Differential Proteomic Analysis of Human Sperm: A Systematic Review to Identify Candidate Targets to Monitor Sperm Quality

Abstract

Purpose The advent of proteomics provides new opportunities to investigate the molecular mechanisms underlying male infertility. The selection of relevant targets based on a single analysis is not always feasible, due to the growing number of proteomic studies with conflicting results. Thus, this study aimed to systematically review investigations comparing the sperm proteome of normozoospermic and infertile men to define a panel of proteins with the potential to be used to evaluate sperm quality. Materials and Methods A literature search was conducted on PubMed, Web of Science, and Scopus databases following the PRISMA guidelines. To identify proteins systematically reported, first the studies were divided by condition into four groups (asthenozoospermia, low motility, unexplained infertility, and infertility related to risk factors) and then, all studies were analysed simultaneously (poor sperm quality). To gain molecular insights regarding identified proteins, additional searches were performed within the Human Protein Atlas, Mouse Genome Informatics, UniProt, and PubMed databases. Results Thirty-two studies were included and divided into 4 sub-analysis groups. A total of 2752 proteins were collected, of which 38, 1, 3 and 2 were indicated as potential markers for asthenozoospermia, low motility, unexplained infertility and infertility related to risk factors, respectively, and 58 for poor sperm quality. Among the identified proteins, ACR, ACRBP, ACRV1, ACTL9, AKAP4, ATG3, CCT2, CFAP276, CFAP52, FAM209A, GGH, HPRT1, LYZL4, PRDX6, PRSS37, REEP6, ROPN1B, SPACA3, SOD1, SPEM1, SPESP1, SPINK2, TEKT5, and ZPBP were highlighted due to their roles in male reproductive tissues, association with infertility phenotypes or participation in specific biological functions in spermatozoa. Conclusions Sperm proteomics allows the identification of protein markers with the potential to overcome limitations in male infertility diagnosis and to understand changes in sperm function at the molecular level. This study provides a reliable list of systematically reported proteins that could be potential targets for further basic and clinical studies.

The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening

“…It has been suggested that the expression of genes encoding proteins related to fertilization may serve as markers for predicting male fertility [33]. Table 1 lists some testis-specific proteins that are considered candidates towards a marker panel for male fertility impairment [34,35]. Among the proteins secreted in the male reproductive system, we identified acrosin (P10323, ACR), which is important in the merger between sperm and egg.…”

“…Total acrosin activity may be considered a sensitive biochemical marker for the clinical evaluation of unexplained infertility in males [36]. In addition to ACR, the zona pellucida binding protein (Q9BS86, ZPBP) and A-kinase anchor protein 4 (Q5JQC9, AKAP4) were also highlighted due to their role in male reproductive tissues, association with infertility phenotypes, and participation in specific biological functions in spermatozoa [35]. AKAP4 plays a crucial role in maintaining the integrity of sperm fibrous sheaths and normal sperm motility.…”