Differential Pigment Accumulation in Carrot Leaves and Roots during Two Growing Periods

Abstract

Carotenoids are important secondary metabolites involved in plant growth and nutritional quality of vegetable crops. These pigments are highly accumulated in carrot root, but knowledge about the impact of environmental factors on their accumulation is limited. The purpose of this work was to investigate the impact of environmental variations on carotenoid accumulation in carrot leaves and roots. In this work, carrots were grown during two contrasting periods to maximize bioclimatic differences. In leaves, carotenoid and chlorophyll contents were lower in the less favorable growing conditions, whereas relative contents were well conserved for all genotypes, suggesting a common regulatory mechanism. The down-regulation of all genes under environmental constraints demonstrates that carotenoid accumulation is regulated at the transcriptional level. In roots, the decrease in α-carotene and lutein contents was accompanied by an increase of β-carotene relative content. At the transcriptional level, LCYB and ZEP expression increased, whereas LCYE expression decreased, in the less favorable conditions, suggesting that carotenoid biosynthesis is switched toward the β-branch.

Carrot Genotypes Contrasted by Root Color and Grown under Different Conditions Displayed Differential Pharmacological Profiles in Vascular and Metabolic Cells

“…The carotenoid composition was analyzed in the ethanol extracts only, as DMSO is a solvent methodologically incompatible with system used in our experimental condition. Carotenoid content in 100µL of ethanol extracts was measured according to Perrin et al [19] by HPLC system (Shimadzu Corporation, Kyoto, Japan) equiped with a thermostated autosampler (SIL-10AD VP), a diode array detector (SPD-M10A VP) and aYMC C30 (YMC, Japan) column (150 × 4.6; 3 µm). The elution program was as followed for solvents A and B: 0-12 min, 80-45%; 12-14 min, 45-10%; 14-17 min, 10-80%; and 17-23 min, 80%.…”

ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes

“…Chlorophyll concentrations were measured according to Moran (1982) on a FluoStar Omega spectrophotometer (BMG Labtech). For HPLC analysis, pigment extraction and quantification were performed as described by Perrin et al (2016). Briefly, the carotenoid measurements were performed with a Shimadzu HPLC equipped with a thermostated autosampler (SIL-10ADVP) and a diode array detector (SPD-M10AVP).…”

Pro‐vitamin A carotenoids in East African highland banana and other Musa cultivars grown in Uganda

“…Changes in temperature, light intensity, and rainfall have been implicated in influencing carotenoid accumulation in crops such as pumpkin (Jaswir, Shahidan, Othman, Has-Yun Hashim, Octavianti, & Salleh 2014) carrot (Perrin et al, 2016) maize (Ortiz, Rocheford, & Ferruzzi, 2016) and banana (Davey et al, 2007;Paul et al, 2017). Changes in temperature, light intensity, and rainfall have been implicated in influencing carotenoid accumulation in crops such as pumpkin (Jaswir, Shahidan, Othman, Has-Yun Hashim, Octavianti, & Salleh 2014) carrot (Perrin et al, 2016) maize (Ortiz, Rocheford, & Ferruzzi, 2016) and banana (Davey et al, 2007;Paul et al, 2017).…”