Differential Phosphorylation of MAPK Isoforms in Keratinocyte Cell Line by Contact Allergens and Irritant

Abstract

Mitogen-activated protein kinase (MAPK) is an important mediator of cellular signaling pathways. In an attempt to investigate the role of these kinases in contact allergen/irritant-induced signaling cascades, we have utilized keratinocyte (A431 cell line) as antigen presenting cells (APCs). This group has previously reported the applicability of A431 as an APC. The role of these kinases in the elicitation pathways of allergic contact dermatitis was investigated using interleukin-12p40 (IL-12p40) as a marker. Inhibitors of the isoforms of MAPK reduced the allergen-induced IL-12 release in the A431 cell line. This result suggests a possible role of the isoforms of MAPK in the development of contact allergy.

Keratinocytes, Innate Immunity and Allergic Contact Dermatitis - Opportunities for the Development of In Vitro Assays to Predict the Sensitizing Potential of Chemicals

“…p38 MAPK are enzymes that play an important role in the signal transduction of TLR (Mehrotra et al, 2007). In mice treated with the specific p38 MAPK inhibitor SB202190 the ear swelling in response to DNFB was impaired (Takanami-Ohnishi et al, 2002).…”