Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Pinheiro, Tânia; Lip, Ka Ying Florence; García‐Ríos, Estéfani; Querol, Amparo; Teixeira, J. A.; Gulik, Walter van; Guillamón, José Manuel; Domingues, Lucı́lia

doi:10.1038/s41598-020-77846-w

Cited by 23 publications

(16 citation statements)

References 89 publications

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“…Ethanol Red response to this supraoptimal temperature was found to trigger mechanisms like repression of proteins involved in arginine biosynthesis (Arg1, Arg5, Arg6, and Arg8) and induction of a protein (Car2). The latter catalyzes arginine degradation, guaranteeing glutamate conservation and proline production, which is thought to be involved in stress protection . This, though, does not occur in CEN.PK113-7D .…”

Section: Resultsmentioning

confidence: 68%

Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains

et al. 2021

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Resveratrol is a phenolic compound with strong antioxidant activity, being promising for several applications in health, food, and cosmetics. It is generally extracted from plants or chemically synthesized, in both complex and not sustainable processes, but microbial biosynthesis of resveratrol can counter these drawbacks. In this work, resveratrol production by microbial biosynthesis from lignocellulosic materials was assessed. Three robust industrial Saccharomyces cerevisiae strains known for their thermotolerance and/or resistance to inhibitory compounds were identified as suitable hosts for de novo resveratrol production from glucose and ethanol. Through the CRISPR/Cas9 system, all industrial strains, and a laboratory one, were successfully engineered with the resveratrol biosynthetic pathway via the phenylalanine intermediate. All strains were further screened at 30 °C and 39 °C to evaluate thermotolerance, which is a key feature for Simultaneous Saccharification and Fermentation processes. Ethanol Red RBP showed the best performance at 39 °C, with more than 2.6-fold of resveratrol production in comparison with the other strains. This strain was then used to assess resveratrol production from glucose and ethanol. A maximum resveratrol titer of 187.07 ± 19.88 mg/L was attained from a medium with 2% glucose and 5% ethanol (w/v). Lastly, Ethanol Red RBP produced 151.65 ± 3.84 mg/L resveratrol from 2.95% of cellulose from hydrothermally pretreated Eucalyptus globulus wood, at 39 °C, in a Simultaneous Saccharification and Fermentation process. To the best of our knowledge, this is the first report of lignocellulosic resveratrol production, establishing grounds for the implementation of an integrated lignocellulose-to-resveratrol process in an industrial context.

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Section: Resultsmentioning

confidence: 68%

Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains

et al. 2021

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“…Additionally, the thermotolerant industrial strains presented significantly higher trehalose accumulation compared to the reference laboratorial CEN.PK113-7D strain. At the proteome level, temperature responses also differ between S. cerevisiae strains [30]. Interestingly, thermotolerant S. cerevisiae Ethanol Red response to both supra and sub-optimal temperature involved the overexpression of Erg13-a protein involved in early ergosterol biosynthesis-and Gsy1-a glycogen synthetase [30].…”

Section: Robustness Of the Fermentation Organismmentioning

confidence: 99%

Very High Gravity Bioethanol Revisited: Main Challenges and Advances

et al. 2021

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Over the last decades, the constant growth of the world-wide industry has been leading to more and more concerns with its direct impact on greenhouse gas (GHG) emissions. Resulting from that, rising efforts have been dedicated to a global transition from an oil-based industry to cleaner biotechnological processes. A specific example refers to the production of bioethanol to substitute the traditional transportation fuels. Bioethanol has been produced for decades now, mainly from energy crops, but more recently, also from lignocellulosic materials. Aiming to improve process economics, the fermentation of very high gravity (VHG) mediums has for long received considerable attention. Nowadays, with the growth of multi-waste valorization frameworks, VHG fermentation could be crucial for bioeconomy development. However, numerous obstacles remain. This work initially presents the main aspects of a VHG process, giving then special emphasis to some of the most important factors that traditionally affect the fermentation organism, such as nutrients depletion, osmotic stress, and ethanol toxicity. Afterwards, some factors that could possibly enable critical improvements in the future on VHG technologies are discussed. Special attention was given to the potential of the development of new fermentation organisms, nutritionally complete culture media, but also on alternative process conditions and configurations.

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“…Recently, the differential proteomic responses of three distinct S. cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- and supra-optimal temperatures were studied under chemostat conditions, mimicking the industrial processes [ 29 , 32 ]. The proteomic profile of these strains was performed by SWATH-MS, allowing the quantification of 997 proteins [ 32 ]. Overall, proteomic data evidenced that at high temperature (39°C), the amino acid biosynthetic pathways and metabolism represent the main function recruited [ 32 ].…”

Section: Improvement Of Process-related Traitsmentioning

confidence: 99%

“…The proteomic profile of these strains was performed by SWATH-MS, allowing the quantification of 997 proteins [ 32 ]. Overall, proteomic data evidenced that at high temperature (39°C), the amino acid biosynthetic pathways and metabolism represent the main function recruited [ 32 ]. The variability of responses of the three strains examined showed that no general rules can be assumed for different S. cerevisiae strains, and that the temperature-response is highly dependent on their genetic and environmental background [ 32 ].…”

Section: Improvement Of Process-related Traitsmentioning

confidence: 99%

“…Overall, proteomic data evidenced that at high temperature (39°C), the amino acid biosynthetic pathways and metabolism represent the main function recruited [ 32 ]. The variability of responses of the three strains examined showed that no general rules can be assumed for different S. cerevisiae strains, and that the temperature-response is highly dependent on their genetic and environmental background [ 32 ]. At 39°C, the best performing strain at supra-optimal temperatures, increased the expression of proteins involved in ergosterol and glycogen synthesis, along with Hsp104p, which are known to play a crucial role in heat adaptation [ 32 ].…”

Section: Improvement Of Process-related Traitsmentioning

confidence: 99%

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Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

et al. 2020

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The biorefinery concept, consisting in using renewable biomass with economical and energy goals, appeared in response to the ongoing exhaustion of fossil reserves. Bioethanol is the most prominent biofuel and has been considered one of the top chemicals to be obtained from biomass. Saccharomyces cerevisiae, the preferred microorganism for ethanol production, has been the target of extensive genetic modifications to improve the production of this alcohol from renewable biomasses. Additionally, S. cerevisiae strains from harsh industrial environments have been exploited due to their robust traits and improved fermentative capacity. Nevertheless, there is still not an optimized strain capable of turning second generation bioprocesses economically viable. Considering this, and aiming to facilitate and guide the future development of effective S. cerevisiae strains, this work reviews genetic engineering strategies envisioning improvements in 2 nd generation bioethanol production, with special focus in process-related traits, xylose consumption, and consolidated bioprocessing. Altogether, the genetic toolbox described proves S. cerevisiae to be a key microorganism for the establishment of a bioeconomy, not only for the production of lignocellulosic bioethanol, but also having potential as a cell factory platform for overall valorization of renewable biomasses.

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Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Cited by 23 publications

References 89 publications

Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains

Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains

Very High Gravity Bioethanol Revisited: Main Challenges and Advances

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

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