Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Abstract

Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, EthanolRed, grown at sub-and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains was performed by SWATH-MS, allowing the quantification of 997 proteins, data available via ProteomeXchange (PXD016567). Our analysis demonstrated that temperature responses differ between the strains; however, we also found some common responsive proteins, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome. The proteomic data evidenced that the cold response involves strong repression of translation-related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation while, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects the yeast proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance.

Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrialSaccharomyces cerevisiaeas efficient whole cell biocatalysts

“…2B and 2C, Table 2), being observable a clear accumulation of xylose at 24 hours of fermentation, result of the low fermentative aptitude of these strains in comparison with their xylan-degrading capacity. This superior performance of ER-X-2P is in accordance with previous reports of Ethanol Red excellent fermentation capacity, robustness, stress [33] and temperature tolerance [34]. In fact, Ethanol Red has recently been successively used as host for consolidated bioprocessing (CBP) for first generation bioethanol using raw starch [1], with the present work showing this strain applicability also for a more challenging CBP for second generation hemicellulosic ethanol.…”

Co-production of biofuels and value-added compounds from industrial Eucalyptus globulus bark residues using hydrothermal treatment

“…The strain employed in this study was the industrial Saccharomyces cerevisiae Ethanol-Red®, commonly reported for its efficiency at high temperatures [34,35]. For inoculum preparation, 2-3 colonies (from stock cultures kept on YPD agar plates) were transferred into 250 mL Erlenmeyer flask, containing 100 mL of YPD medium (50 g/L glucose, 20 g/L peptone and 10 g/L yeast extract) and then incubated at 30 • C and 150 rpm for 18 h. The cells were aseptically collected by centrifugation (10 min; 4000 g) and resuspended in 0.9% NaCl to a final concentration of 200 mg fresh yeast/mL.…”

Selection and subsequent physiological characterisation of industrialSaccharomyces cerevisiaestrains during continuous growth at sub- and- supra optimal temperatures

“…Another cause of the effect of temperature on Y ATP could be protein misfolding at high temperatures and aggregation at low temperatures. Remarkably the proteome analysis of the identical chemostat cultured strains revealed that for both CEN.PK113-7D and ADY5 the proteins related to protein folding and degradation processes were upregulated at 12 °C, in contrast to Ethanol Red [33]. This could indicate that protein aggregation and/or misfolding and subsequent degradation and re-synthesis might have occurred in these strains at 12 °C, resulting in an increased energy demand and thus a decreased Y ATP .…”

“…All three strains showed upregulation of proteins involved in transport and metabolism of carbohydrates as well as energy and amino acid metabolism at 12 °C compared to 30 °C [33]. This shows that maintaining the same specific growth rate of 0.03 h -1 in the chemostat at 12°C, where maximum enzyme capacities have decreased, requires upregulation of proteins in central metabolism.…”