Differential Oxidative Stress Induced by Dengue Virus in Monocytes from Human Neonates, Adult and Elderly Individuals

Valero, Nereida; Mosquera, Jesús; Áñez, Germán; Levy, Alegría; Marcucci, Rafael; Álvarez-Mon, Melchor

doi:10.1371/journal.pone.0073221

Cited by 27 publications

(27 citation statements)

References 37 publications

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“…The similar effects of salubrinal and NAC on ROS levels suggest a PERK-dependent ROS production during dengue infection, much like another Flaviviridae (hepatitis C virus (HCV)); however, this does not necessarily kill infected cells. 64 , 65 Neither KU55933 (inhibitor of ATM) nor wortmannin (inhibitor of autophagy) had any effect on the ROS levels. Therefore, ER stress signaling, but not ATM activity, regulates the increase in ROS during infection.…”

Section: Discussionmentioning

confidence: 98%

Dengue-induced autophagy, virus replication and protection from cell death require ER stress (PERK) pathway activation

et al. 2016

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A virus that reproduces in a host without killing cells can easily establish a successful infection. Previously, we showed that dengue-2, a virus that threatens 40% of the world, induces autophagy, enabling dengue to reproduce in cells without triggering cell death. Autophagy further protects the virus-laden cells from further insults. In this study, we evaluate how it does so; we show that dengue upregulates host pathways that increase autophagy, namely endoplasmic reticulum (ER) stress and ataxia telangiectasia mutated (ATM) signaling followed by production of reactive oxygen species (ROS). Inhibition of ER stress or ATM signaling abrogates the dengue-conferred protection against other cell stressors. Direct inhibition of ER stress response in infected cells decreases autophagosome turnover, reduces ROS production and limits reproduction of dengue virus. Blocking ATM activation, which is an early response to infection, decreases transcription of ER stress response proteins, but ATM has limited impact on production of ROS and virus titers. Production of ROS determines only late-onset autophagy in infected cells and is not necessary for dengue-induced protection from stressors. Collectively, these results demonstrate that among the multiple autophagy-inducing pathways during infection, ER stress signaling is more important to viral replication and protection of cells than either ATM or ROS-mediated signaling. To limit virus production and survival of dengue-infected cells, one must address the earliest phase of autophagy, induced by ER stress.

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Section: Discussionmentioning

confidence: 98%

Dengue-induced autophagy, virus replication and protection from cell death require ER stress (PERK) pathway activation

et al. 2016

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“…Some authors approved that association between DENV‐2 and disease severity in Southeast Asia . The underlying mechanism is not fully clear, but it is reported that DENV‐2 is associated with increased nitric oxide (NO) levels and activity causing vascular leakage . That is not only for serotypes; even genotypes of the same serotype can affect.…”

Section: Discussionmentioning

confidence: 99%

“…36,38 The underlying mechanism is not fully clear, but it is reported that DENV-2 is associated with increased nitric oxide (NO) levels and activity causing vascular leakage. 39 That is not only for serotypes; even genotypes of the same serotype can affect. African and American genotypes of serotype 2 and 3 were found to be less virulent than Asian genotypes of the same serotypes.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and burden of dengue infection in Europe: A systematic review and meta‐analysis

Ahmed

Mohammed

et al. 2019

Reviews in Medical Virology

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Summary Imported dengue cases are thought to be important source for transmission of autochthonous dengue in Europe. We aimed to investigate the prevalence of dengue in Europe, its severity, and factors associated with it. Out of 5287 reports resulting from the search of nine electronic search engines, we included 174 reports. Meta‐analysis was performed by pooling the event rate and 95% confidence interval (CI). Subgroup meta‐analyses were performed to test the effect of the covariates. Among 20 284 reported cases, 130 autochthonous dengue cases were reported in eight countries with the highest number of cases reported in Israel (n = 41). The highest number of imported dengue cases was in Germany (n = 6638) then France (n = 6610). Most cases were imported from Southeast Asia (n = 2533) especially Thailand. Dengue infection cases increased with time, with 4157 cases reported in 2010. Second dengue infection and dengue serotype 2 were positively associated with dengue severity. The proportion of autochthonous dengue infection increased with time to reach 14.8% (95% CI, 7.6‐26.9) in 2015. The pooled proportion of severe dengue was 6.18% (95% CI, 2.7‐13.3). The United Kingdom and France had the highest rate of severe dengue (25%; 95% CI, 6.3‐62.3, and 21.4%; 95% CI, 24.5‐18.7, respectively). This change may be due to the surveillance efforts instead of true biological phenomenon; thus, the lack of surveillance is an obvious limitation. In conclusion, imported and autochthonous dengue has been increasing in Europe. Severe dengue began to increase recently in Europe. European health authorities should pay more attention for the diagnosis and control of dengue infection among returning travelers, especially the travelers with fever of unknown origin.

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“…Valero et al showed that monocytes from neonates, adults, and elderly patients infected with different strains of dengue viruses responded differently. For most strains, as well as LPS, monocytes from adults showed the most prominent response by secreting more NO than others [61]. In contrast, some studies suggest a greater production of NO by neonates [31,62].…”

Section: Nitric Oxide As a Prognostic Markermentioning

confidence: 92%

Role of nitric oxide in immune responses against viruses: beyond microbicidal activity

2015

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Differential Oxidative Stress Induced by Dengue Virus in Monocytes from Human Neonates, Adult and Elderly Individuals

Cited by 27 publications

References 37 publications

Dengue-induced autophagy, virus replication and protection from cell death require ER stress (PERK) pathway activation

Dengue-induced autophagy, virus replication and protection from cell death require ER stress (PERK) pathway activation

Prevalence and burden of dengue infection in Europe: A systematic review and meta‐analysis

Role of nitric oxide in immune responses against viruses: beyond microbicidal activity

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