Differential Proteins of the Optic Ganglion in Octopus vulgaris Under Methanol Stress Revealed Using Proteomics

Abstract

An analytical approach using the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique separated the proteome from the optic ganglia of Octopus vulgaris (OVOG). Approximately 600 protein spots were detected from the extraction when applying 150 μg protein to a 2D-PAGE gel in the pH range 5.0-8.0. Compared to the control, significant changes of 18 protein spots were observed in OVOG under the stress of native seawater containing 2% methanol for 72 h. Among these spots, we found that eight were down-regulated and ten were up-regulated in the gels, which were further identified using both peptide mass fingerprinting and database searches. Significant proteins such as glyceraldehyde-3-phosphate dehydrogenase, alpha subunit of succinyl-CoA synthetase, alcohol dehydrogenase, and long-chain specific acyl-CoA dehydrogenase were up-regulated proteins, whereas putative ABC transporter was a down -regulated protein. These differential proteins at the level of subcellular localization were further classified using LOCtree software with a hierarchical system of support vector machines. We found that most of the differential proteins in the gel could be identified as mitochondrial proteins, suggesting that these protective or marker proteins might help to prevent methanol poisoning via the mitochondria in the optical ganglia. The results indicated that both beta-tubulin and beta-actin were potential biomarkers as up-regulated proteins for monitoring methanol toxicosis associated with fish foods such as octopus and shark.

Construction of nanometer cisplatin core-ferritin (NCC-F) and proteomic analysis of gastric cancer cell apoptosis induced with cisplatin released from the NCC-F

“…7 and 8). Based on our knowledge and experience in proteomics, the biomarkers for monitoring should be considered as a group of differential proteins consisting of high frequency and individual proteins rather than single proteins [45,46]. Here, we indicated that the major apoptosis pathway of various cancer cells (including tumors) induced in vivo with CDDP were closely connected with the differential proteins shown in both Figs.…”

Identifying Natural Bioactive Peptides from the Common Octopus (Octopus vulgaris Cuvier, 1797) Skin Mucus By-Products Using Proteogenomic Analysis

“…The common octopus is a species with great food importance, since it is a complete food with great nutritional value and it is highly appreciated. Due to the high scientific interest and high commercial value of the common octopus, several transcriptomic and proteomic studies have been published, including adult specific organs [ 21 , 22 , 23 ] and paralarvae under different growth conditions [ 24 , 25 , 26 ]. However, the biological processes of this species are still not well understood due in part to this lack of genome sequencing and accurate annotation.…”

Methanol-induced optic neuropathy: a still-present problem

“…The toxic effect of methanol on the optic nerve and the retina may occur through the influence on the expression of key cytoskeleton proteins—actins and tubulins (beta-actin, beta-tubulin) conditioning the maintenance of the proper structure of cells, as well as cell signaling and regulating the process of apoptosis. In addition, changes in the expression of enzymes from the group of alcohol dehydrogenases: alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), long-chain acetyl-CoA dehydrogenase (VLCAD), family 5 member aldehyde dehydrogenase A1 (ALDH5A1), dehydrogenase 2 monophosphate-5' (IMPDH2), as well as proteins from the crystallin group and heat shock proteins (HSP) were also observed (Chen et al 2012 ; Huang et al 2011 ).…”