Differential organ‐specific response to salt stress and water deficit in nodulated bean (<i>Phaseolus vulgaris</i>)

Verdoy, Dolores; Lucas, M. Mercedes; Manrique, Esteban; Covarrubias, Alejandra A.; Felipe, Ma Rosario de; Pueyo, José Javier

doi:10.1111/j.1365-3040.2004.01179.x

Cited by 41 publications

(25 citation statements)

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“…Diversas podem ser as respostas de tolerância dos organismos aos estresses como a resposta diferencial de cultivares MACHADO NETO et al, 2004;TEIXEIRA et al, 2008), via ativação (NEPOMUCENO et al, 2001VERDOY et al, 2004) ou desativação (NEPOMUCENO et al, 2000) de genes que codificarão proteínas específicas, finalizando com diversas respostas fisiológicas, metabólicas e de desenvolvimento. Entre estas respostas ao estresse, encontramos fatores de transcrição (ZHU, 2001), proteínas de diversas classes, como as BiP (Binding Protein) (KALINSKI et al, 1995), Peroxidases (SIEGEL, 1993), proteínas LEA (Late embryogenesis abundant protein) (VERDOY et al, 2004), proteínas ricas em prolina (PRPs) (VERDOY et al, 2004) e Proteínas de Choque Térmico (Heat Shock Protein -HSPs), as quais deveriam ser específicas ao estresse de calor, porém, também são sintetizadas na presença de estresse hídrico ou como resposta universal ao estresse (VIERLING, 1991), sendo encontradas desde em procariotos até em eucariotos.…”

Section: Introductionunclassified

“…Entre estas respostas ao estresse, encontramos fatores de transcrição (ZHU, 2001), proteínas de diversas classes, como as BiP (Binding Protein) (KALINSKI et al, 1995), Peroxidases (SIEGEL, 1993), proteínas LEA (Late embryogenesis abundant protein) (VERDOY et al, 2004), proteínas ricas em prolina (PRPs) (VERDOY et al, 2004) e Proteínas de Choque Térmico (Heat Shock Protein -HSPs), as quais deveriam ser específicas ao estresse de calor, porém, também são sintetizadas na presença de estresse hídrico ou como resposta universal ao estresse (VIERLING, 1991), sendo encontradas desde em procariotos até em eucariotos.…”

Section: Introductionunclassified

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Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Coelho¹,

Agostini²,

Guaberto³

et al. 2010

Acta Sci. Agron.

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RESUMO.O feijão (Phaseolus vulgaris L.) é de grande expressão alimentícia. A emergência da cultura é dependente de água e considerada a fase mais crítica. Os objetivos deste trabalho foram simular a deficiência de água no início da germinação em laboratório, em sementes de feijão 'Pérola', utilizando-se: manitol, CaCl 2 , MgCl 2 e NaCl em potenciais de 0; -0,3; -0,6; -0,9 e -1,2 MPa, estabelecidos pela equação de Van't Hoff e avaliar o perfil eletroforético de proteínas totais solúveis por meio de SDS-PAGE. Foram avaliados: germinação, classificação de vigor, massa seca de raiz e de parte aérea e resposta diferencial de expressão de proteínas. O delineamento experimental foi inteiramente casualizado. Os dados foram analisados pela aplicação do teste F, para análise de variância, regressão polinomial para os níveis de potencial osmóticos para cada uma das variáveis fisiológicas estudadas. O bandeamento eletroforético foi avaliado visualmente por imagem dos géis. A simulação do estresse permitiu avaliar a drasticidade do NaCl em todos os parâmetros avaliados e a ausência de proteínas de baixo peso molecular neste osmótico. As proteínas de 110 e 30 kDa foram indicativas de estresse hídrico, mas não do salino.Palavras-chave: Phaseolus vulgaris L., deficiência de água, HSPs.ABSTRACT. Differential protein expression during germination as a result of a water deficit associated with variable osmotic pressure in snap-beans. Snap-beans (Phaseolus vulgaris L.) have a high nutritionally value. Successful seedling emergence is dependent on the availability of water and is considered the most critical phase in plant development. The objectives of this work were to simulate water deficiency at the beginning of the germination period. Snap-bean seeds of the variety 'Pérola' were submitted to osmotic stress under laboratory conditions. Mannitol, CaCl 2 , MgCl 2 and NaCl were used to develop five degrees of osmotic potential: 0, -0.3, -0.6, -0.9 and -1.2 MPa. Van't Hoff's equation was used to calculate the osmotic potential. The protein pattern of the total soluble proteins after electrophoresis was evaluated with SDS-PAGE. The response parameters were evaluated including the germination, the vigour classification, the root dry weight, the shoot dry weight and the resultant differential protein expression. The experimental design was completely randomised. Data were analysed using the F test (ANOVA), and polynomial regression was used for the osmotic potential levels for each parameter. The protein banding pattern was evaluated using gel imaging. The simulation of a water deficiency resulted in the perception of a stress and a subsequent response to NaCl in all of the parameters that were evaluated. The harsh effects of the NaCl were validated as the lack of the expression of low molecular weight proteins. Proteins with molecular weights of 110 and 30 kDa were indicative of water stress but not of salinity.

