Differential protein expression induced by a lipid extract of Perna canaliculus in splenocytes of rats with adjuvant-induced arthritis

Abstract

The lipid extract of Perna canaliculus (Lyprinol) has known anti-inflammatory effects. However, the only information on mechanisms is regulation of cytokine secretion. Therefore, we conducted a proteomic study exploring the effects of Lyprinol on protein expression in splenocytes collected from AIA rats. Splenocytes from AIA rats fed with Lyprinol had increased protein expression of malate dehydrogenase (MDH). Lyprinol also decreased the expressions of 5 other proteins: protein-o-mannosyl- transferase 2 (PMT-2), Tdrd 7, telethonin, dynactin 2 and protein disulfide isomerase (PDI or glucose-regulated protein (GRP)). Besides MDH, PMT- 2, titin-cap protein and protein disulfide isomerase (PDI) are known to be related to metabolism. However, it is currently unknown if Lyprinol administration decreases metabolic glucose in the body and alleviates symptoms of inflammation and arthritis. Further experiments are required to correlate levels of citric acid intermediates and glucose to the severity of inflammation and pain in AIA rats fed Lyprinol.

Furan fatty acid as an anti-inflammatory component from the green-lipped mussel Perna canaliculus

“…Finally, the mixture was separated by silica gel flash chromatography again, to afford the fractions containing the F-acid methyl esters. A GC-MS analysis revealed that these fractions predominantly contained F 4 and F 6 (Fig. 1A and Fig.…”

“…The development of a natural remedy for the management of arthritis that will not only replace nonsteroidal anti-inflammatories (NSAIDs) as the standard treatment for all types of arthritis but also treat the condition successfully, without any known side effects, is being eagerly pursued (1). Therefore, numerous studies have been published on the lipid composition of the extract (2-4), its complex mode of action (5)(6)(7)(8), activity in animal models (9,10), and efficacy in controlling osteoarthritis (11,12) and moderate asthma in patients (13). However, the active component from the mussel is a lipid rich in polyunsaturated fatty acids (PUFAs) and is therefore prone to activation of degrading enzymes including phospholipases and lipoxygenases (14,15) after cell damage, unless it is stored and transported properly.…”

Gui-ling-gao inhibits Concanavalin A-induced inflammation by suppressing the expressions of iNOS and proinflammatory cytokines in mice isolated splenocytes

“…Splenocytes were prepared from BALB/c mice by the method reported previously (Kamer et al, ; Lee, Butt, Wong, & Lo, ) but with some modifications. Briefly, splenocytes were collected from the spleen of the animals and the tissues were ground using a glass rod in acid citrate‐dextrose solution (Sigma‐Aldrich, St. Louis, MO, USA).…”

Lyprinol—Is It a Useful Anti‐Inflammatory Agent?

“…Thus, Lyprinol changed the expression of several proteins related to metabolism (increased expression of malate dehydrogenase, and decreased expression of protein- o -mannosyl-transferase 2,titin-cap protein, and protein disulfide isomerase) and decreased expression of Tdrd7, telethonin and dynactin [18]. The authors hypothesize that these changes in proteins related to metabolism may be responsible for the anti-inflammatory effects of Lyprinol.…”