Differential occurrence of oxidative burst and antioxidative mechanism in compatible and incompatible interactions of Solanum lycopersicum and Ralstonia solanacearum

Mandal, Sumit; Das, Rupa Kumar; Mishra, Sanjeev

doi:10.1016/j.plaphy.2010.10.006

Cited by 60 publications

(37 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Biphasic ROS production with first minor burst and second major burst has been reported in several incompatible plantpathogen interactions (Mandal et al, 2011). With regards to incompatible interactions only, hydroxyl/ superoxide radicals are produced in a minor burst between 0 and 2 h and then followed by a major burst between 8 and 10 h postinoculation, immediately causing HR in host plants (Able et al, 1998).…”

Section: Discussionmentioning

confidence: 97%

Synergistic Biosynthesis of Biphasic Ethylene and Reactive Oxygen Species in Response to HemibiotrophicPhytophthora parasiticain Tobacco Plants

Park

2012

Plant Physiology

View full text Add to dashboard Cite

We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase in ROS and ethylene at 1 and 3 h (phase I), respectively, was followed by a second massive increase at 48 and 72 h (phase II), respectively, after pathogen inoculation. This biphasic pattern of ROS production significantly differed from the hypersensitive response exhibited by cryptogein-treated wild-type tobacco plants. The biphasic increase in ROS production was mediated by both NADPH oxidase isoforms, respiratory burst oxidase homolog (Rboh) D and RbohF. Conversely, different 1-aminocyclopropane-1-carboxylic acid synthase members were involved in specific phases of ethylene production: NtACS4 in the first phase and NtACS1 in the second phase. Biphasic production of ROS was inhibited in transgenic antisense plant lines expressing 1-aminocyclopropane-1-carboxylic acid synthase/oxidase or ethylene-insensitive3 as well as in transgenic plants impaired in ROS production. All tested transgenic plants were more tolerant against P. parasitica var nicotianae infection as determined based on trypan blue staining and pathogen proliferation. Further, silencing of NtACS4 blocked the second massive increase in ROS production as well as pathogen progression. Pathogen tolerance was due to the inhibition of ROS and ethylene production, which further resulted in lower activation of ROS-detoxifying enzymes. Accordingly, the synergistic inhibition of the second phase of ROS and ethylene production had protective effects against pathogen-induced cell damage. We conclude that the levels of ethylene and ROS correlate with compatible P. parasitica proliferation in susceptible plants.

show abstract

Section: Discussionmentioning

confidence: 97%

Synergistic Biosynthesis of Biphasic Ethylene and Reactive Oxygen Species in Response to HemibiotrophicPhytophthora parasiticain Tobacco Plants

Park

2012

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…Among the 15 cultivars the total phenol content was significantly enhanced upon pathogen inoculation, in which R cultivar (Arka Shirish) exhibited significant increase in phenols (3 folds) compared to control and least content was observed in MEBH 11 cultivar. The significant difference in phenols and polyphenols were described by Mandal et al [23] in response of F. oxysporum-tomato, Oidiumneo lycopersici-tomato interaction in susceptible genotypes of tomato.…”

Section: Total Phenol Assaymentioning

confidence: 72%

“…Isolates of R. solanacearum from soil, plant material and seeds were cultured using semi selective media and typical mucoid creamy white colonies with pink centers were observed as reported earlier [23][24][25][26][27][28] Further different isolates of R. solanacearum were further subjected to biochemical/physiological assays along with hypersensitive and pathogenicity tests [30,36,37]. These pathogens stained pink red for Gram's reaction and thin viscid mucoid strand for KOH solubility indicating gram negative nature.…”

Section: Bacterial Source and Identificationmentioning

confidence: 98%

“…Mandal et al [23] undertook to investigate the biochemical aspects of oxidative burst, anti-oxidative mechanism and cell wall reinforcement as initial defense responses of two tomato cultivars viz., Arka Meghali (susceptible) and BT-10 (resistant) against the devastating bacterial wilt pathogen R. solanacearum. The tomato variety BT-10 has been identified as resistant to R. solanacearum due to rapid increase in GPOD activity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biochemical and Molecular Variations of Guaiacol Peroxidase and Total Phenols in Bacterial Wilt Pathogenesis of Solanum melongena

Prakasha¹,

Umesha²

2016

Biochem Anal Biochem

View full text Add to dashboard Cite

Plants respond to bacterial pathogen attack by activating various defense responses, which are associated with the accumulation of several factors like defense-related enzymes and inhibitors which serve to prevent pathogen infection. The present study focused on the role of the defense-related enzyme and gene expression of Guaiacol preroxidase and Total phenol content in imparting activities was analyzed by selecting three different eggplant cultivars against bacterial wilt pathogen Ralstonia solanacearum. The temporal pattern of induction of these enzymes showed (42.72 U) maximum activity at 21 h after the pathogen inoculation (hpi) in resistant cultivars. The expressions of defense genes increased 5.5 folds in resistant eggplant cultivars after pathogen inoculation. The total phenol content increased significantly (P<0.05) in resistant cultivars upon pathogen inoculation compared to susceptible and highly susceptible cultivars. The biochemical and molecular markers provided an insight to understand the first line of defense responses in eggplant cultivars upon inoculation with the pathogen.