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Section: Introductionunclassified

Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Coelho¹,

Agostini²,

Guaberto³

et al. 2010

Acta Sci. Agron.

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“…Prolinerich proteins consist of a group of cell-wall proteins, which are distinguished by several repeated proline residues in their structures. It is known that the PRP genes are induced in developmental programs (JoseEstanyol et al 1992), biotic stresses such as pathogen attack (Sheng et al 1991), wounding (Keller 1993) and also abiotic stresses, such as salinity and water deficit (He et al 2002;Verdoy et al 2004), cold (Goodwin et al 1996) and heavy metals (Chai et al 1998). There are some suggestions in the literature for the roles of PRPs; for example, some (e.g.…”

Section: Discussionmentioning

confidence: 99%

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Roshandel

Flowers

2008

Plant Soil

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The effects of ionic stress on the physiology and gene expression of two rice genotypes (IR4630 and IR15324) that differ in salt tolerance, were investigated by evaluating changes in the biomass, Na + and K + concentrations and applying the cDNA-AFLP technique to highlight changes in gene expression. Over 8 days of salinisation, the effect of NaCl on the reduction of biomass (dry weight) was apparent from 24 h after salinisation (the first time point), indicating that the consequences of the build up of Na + (and Cl -) in the leaves of both lines was rapid. Furthermore, root growth of IR15324 was much more sensitive to salt than that of IR4630 (the reduction in root dry weight compared to nonsalinised plants was three times greater in IR15324 than IR4630). The two rice lines also differed in their Na + accumulation in saline conditions, a difference that was more marked in the shoots, particularly at the final harvest, than in the roots. Under salt stress, the K + content (µmol/shoot) increased over four successive harvests (24, 48, 96, 192 h) in both lines, but was always greater in IR4630 than in IR15324: differences in Na + /K + ratio appear to be an important determinant of salt tolerance in rice. To separate osmotic from ionic effects of salt, mannitol was applied as a non-ionic osmoticum at an osmotic potential estimated to be equivalent to 50 mM NaCl. Messenger RNA was sampled at 0.5, 6, 24, 48 and 192 hours after salinisation. Several products (AFLPbands) were detected, which were upregulated in the response to ionic effects of salt in the tolerant line (IR4630) and not expressed in the sensitive line (IR15324). Bioinformatic analysis indicated three of these AFLP-bands have a high-degree of sequence similarity with the genes encoding a proline rich protein, senescence associated protein and heat-shock protein. The data are novel in that they differentially highlight changes induced by the ionic rather than osmotic effects of salt and in a tolerant rather than a sensitive genotype. The possible roles of the products of these genes are discussed.

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“…Salinity clearly affects nodule formation and nitrogen fixation earlier and more severely than plant growth (Verdoy et al 2004;Fig. 2).…”

Section: Nodule Formation Structure and Physiologymentioning

confidence: 99%

Legumes in the reclamation of marginal soils, from cultivar and inoculant selection to transgenic approaches

Peña¹,

Pueyo²

2011

Agron. Sustain. Dev.

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Mineral nitrogen deficiency is a frequent characteristic of arid and semi-arid soils. Biological nitrogen fixation by legumes is a sustainable and environmentalfriendly alternative to chemical fertilization. Therefore, legumes have a high potential for the reclamation of marginal soils. Such issue is becoming more urgent due to the ever-rising requirement for food and feed, and the increasing extension of salinized and degraded lands, both as a consequence of global change and irrigation practices. This manuscript reviews current research on physiological and molecular mechanisms involved in the response and tolerance to environmental stresses of the Rhizobiumlegume symbiosis. We report in particular recent advances on the isolation, characterization, and selection of tolerant rhizobial strains and legume varieties, both by traditional methods and through biotechnological approaches. The major points are the following. (1) Understanding mechanisms involved in stress tolerance is advancing fast, thus providing a solid basis for the selection and engineering of rhizobia and legumes with enhanced tolerance to environmental constraints. (2) The considerable efforts to select locally adapted legume varieties and rhizobial inocula that can fix nitrogen under conditions of drought or salinity are generating competitive crop yields in affected soils. (3) Biotechnological approaches are used to obtain improved legumes and rhizobia with enhanced tolerance to abiotic stresses, paying particular attention to the sensitive nitrogen-fixing activity. Those biotechnologies are yielding transgenic crops and inocula with unquestionable potential.In conclusion, the role of legumes in sustainable agriculture, and particularly, their use in the reclamation of marginal lands, certainly has a very promising future.

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Differential organ‐specific response to salt stress and water deficit in nodulated bean (Phaseolus vulgaris)

Cited by 41 publications

References 71 publications

Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Legumes in the reclamation of marginal soils, from cultivar and inoculant selection to transgenic approaches

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