Differential Protein Content between Fresh and Freeze-Dried Plasma Rich in Growth Factors Eye Drops

Abstract

The purpose of this study was to analyze the proteomic composition of plasma rich in growth factors eye drops (PRGF) in comparison to lyophilized PRGF eye drops (PRGF lyo). The differential protein expression of keratocyte (HK) cells after PRGF or PRGF lyo treatment was also determined. Blood from different donors was collected and processed to obtain PRGF and PRGF lyo eye drops. Then, HK cells were treated with both formulations. A proteomic analysis was performed to evaluate the differential proteomic profile between PRGF and PRGF lyo, and the differential protein expression by HK cells after treatment with both blood-derived products. About 280 proteins were detected between both blood-derived formulation, with only 8 of them reaching significant differences. Furthermore, 101 out of 3213 proteins showed statistically significant deregulation in HK cells after treatment with PRGF or PRGF lyo. Gene Ontology analysis showed that these significant deregulated proteins were involved in 30 functional processes. However, the Ingenuity Pathway Analysis showed that no significant differences were found in any of the identified processes. In summary, the present study show that no significant differences were found in the proteomic profile or in the signaling pathways activation in HK cells between PRGF and PRGF lyo.

Biochemical and biomechanical characterization of an autologous protein-based fibrin sealant for regenerative medicine

“…Endoret®PRGF® has been widely applied in different medical fields including traumatology, ophthalmology, maxillofacial surgery and dermatology [ 16 – 19 ], and the main preparation protocol has not suffered from major changes over the years [ 20 ]. Moreover, the versatility of this technology allows the easy and fast in situ obtention of custom-tailored pharmaceutical formulations for different biomedical applications such as eye drops, a 3D-gel, a transparent membrane, or a topical ointment [ 21 – 24 ].…”