show abstract

“…An in vivo expression technology (IVET) screen performed on R. solanacearum during midphase tomato disease revealed that this bacterium encounters a stressful environment while in the host (7). Mutagenesis of stress response genes identified in this screen significantly decreased R. solanacearum's virulence on tomato (8).Host plants generate ROS in the form of hydrogen peroxide in response to infection by R. solanacearum (13,30), so this pathogen experiences oxidative stress during pathogenesis. The available R. solanacearum genomes predict multiple and redundant OSR genes (13), including an in planta-induced peroxidase, Bcp (7), and Dps (nonspecific DNA binding protein from starved cells), which is induced by tomato root exudates and is regulated by OxyR (9).…”

mentioning

confidence: 99%

Necessity of OxyR for the Hydrogen Peroxide Stress Response and Full Virulence in Ralstonia solanacearum

Flores-Cruz

Allen

2011

Appl Environ Microbiol

View full text Add to dashboard Cite

The plant pathogen Ralstonia solanacearum, which causes bacterial wilt disease, is exposed to reactive oxygen species (ROS) during tomato infection and expresses diverse oxidative stress response (OSR) genes during midstage disease on tomato. The R. solanacearum genome predicts that the bacterium produces multiple and redundant ROS-scavenging enzymes but only one known oxidative stress response regulator, OxyR. An R. solanacearum oxyR mutant had no detectable catalase activity, did not grow in the presence of 250 M hydrogen peroxide, and grew poorly in the oxidative environment of solid rich media. This phenotype was rescued by the addition of exogenous catalase, suggesting that oxyR is essential for the hydrogen peroxide stress response. Unexpectedly, the oxyR mutant strain grew better than the wild type in the presence of the superoxide generator paraquat. Gene expression studies indicated that katE, kaG, ahpC1, grxC, and oxyR itself were each differentially expressed in the oxyR mutant background and in response to hydrogen peroxide, suggesting that oxyR is necessary for hydrogen peroxideinducible gene expression. Additional OSR genes were differentially regulated in response to hydrogen peroxide alone. The virulence of the oxyR mutant strain was significantly reduced in both tomato and tobacco host plants, demonstrating that R. solanacearum is exposed to inhibitory concentrations of ROS in planta and that OxyRmediated responses to ROS during plant pathogenesis are important for R. solanacearum host adaptation and virulence.Plants produce reactive oxygen species (ROS) in response to invading pathogens, and an effective oxidative stress response (OSR) contributes to the fitness of phytopathogenic bacteria (18,39,40,51). However, research on the role of regulators of the OSR in the virulence of plant-pathogenic bacteria is limited and contradictory. In Agrobacterium tumefaciens, oxyR is necessary for tumorigenesis (34), but Erwinia chrysanthemi does not need oxyR for soft-rot virulence (32). OxyR is a redox-sensing LysR family transcriptional regulator that has been well characterized in several bacteria (23,43,52). In the absence of oxidative stress, OxyR is reduced and acts as a repressor of several genes, including oxyR itself (47, 52). In the presence of hydrogen peroxide, the conserved cysteines of OxyR (C199 and C208, in Escherichia coli) form a disulfide bond that changes its conformation and converts OxyR into a transcriptional activator (52, 53). In E. coli, OxyRregulated genes include catalase (kat), alkyl hydroperoxide reductase (ahp), glutaredoxin (grx), and glutathione reductase (gor) (53).The plant-pathogenic bacterium Ralstonia solanacearum causes bacterial wilt disease on many economically important crops, including tomato (19). Multiple quantitative virulence factors contribute to the disease (11); however, little is known about how R. solanacearum adapts to its host environment, which is a critical prerequisite for pathogen success. An in vivo expression technology (IVET) screen performed on R...

show abstract

Differential occurrence of oxidative burst and antioxidative mechanism in compatible and incompatible interactions of Solanum lycopersicum and Ralstonia solanacearum

Cited by 60 publications

References 38 publications

Synergistic Biosynthesis of Biphasic Ethylene and Reactive Oxygen Species in Response to HemibiotrophicPhytophthora parasiticain Tobacco Plants

Synergistic Biosynthesis of Biphasic Ethylene and Reactive Oxygen Species in Response to HemibiotrophicPhytophthora parasiticain Tobacco Plants

Biochemical and Molecular Variations of Guaiacol Peroxidase and Total Phenols in Bacterial Wilt Pathogenesis of Solanum melongena

Necessity of OxyR for the Hydrogen Peroxide Stress Response and Full Virulence in Ralstonia solanacearum

Contact Info

Product

Resources

About