Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery

Abstract

BackgroundHigh temperature is a major environmental factor limiting grape yield and affecting berry quality. Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of the thermotolerance of Vitis, we combined a physiological analysis with iTRAQ-based proteomics of Vitis vinifera cv Cabernet Sauvignon, subjected to 43°C for 6 h, and then followed by recovery at 25/18°C.ResultsHigh temperature increased the concentrations of TBARS and inhibited electronic transport in photosynthesis apparatus, indicating that grape leaves were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. One hundred and seventy-four proteins were differentially expressed under heat stress and/or during the recovery phase, in comparison to unstressed controls, respectively. Stress and recovery conditions shared 42 proteins, while 113 and 103 proteins were respectively identified under heat stress and recovery conditions alone. Based on MapMan ontology, functional categories for these dysregulated proteins included mainly photosynthesis (about 20%), proteins (13%), and stress (8%). The subcellular localization using TargetP showed most proteins were located in the chloroplasts (34%), secretory pathways (8%) and mitochondrion (3%).ConclusionOn the basis of these findings, we proposed that some proteins related to electron transport chain of photosynthesis, antioxidant enzymes, HSPs and other stress response proteins, and glycolysis may play key roles in enhancing grapevine adaptation to and recovery capacity from heat stress. These results provide a better understanding of the proteins involved in, and mechanisms of thermotolerance in grapevines.

Protein sHSP26 improves chloroplast performance under heat stress by interacting with specific chloroplast proteins in maize (Zea mays)

“…Besides, the abundance of PSI reaction center subunit VI was increased by heat stress. Previous studies indicated that PSI reaction center subunits III, N and VI were up-regulated by heat stress [27]. Our results here showed that PSI reaction center subunit VI interacted with sHSP26 and RNAimediated silencing of sHSP26 reduced the accumulation of oxygen-evolving enhancer protein 1.…”

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

“…Wu et al (2015) investigated changes of fruit quality and protein expression profiles of grape berries upon hot water treatment during the subsequent 45 d of cold storage, finding immediate increased expression of proteins associated with carbohydrate and energy metabolism. Liu et al (2014) combined a physiological analysis with iTRAQ-based proteomics of cv Cabernet Sauvignon grape leaves subjected to 43°C stress for 6 h. They proposed that some proteins related to the electron transport chain of photosynthesis, antioxidant enzymes, HSPs, and other stress response proteins, as well as glycolysis, may play key roles in enhancing grapevine adaptation to and recovery capacity from heat stress. However, these transcriptomic and proteomic studies were not conducted on the same grape materials, so it is difficult to compare their results.…”

Comparative proteomic analysis reveals the positive effect of exogenous spermidine on photosynthesis and salinity tolerance in cucumber seedlings

“…Total protein was extracted using the cold acetone method (Liu et al 2014). Two biological replicates were carried out for each sample.…”

“…Among those proteins, 221 proteins were characterized as differentially expressed proteins, with at least two significant peptide sequences and an expression ratio[1.50 or\0.67 (Liu et al 2014;Wang et al 2013) under exogenous Spd or NaCl compared with the control and in Spd?NaCl-treated plants compared with NaCl alone (Supporting Information Table 1 and 2). Compared with the control, exogenous Spd treatment changed the abundances of 45 proteins: 40 proteins were increased and five were decreased.…”

“…To further analyze the differentially expressed proteins in leaves caused by salt and/or Spd treatment, the identified proteins were grouped into 30 functional categories using the Mercator (MapMan) ontology (Fan et al 2011;Liu et al 2014;Lohse et al 2014), as shown in Fig. 3b.…”