Differential Overlap in Human and Animal Fecal Microbiomes and Resistomes in Rural versus Urban Bangladesh

Swarthout, Jenna; Fuhrmeister, Erica R.; Hamzah, Latifah; Harris, Angela; Ahmed, Minhajuddin; Gurley, Emily S.; Satter, Syed Moinuddin; Boehm, Alexandria B.; Pickering, Amy J.

doi:10.1128/aem.00759-22

Cited by 3 publications

(7 citation statements)

References 94 publications

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“…The overall predominance of tetracycline resistance genes in the present study parallels a previous study showing these genes were prevalent in humans, goats, and chicken feces in Bangladesh ( 48 ). Tetracycline resistance genes have also been shown to dominate the resistomes of human, pig, and poultry feces in Chinese wet markets ( 49 ).…”

Section: Discussionsupporting

confidence: 90%

“…A recent publication by Swarthout et al ( 48 ) used long-read sequencing to compare fecal resistomes of humans, goats, and chickens in urban and rural households in Bangladesh. There are several experimental and methodological differences between the aforementioned study and our current work.…”

Section: Discussionmentioning

confidence: 99%

“…Firstly, only household backyard poultry (rural and urban) were sampled, whereas the current study sampled commercially reared broilers sold in urban wet markets and backyard poultry in rural households. Secondly, Swarthout et al ( 48 ) separated urban and rural samples and then pooled DNA extractions, limiting the ability to discern the level of variability between individuals within these two groups. Thirdly, the current work obtained samples from poultry ceca whereas Swarthout et al ( 48 ) sampled poultry feces collected from the environment.…”

Section: Discussionmentioning

confidence: 99%

“…Secondly, Swarthout et al ( 48 ) separated urban and rural samples and then pooled DNA extractions, limiting the ability to discern the level of variability between individuals within these two groups. Thirdly, the current work obtained samples from poultry ceca whereas Swarthout et al ( 48 ) sampled poultry feces collected from the environment.…”

Section: Discussionmentioning

confidence: 99%

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Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh

Williams,

Rousham,

Neal

et al. 2023

Microbiol Spectr

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Interactions between humans, animals, and the environment are considered critical foci for addressing antimicrobial resistance (AMR). However, One Health data on AMR in low- and middle-income countries are presently scarce. Using metagenomics, we investigated whether and how the fecal antibiotic resistomes of humans are influenced by exposure to intensive and non-intensively reared poultry within contrasting settings of urban wet markets ( n = 13) and rural households ( n = 7) in Bangladesh. We also considered poultry ( n = 10) and wastewater ( n = 10) resistomes in these settings. We found that occupational poultry exposures did not significantly alter the human fecal resistome. In contrast, macrolide-lincosamide-streptogramin and streptothricin antibiotic resistance genes (ARGs) were enriched in poultry from urban wet markets relative to rural household chickens. Wastewater had the highest ARG richness, though this was only partially explained by poultry cecal and human fecal sources. Wastewater also contained clinically significant carbapenem ARGs. This study therefore provides critical insight into the distribution of ARGs in Bangladesh. IMPORTANCE Through the use of DNA sequencing, our study shows that there is no significant difference in the antibiotic resistance genes found in stool samples taken from individuals with high exposure to poultry routinely fed antibiotics and those without such exposure. This finding is significant as it suggests limited transmission of antibiotic resistance genes between poultry and humans in these circumstances. However, our research also demonstrates that commercially reared poultry are more likely to possess resistance genes to antibiotics commonly administered on medium-sized farms. Additionally, our study highlights the under-explored potential of wastewater as a source of various antibiotic resistance genes, some of which are clinically relevant.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh

Williams,

Rousham,

Neal

et al. 2023

Microbiol Spectr

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“…The relations between the resistomes of different environments can be established with the overlap of the bacteriome and resistomes recovered from the different components within the One Health framework [ 160 ]. Considerable resistome overlap has been detected within the gut of humans and animals as evidence of the interchange of antibiotic resistant genes between these entities [ 161 ]. A resistome diversity study carried out by Lawther et al .…”

Section: Antibiotic Resistance Genes and One Healthmentioning

confidence: 99%

Review of antibiotic-resistant bacteria and antibiotic resistance genes within the one health framework

Ajayi,

Odeyemi,

Akinjogunla

et al. 2024

Infection Ecology & Epidemiology

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Background: The interdisciplinary One Health (OH) approach recognizes that human, animal, and environmental health are all interconnected. Its ultimate goal is to promote optimal health for all through the exploration of these relationships. Antibiotic resistance (AR) is a public health challenge that has been primarily addressed within the context of human health and clinical settings. However, it has become increasingly evident that antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) that confer resistance are transmitted and circulated within humans, animals, and the environment. Therefore, to effectively address this issue, antibiotic resistance must also be considered an environmental and livestock/wildlife problem. Objective: This review was carried out to provide a broad overview of the existence of ARB and ARGs in One Health settings. Methods: Relevant studies that placed emphasis on ARB and ARGs were reviewed and key findings were accessed that illustrate the importance of One Health as a measure to tackle growing public and environmental threats. Results: In this review, we delve into the complex interplay of the three components of OH in relation to ARB and ARGs. Antibiotics used in animal husbandry and plants to promote growth, treat, and prevent infectious diseases lead to the development of antibiotic-resistant bacteria in animals. These bacteria are transmitted from animals to humans through food and environmental exposure. The environment plays a critical role in the circulation and persistence of antibiotic-resistant bacteria and genes, posing a significant threat to human and animal health. This article also highlights how ARGs are spread in the environment through the transfer of genetic material between bacteria. This transfer can occur naturally or through human activities such as the use of antibiotics in agriculture and waste management practices. Conclusion: It is important to integrate the One Health approach into the public health system to effectively tackle the emergence and spread of ARB and genes that code for resistance to different antibiotics.

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Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

Zhao,

Liu,

Tan

et al. 2024

Environment International

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Differential Overlap in Human and Animal Fecal Microbiomes and Resistomes in Rural versus Urban Bangladesh

Cited by 3 publications

References 94 publications

Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh

Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh

Review of antibiotic-resistant bacteria and antibiotic resistance genes within the one health framework

Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

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