Differential protein analysis of lymphocytes between children with acute lymphoblastic leukemia and healthy children

Abstract

We identified differential proteins in lymphocytes between patients with childhood acute lymphoblastic leukemia (c-ALL) and healthy children. Samples of bone marrow lymphocytes from children with c-ALL and peripheral blood lymphocytes from healthy children were collected, and total proteins were extracted and separated from these samples followed by two-dimensional gel electrophoresis for comparative analysis. The differential protein spots in c-ALL cells were digested in situ, and then analyzed with matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF-MS) followed by identification using the relevant database. Fifteen differential expression proteins were obtained by comparative proteomics analysis. Of the 15 differential proteins, eight were identified. Of the eight proteins, two had high expression and six low expression in c-ALL cells. The eight differential proteins are expected to become new diagnostic markers and drug targets for c-ALL.

Proteomics in Childhood Acute Lymphoblastic Leukemia: Challenges and Opportunities

“…Moreover, differential proteins were analyzed by MALDI-TOF-MS and were identified in lymphocytes in patients with childhood ALL and healthy children, by Wang et al [74]. Among the 25 differential proteins, eight provided a valuable insight into the molecular mechanism of leukemogenesis and could serve as candidate markers or drug targets.…”

Microwave and Magnetic (M2) Proteomics of Childhood B-ALL

“…This is particularly true for studies seeking to identify novel protein biomarkers and therapeutic targets in blood, where proteins from hundreds of different cell types can be present in a relative abundance that may span up to twelve orders of magnitude. Masking problems can be partially overcome by numerous sample preparation strategies, including affinity depletion of high abundant proteins, enrichment of low abundance proteins, and fractionation of proteins by either two-dimensional electrophoresis [5][6][7] or liquid chromatography [8]. While improving selectivity, these strategies suffer from poor sample throughput and reproducibility due to lengthy and complex sample preparation times and poor dynamic range due to adsorptive losses at various steps.…”

“…In contrast, there are only a few reports of proteomic analysis of childhood ALL [6,7,[9][10][11][12][13][14]. Significantly, few previous studies have employed isobaric labeling techniques such as 'tandem mass tagging' (TMT) [15,16] or 'super' stable isotopic labeling of amino acids in culture (SILAC) [17,18] for quantitative MS/MS-based proteomics.…”

<p>Proteomic analysis of cerebrospinal fluid in pediatric acute lymphoblastic leukemia patients: a pilot study</p>

“…Proteomic technology enables people to observe the development and progression of diseases dynamically and to carry out quantitative analysis 16–18. Proteomic analysis of biological fluids has been widely used to screen and identify disease markers 19.…”

“…Proteomic analysis of biological fluids has been widely used to screen and identify disease markers 19. Recently, various groups have attempted to discover potential protein biomarkers of pediatric leukemia 18–21. One study applied surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to analyze the proteomes of cell lysates from childhood leukemia cell lines and childhood leukemia bone marrow samples of different subtypes 22.…”