Differential protective effects of connective tissue growth factor against Aβ neurotoxicity on neurons and glia

Yang, Cheng-Ning; Wu, Minfang; Liu, Chung‐Chih; Jung, Wei‐Hung; Chang, Yu‐Chin; Lee, Wang-Pao; Shiao, Young‐Ji; Wu, Chia‐Lin; Liou, Horng-Huei; Lin, Sze‐Kwan; Chan, Chih‐Chiang

doi:10.1093/hmg/ddx278

Cited by 19 publications

(14 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…qRT‐PCR was performed following the guideline of (Bustin et al., 2019). The procedures were as previously described in (Yang et al., 2017). Further details are provided in Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Lifespan regulation in α/β posterior neurons of the fly mushroom bodies by Rab27

Lien

Chen

et al. 2020

Aging Cell

View full text Add to dashboard Cite

The desire for lifespan extension is a never-ending quest throughout human history. While extreme lifespans are likely limited genetically, environmental factors and acquired characteristics do affect the speed of degeneration and health span. As a result, lifespan is determined as the collective effect of internal and external factors, including the challenges of oxidative stress, the homeostasis of circulating metabolites, intake of energy and nutrients, and the allocation of resources among major life functions. Restriction of caloric intake is by far the most effective approach to extend lifespan in all species examined from yeast to non-human primates. Although its long-term effect is hard to evaluate in humans, caloric restriction has been shown to induce physiological changes that are similar to those observed in animal models. However, when energy intake is restricted, limited resources need to be relocated from growth and reproduction to maintain life-sustaining functions, resulting in trade-offs between

show abstract

Section: Methodsmentioning

confidence: 99%

“…Conditioned odor avoidance was performed as previously described (Yang et al., 2017). Further details are provided in Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Lifespan regulation in α/β posterior neurons of the fly mushroom bodies by Rab27

Lien

Chen

et al. 2020

Aging Cell

View full text Add to dashboard Cite

show abstract

“…In draper mutant animals Aß toxicity is increased, whereas enhanced expression of glial Draper engulfment receptor reverses Aβ accumulation, as well as behavioral phenotypes (Ray et al, ). Similarly, expression of the connective tissue growth factor (CTGF) facilitates Aβ uptake and subsequent degradation within primary mammalian glial cells (Yang et al, ). Interestingly, this also operates in Drosophila, where glial‐expression of CTGF reduced Aβ deposits, improved locomotor function, and rescued memory deficits again highlighting the close evolutionary relationships between fly and mammalian glial cells (Yang et al, ).…”

Section: Conservation Of Processesmentioning

confidence: 99%

Drosophila glia: Few cell types and many conserved functions

Yildirim

Petri

Kottmeier

et al. 2018

Glia

138

153

View full text Add to dashboard Cite

Glial cells constitute without any dispute an essential element in providing an efficiently operating nervous system. Work in many labs over the last decades has demonstrated that neuronal function, from action potential generation to its propagation, from eliciting synaptic responses to the subsequent postsynaptic integration, is evolutionarily highly conserved. Likewise, the biology of glial cells appears conserved in its core elements and therefore, a deeper understanding of glial cells is expected to benefit from analyzing model organisms such as Drosophila melanogaster. Drosophila is particularly well suited for studying glial biology since in the fly nervous system only a limited number of glial cells exists, which can be individually identified based on position and a set of molecular markers. In combination with the well‐known genetic tool box an unprecedented level of analysis is feasible, that not only can help to identify novel molecules and principles governing glial cell function but also will help to better understand glial functions first identified in the mammalian nervous system. Here we review the current knowledge on Drosophila glia to spark interest in using this system to analyze complex glial traits in the future.

show abstract

“…a Based on Affymetrix microarray gene expression data, nine out of 12 genes selected for qPCR were confirmed to be expressed in the cultured cells. b Total proteins were isolated from cell-conditioned media, separated by SDS-PAGE, transferred onto a PVDF membrane, and stained with 0.5% (w/v) Ponceau S. c Among the 5 selected genes [27][28][29][30][31][32], three encoded proteins were detected by WB to be present in the cell-conditioned media. d The neuroprotective effect of Vegfa was evaluated at the indicated time points by OKR after an intravitreal injection of the anti-VEGF antibody (bevacizumab) into Rho P23H/+ and e Rho +/+ mice at P40 secretory factors (Vegfa [27][28][29], Ctgf [30], Adnp [31], Grn [32], and Efemp2), i.e., Vegfa, Grn, and Efemp2, were detected by WB (Fig.…”

Section: Detection Of Factors That Cells Secreted Into the Cell Cultumentioning

confidence: 99%

Paracrine effects of intraocularly implanted cells on degenerating retinas in mice

Liu

Chen

et al. 2020

Stem Cell Res Ther

View full text Add to dashboard Cite

Background: Retinal degeneration is a leading cause of blindness in the world; its etiology is complex and involves genetic defects and stress-associated aging. In addition to gene therapies for known genetically defective retinal degeneration, cellular therapies have been widely explored for restoring vision in both preclinical animal models and clinical trials. Stem cells of distinct tissue sources and their derived lineages have been tested for treating retinal degeneration; most of them were reported to be effective to some extent in restoring/improving deteriorated vision. Whether this visual improvement is due to a functional integration of grafted cells to substitute for lost retinal neurons in recipients or due to their neuroprotective and neurotrophic effects to retain recipient functional neurons, or both, is still under debate. Methods: We compared the results of subretinal transplantation of various somatic cell types, such as stem cells and differentiated cells, into Rho P23H/+ mice, a retinal degeneration model for human retinitis pigmentosa (RP) by evaluating their optokinetic response (OKR) and retinal histology. We identified some paracrine factors in the media that cultured cells secreted by western blotting (WB) and functionally evaluated the vascular endothelial growth factor Vegfa for its potential neurotrophic and neuroprotective effects on the neuroretina of model animals by intravitreal injection of VEGF antibody. Results: We found that live cells, regardless of whether they were stem cells or differentiated cell types, had a positive effect on improving degenerating retinas after subretinal transplantation; the efficacy depended on their survival duration in the host tissue. A few paracrine factors were identified in cell culture media; Vegfa was the most relevant neurotrophic and neuroprotective factor identified by our experiments to extend neuron survival duration in vivo. Conclusions: Cellular therapy-produced benefits for remediating retinal degeneration are mostly, if not completely, due to a paracrine effect of implanted cells on the remaining host retinal neurons.

show abstract

Differential protective effects of connective tissue growth factor against Aβ neurotoxicity on neurons and glia

Cited by 19 publications

References 56 publications

Lifespan regulation in α/β posterior neurons of the fly mushroom bodies by Rab27

Lifespan regulation in α/β posterior neurons of the fly mushroom bodies by Rab27

Drosophila glia: Few cell types and many conserved functions

Paracrine effects of intraocularly implanted cells on degenerating retinas in mice

Contact Info

Product

Resources

About