Differential Proteins Expression Distinguished Between Patients With Infectious and Noninfectious Uveitis

Pessuti, Carmen Luz; Medley, Quintus G.; Li, Ning; Huang, Chia‐Ling; Loureiro, Joseph; Banks, Angela; Zhang, Qin; Costa, Deise Fialho; Ribeiro, Kleber Silva; Nascimento, Heloisa; Muccioli, Cristina; Commodaro, Alessandra Gonçalves; Huang, Qian; Belfort, Rubens

doi:10.1080/09273948.2022.2150224

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…Diagnostic differentiation of infectious and noninfectious uveitis often represents a clinical challenge with important consequences for therapeutic decisions. A recent study applied an aptamer-based proteomic assay and identified thousands of AH proteins in patients with uveitis . Comparing the proteomic profiles of infectious and noninfectious uveitis revealed protein signatures that significantly differed between both etiologies.…”

Section: Aqueous Humor Liquid Biopsy Proteomicsmentioning

confidence: 99%

“…A recent study applied an aptamer-based proteomic assay and identified thousands of AH proteins in patients with uveitis. 87 Comparing the proteomic profiles of infectious and noninfectious uveitis revealed protein signatures that significantly differed between both etiologies. In contrast, the plasma proteome of the same patients did not reveal significant differences between both groups, further highlighting the value of liquid biopsies from locally enriched fluids, such as the AH compared to the blood.…”

Section: Aqueous Humor Liquid Biopsy Proteomicsmentioning

confidence: 99%

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Liquid Biopsy Proteomics in Ophthalmology

Wolf,

Franco,

Yip

et al. 2024

J. Proteome Res.

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Minimally invasive liquid biopsies from the eye capture locally enriched fluids that contain thousands of proteins from highly specialized ocular cell types, presenting a promising alternative to solid tissue biopsies. The advantages of liquid biopsies include sampling the eye without causing irreversible functional damage, potentially better reflecting tissue heterogeneity, collecting samples in an outpatient setting, monitoring therapeutic response with sequential sampling, and even allowing examination of disease mechanisms at the cell level in living humans, an approach that we refer to as TEMPO (Tracing Expression of Multiple Protein Origins). Liquid biopsy proteomics has the potential to transform molecular diagnostics and prognostics and to assess disease mechanisms and personalized therapeutic strategies in individual patients. This review addresses opportunities, challenges, and future directions of high-resolution liquid biopsy proteomics in ophthalmology, with particular emphasis on the large-scale collection of high-quality samples, cutting edge proteomics technology, and artificial intelligence-supported data analysis.

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Section: Aqueous Humor Liquid Biopsy Proteomicsmentioning

confidence: 99%

Section: Aqueous Humor Liquid Biopsy Proteomicsmentioning

confidence: 99%

Liquid Biopsy Proteomics in Ophthalmology

Wolf,

Franco,

Yip

et al. 2024

J. Proteome Res.

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“…Following biotinylation of captured proteins and cleavage of SOMAmer/protein complexes from the beads using UV light, the complexes are recaptured on fresh beads via the biotinylated proteins and SOMAmers eluted and analyzed on a microarray chip with signal intensity correlating to protein concentration. A first study using the SomaScan platform for ocular fluid analysis was conducted by Pessuti et al (2023) . AH from 28 patients with infectious uveitis and 29 samples from non-infectious uveitis patients was analyzed in comparison to 35 AH samples from cataract patients, using the SomaScan v3 Assay, identifying a minimum of 4,074 proteins across all groups.…”

Section: Deciphering the Ah Proteomementioning

confidence: 99%

Advances in aqueous humor proteomics for biomarker discovery and disease mechanisms exploration: a spotlight on primary open angle glaucoma

Beutgen,

Graumann

2024

Front. Mol. Neurosci.

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Altered protein levels in the aqueous humor (AH) may be a valuable source of novel biomarkers in neurodegenerative retinal disease. The proximity of this body fluid to the disease focus, and its corresponding enrichment for tissue specific proteins, renders it an excellent matrix to study underlying molecular mechanisms. Novel proteomic methods accordingly hold large potential for insight into pathologies based on the composition of the AH proteome, including primary open angle glaucoma (POAG). Recent mass spectrometry-based studies use novel approaches to tackle the challenges arising from the combination of low available sample volume and protein concentration, thereby increasing proteome coverage. But despite significant improvements in mass spectrometry (MS), a different class of proteomic technologies is poised to majorly impact the analysis of ocular biofluids. Affinity proteomic workflows, having become available commercially recently, have started to complement data obtained by MS and likely will grow into a crucial tool for ophthalmological biomarker research. This review highlights corresponding approaches in proteome analysis of aqueous humor and discusses recent findings on alterations of the AH proteome in POAG.

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“…Consequently, researchers have investigated changes within the AH proteome associated with different pathophysiological conditions ( 7 , 8 , 10–26 ). These investigations have led to evidence linking specific AH proteins to ocular diseases, such as cataracts ( 12 , 15 , 19 , 23 ), glaucoma ( 15 , 18 , 21 , 27 ), age-related macular degeneration ( 20 , 22 ), uveitis ( 28 , 29 ), retinoblastoma ( 30 , 31 ) and diabetic retinopathy ( 17 , 24 ). Moreover, the dynamics of the AH proteome have been observed to vary across demographic variables, such as sex ( 13 ) and race ( 32 ), with certain proteins involved in inflammatory protection and immune response found to be more abundant in AH samples from female and African American cohorts, respectively.…”

Section: Introductionmentioning

confidence: 99%

AHP DB: a reference database of proteins in the human aqueous humor

Lee,

Goyal,

Jones

et al. 2024

Database

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The aqueous humor (AH) is a low-viscosity biofluid that continuously circulates from the posterior chamber to the anterior chamber of the eye. Recent advances in high-resolution mass-spectrometry workflows have facilitated the study of proteomic content in small-volume biofluids like AH, highlighting the potential clinical implications of the AH proteome. Nevertheless, in-depth investigations into the role of AH proteins in ocular diseases have encountered challenges due to limited accessibility to these workflows, difficulties in large-scale AH sample collection and the absence of a reference AH proteomic database. In response to these obstacles, and to promote further research on the involvement of AH proteins in ocular physiology and pathology, we have developed the web-based Aqueous Humor Proteomics Database (AHP DB). The current version of AHP DB contains proteomic data from 307 human AH samples, which were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The database offers comprehensive information on 1683 proteins identified in the AH samples. Furthermore, relevant clinical data are provided for each analyzed sample. Researchers also have the option to download these datasets individually for offline use, rendering it a valuable resource for the scientific community. Database URL: https://ahp.augusta.edu/

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Differential Proteins Expression Distinguished Between Patients With Infectious and Noninfectious Uveitis

Cited by 4 publications

References 39 publications

Liquid Biopsy Proteomics in Ophthalmology

Liquid Biopsy Proteomics in Ophthalmology

Advances in aqueous humor proteomics for biomarker discovery and disease mechanisms exploration: a spotlight on primary open angle glaucoma

AHP DB: a reference database of proteins in the human aqueous humor

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