Differential protein expression of Caco-2 cells treated with selenium nanoparticles compared with sodium selenite and selenomethionine

Abstract

The study was designed to determine the differential protein expression of Caco-2 cells treated with different forms of selenium including sodium selenite, selenomethionine (Se-Met), and selenium nanoparticles (nano-Se). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS) were used to identify the differentially expressed proteins. The results indicated that seven protein spots, ubiquitin-conjugating enzyme E2 (E2), glutathione synthetases (GS), triosephosphate isomerase (TSP), T-complex protein 1 subunit zeta (TCPZ), lamin-B1, heterogeneous nuclear ribonucleoprotein F (hnRNP F), and superoxide dismutase [Cu-Zn] (Cu, Zn-SOD) were significantly different among all the groups. According to the order of control, sodium selenite, Se-Met, and Nano-Se, the expression levels of two proteins (E2 and GS) increased and the other differential proteins were reverse. Except for E2, there were no significant differences in other protein expressions between the groups treated with nano-Se and Se-Met.

Lithium as a prooxidant? A possible protective role of selenium – in vitro study

“…In contrast, both Se doses caused well-marked decrease in total extracellular GSH [5]. The comparison of three Se-form (sodium selenite, selenomethionine and nanoparticles 100 nM of Se) revealed an increase in expression of glutathione synthase in Caco-2 cells, but two latter forms exerted a significantly greater effect [9]. In A549 human lung cancer cell line sodium selenite (0.5 mM) did not alter GSH content, while organic selenium (ethaselen 3 mM) and combination of these two forms caused GSH decrease, being intensified along the time of experiment [25].…”

“…Selenium generally caused a slight increase in SOD activity, but methylseleninic acid was the most effective form [21]. Fu et al also compared three Seform (sodium selenite, selenomethionine and nanoparticles at a dose of 100 nM of Se) and showed that expression of superoxide dismutase (Cu-Zn isoform) in Caco-2 cells was decreased, particularly by the two latter forms [9].…”

“…Inorganic selenium (sodium selenite) has been found to prevent oxidative stress caused by methamphetamine in neuronal cells [5] and by As 2 O 3 in fish hepatoma cells [6], as well as t-butyl hydroperoxide-induced DNA damage in human mesenchymal stromal cells [7]. Other forms (organic compounds and nanoparticles) of selenium have also been studied and the research has revealed the dependence of the effects on the used form [3,[8][9][10].…”

Combination of Sodium Selenite and Doxorubicin Prodrug Ac-Phe-Lys-PABC-ADM Affects Gastric Cancer Cell Apoptosis in Xenografted Mice

“…Under normal physiological conditions, high selenium content in the serum is positively related to survival rate, while a decrease in serum selenium levels increases the risk of cancer and death [6]. In particular, sodium selenite (SS) is a common inorganic selenium that triggers superoxide anions to induce apoptosis in cancer cells [7, 8], demonstrating its potential anticancer activity.…”

Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

“…Using 2DE‐based proteomics the influence of Se compounds on cancer cells, e.g. prostate cancer lines , HeLa cells and Caco‐2 cells , on rat plasma and on different rodent tissues has been previously investigated . However, in all these studies only effects of single selenocompounds have been evaluated.…”