Differential oxidative damage and expression of stress defence regulons in culturable and non‐culturable <i>Escherichia coli</i> cells

Desnues, Benoît; Cuny, C.; Grégori, Gérald; Dukan, Sam; Aguilaniu, Hugo; Nyström, Thomas

doi:10.1038/sj.embor.embor799

Cited by 152 publications

(149 citation statements)

References 31 publications

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“…Oxidatively damaged proteins increase during aerobic carbon starvation (Nystrom, 2004;Dukan and Nystrom, 1999), and cells which fail to control the burden of oxidized proteins lose viability (Desnues et al, 2003). We thus speculate that the aerobically-driven increase in oxidized proteins may be mechanistically important for induction of the adaptive response in Lon deficient cells.…”

Section: How Are Lon Deficient Cells Protected During Aerobic Carbon mentioning

confidence: 87%

Lon protease promotes survival of Escherichia coli during anaerobic glucose starvation

Luo¹,

McNeill²,

Myers³

et al. 2007

Arch Microbiol

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In Escherichia coli, Lon is an ATP-dependent protease which degrades misfolded proteins and certain rapidly-degraded regulatory proteins. Given that oxidatively damaged proteins are generally degraded rather than repaired, we anticipated that Lon deficient cells would exhibit decreased viability during aerobic, but not anaerobic, carbon starvation. We found that the opposite actually occurs. Wild-type and Lon deficient cells survived equally well under aerobic conditions, but Lon deficient cells died more rapidly than the wild-type under anaerobiosis. Aerobic induction of the Clp family of ATP-dependent proteases could explain these results, but direct quantitation of Clp protein established that its level was not affected by Lon deficiency and overexpression of Clp did not rescue the cells under anaerobic conditions. We conclude that the Lon protease supports survival during anaerobic carbon starvation by a mechanism which does not depend on Clp.

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Section: How Are Lon Deficient Cells Protected During Aerobic Carbon mentioning

confidence: 87%

Lon protease promotes survival of Escherichia coli during anaerobic glucose starvation

Luo¹,

McNeill²,

Myers³

et al. 2007

Arch Microbiol

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“…In addition, the data imply that cytokinesis during binary fission is also inherently asymmetrical. Indeed, previous to these studies it has been shown that cells of an E. coli population consist of two discrete populations with respect to damage: a damage-enriched and a damage-free population [33]. The damage-free cells remain reproductively competent in stationary phase, whereas the damage-enriched cells eventually become genetically dead (non-culturable).…”

Section: Spatial Quality Control and Ageing In Bacteriamentioning

confidence: 99%

Spatial protein quality control and the evolution of lineage-specific ageing

Nyström

2011

Phil. Trans. R. Soc. B

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Propagation of a species requires periodic cell renewal to avoid clonal extinction. Sexual reproduction and the separation of germ cells from the soma provide a mechanism for such renewal, but are accompanied by an apparently mandatory ageing of the soma. Data obtained during the last decade suggest that a division of labour exists also between cells of vegetatively reproducing unicellular organisms, leading to the establishment of a soma-like and germ-like lineage with distinct fitness and longevity characteristics. This division of labour in both bacteria and yeast entails segregation of damaged and aggregated proteins such that the germ-like lineage is kept free of damage to the detriment of the soma-like lineage. In yeast, this spatial protein quality control (SQC) encompasses a CCT-chaperonin-dependent translocation and merging of cytotoxic protein aggregates. This process is regulated by Sir2, a protein deacetylase that modulates the rate of ageing in organisms ranging from yeast to worms and flies. Recent data also demonstrate that SQC is intimately integrated with the machinery establishing proper cell polarity and that this machinery is required for generating a soma-like and germ-like lineage in yeast. Deciphering the details of the SQC network may increase our understanding of the development of age-related protein folding disorders and shed light on the selective forces that paved the way for polarity and lineage-specific ageing to evolve.

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“…To deal with oxidative stress, bacteria are known to evolve protective responses including the peroxidase-, catalase-, and superoxide dismutase (SOD)-mediated detoxification of ROS intermediates and repair of oxidative damage to bacterial DNA (Rosner and Storz, 1997;Desnues et al, 2003;Noor et al, 2009aNoor et al, , 2009bMurata et al, 2012;Munna et al, 2013;Nagamitsu et al, 2013). In addition, Escherichia coli produces cytoplasmic Mn-SOD (sodA) and Fe-SOD (sodB), which protect DNA and proteins from oxidation, while periplasmic Cu/Zn-SOD (sodC) protects the periplasmic and membrane constituents from exogenous O 2 - (Cabiscol et al, 2000;Moat et al, 2002;Rensing and Grass, 2003;Wang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Study of exogenous oxidative stress response in Escherichia coli, Pseudomonas spp., Bacillus spp., and Salmonella spp.

Nur¹,

Munna²,

Noor³

2014

Turk J Biol

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With a previous observation of Escherichia coli growth cessation with the supplementation of 3 mM hydrogen peroxide (H 2 O 2 ) at the late log phase, the current study further demonstrated the consequences of the addition of an increased concentration (6 mM) of H 2 O 2 and further extended the investigation on such an oxidant's impact on the growth of Salmonella spp., Pseudomonas spp., and Bacillus spp. Cell culturability was measured through the enumeration of colony-forming units (CFUs) on agar plates for up to 72 h. Subsequent changes in cell morphology and arrangements were monitored, and the cell viability was simultaneously retraced by spot tests. A sharp decline in the culturable cells of E. coli was observed after 48 h with a large mass of cell aggregates upon addition of H 2 O 2 , while Pseudomonas spp. lost viability after 36 h. Impaired morphology of such stressed cells was comparable to those of the untreated cells. Notably, Pseudomonas cells were more prone to oxidative damage compared to E. coli. In contrast, the impact of H 2 O 2 was insignificant in the case of Salmonella spp. and Bacillus spp., suggestive of a stringent defense mechanism against oxidative stress.

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Differential oxidative damage and expression of stress defence regulons in culturable and non‐culturable Escherichia coli cells

Cited by 152 publications

References 31 publications

Lon protease promotes survival of Escherichia coli during anaerobic glucose starvation

Lon protease promotes survival of Escherichia coli during anaerobic glucose starvation

Spatial protein quality control and the evolution of lineage-specific ageing

Study of exogenous oxidative stress response in Escherichia coli, Pseudomonas spp., Bacillus spp., and Salmonella spp.

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