will review applications of ecotoxicoproteomics by evaluating the technical methods employed, the organisms and the contexts studied, the advances achieved until now and lastly the limitations yet to overcome will be discussed.

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Section: Environmental Proteomicsmentioning

confidence: 99%

Proteins in ecotoxicology – How, why and why not?

2010

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“…a-Fetoprotein (AFP), as the most widely used tumor marker in clinical practice, is not an effective marker for hepatocellular carcinoma diagnosis due to its poor sensitivity and low specificity (4)(5)(6). To explore more sensitive and specific markers for early and accurate diagnosis of hepatocellular carcinoma, there have been a number of previous investigations on gene expression, miRNA profiles, and protein expression of hepatocellular carcinoma (7)(8)(9)(10)(11)(12). Recently, metabolomics investigations have provided a new angle for biomarker discovery.…”

Section: Introductionmentioning

confidence: 99%

Acetylcarnitine Is a Candidate Diagnostic and Prognostic Biomarker of Hepatocellular Carcinoma

Gao

et al. 2016

Cancer Research

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The identification of serum biomarkers to improve the diagnosis and prognosis of hepatocellular carcinoma has been elusive to date. In this study, we took a mass spectroscopic approach to characterize metabolic features of the liver in hepatocellular carcinoma patients to discover more sensitive and specific biomarkers for diagnosis and progression. Global metabolic profiling of 50 pairs of matched liver tissue samples from hepatocellular carcinoma patients was performed. A series of 62 metabolites were found to be altered significantly in liver tumors; however, levels of acetylcarnitine correlated most strongly with tumor grade and could discriminate between hepatocellular carcinoma tumors and matched normal tissues. Post hoc analysis to evaluate serum diagnosis and progression potential further confirmed the diagnostic capability of serum acetylcarnitine. Finally, an external validation in an independent batch of 58 serum samples (18 hepatocellular carcinoma patients, 20 liver cirrhosis patients, and 20 healthy individuals) verified that serum acetylcarnitine was a meaningful biomarker reflecting hepatocellular carcinoma diagnosis and progression. These findings present a strong new candidate biomarker for hepatocellular carcinoma with potentially significant diagnostic and prognostic capabilities.

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“…HCC pathogenesis is quite complicated and is basically triggered by chromosomal aberrations, epigenetic abnormalities and changes in gene expression. At the molecular level, the onset and progression of HCC has been observed to be mechanistically linked to the differential expression of genes (Feitelson et al, ; Lee & Thorgeirsson, ; Lleonart et al, ; Wang et al, ; Sharma, Vouros, & Glick, ), deregulation of protein expression (Cui et al, ; Codarin et al, ; Corona et al, ; D'Alessandro, Meyer, & Klingmuller, ), particular RNA modifications (Santamaría et al, ; Yang et al, ) and, markedly, metabolic perturbations (Xue et al, ; Patterson et al, ; Budhu et al, ; Wang et al, ) (Fig. ).…”

Section: Assignments Of Omics Technologies To Hcc Pathogenesismentioning

confidence: 99%

“…Mass spectrometry‐based proteomics strategy is capable of identifying the differentially expressed proteins from a large validated protein database, that together. The combination of with genetic/transcriptomic/metabolomics and bioinformatics identification and integration of these multiple ‘omics can determine the functional significance of proteins and biochemical pathways responsible for as HCC pathogenesis, as well as other diseases (Cui et al, ; Codarin et al, ; Gstaiger & Aebersold, ; Corona et al, ; Lee et al, ).…”

Section: Proteomic Snapshot Of the Molecular Signature Of Hccmentioning

confidence: 99%

Advanced mass spectrometry‐based multi‐omics technologies for exploring the pathogenesis of hepatocellular carcinoma

Nie

Yan

Lee

et al. 2014

Mass Spectrometry Reviews

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Hepatocellular carcinoma (HCC) is one of the primary hepatic malignancies and is the third most common cause of cancer related death worldwide. Although a wealth of knowledge has been gained concerning the initiation and progression of HCC over the last half century, efforts to improve our understanding of its pathogenesis at a molecular level are still greatly needed, to enable clinicians to enhance the standards of the current diagnosis and treatment of HCC. In the post‐genome era, advanced mass spectrometry driven multi‐omics technologies (e.g., profiling of DNA damage adducts, RNA modification profiling, proteomics, and metabolomics) stand at the interface between chemistry and biology, and have yielded valuable outcomes from the study of a diversity of complicated diseases. Particularly, these technologies are being broadly used to dissect various biological aspects of HCC with the purpose of biomarker discovery, interrogating pathogenesis as well as for therapeutic discovery. This proof of knowledge‐based critical review aims at exploring the selected applications of those defined omics technologies in the HCC niche with an emphasis on translational applications driven by advanced mass spectrometry, toward the specific clinical use for HCC patients. This approach will enable the biomedical community, through both basic research and the clinical sciences, to enhance the applicability of mass spectrometry‐based omics technologies in dissecting the pathogenesis of HCC and could lead to novel therapeutic discoveries for HCC. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:331–349, 2016.

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Differential Proteomic Analysis of Subfractioned Human Hepatocellular Carcinoma Tissues

Cited by 14 publications

References 118 publications

Proteins in ecotoxicology – How, why and why not?

Proteins in ecotoxicology – How, why and why not?

Acetylcarnitine Is a Candidate Diagnostic and Prognostic Biomarker of Hepatocellular Carcinoma

Advanced mass spectrometry‐based multi‐omics technologies for exploring the pathogenesis of hepatocellular carcinoma

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