Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments

Amelio, Daniela; Garofalo, Filippo; Brunelli, Elvira; Loong, Ai M.; Wong, Wai P.; Ip, Yuen K.; Tota, Bruno; Cerra, Maria Carmela

doi:10.1016/j.niox.2007.10.004

Cited by 57 publications

(28 citation statements)

References 48 publications

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“…The results shown in the current study, and previously by other groups (Tota et al, 2005;Amelio et al, 2006;Amelio et al, 2008;Jennings and Donald, 2008), strongly suggest that functional (possibly non-endothelial) NOS isoforms are expressed in several ectotherm species, belonging to dipnoi, teleosts, amphibians and reptiles, and that NO as a cardiovascular signaling molecule has been conserved in the evolution of vertebrates.…”

Section: Misfeldt a Fago And H Gessersupporting

confidence: 86%

“…Although endothelial NOS isoforms from non-mammalian species have not yet been isolated, recent evidence indicates the existence of functional NOS isoforms in the heart and vasculature of dipnoi, teleosts, reptiles and amphibians (e.g. Tota et al, 2005;Amelio et al, 2006;Amelio et al, 2008;Jennings and Donald, 2008). As an important general action, NO downregulates the O 2 consumption rate, primarily by inhibiting the binding of O 2 to mitochondrial cytochrome c oxidase both competitively and uncompetitively (Mason et al, 2006;Erusalimsky and Moncada, 2007;Cooper et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Nitric oxide increases myocardial efficiency in the hypoxia-tolerant turtle Trachemys scripta

Misfeldt

Fago

Gesser

2009

Journal of Experimental Biology

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Section: Misfeldt a Fago And H Gessersupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Nitric oxide increases myocardial efficiency in the hypoxia-tolerant turtle Trachemys scripta

Misfeldt

Fago

Gesser

2009

Journal of Experimental Biology

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“…In the past, the manifestation of organic structural modifications in aestivating animals has been largely neglected. However, recent reports reveal that aestivation in African lungfishes involves structural and functional modifications in the heart , the kidney (Ojeda et al, 2008;Amelio et al, 2008) and the intestine (Icardo et al, 2012). Indeed, there is an apparent increase in metabolic rate in African lungfishes during the induction phase of aestivation.…”

Section: Molecular Characterization Of F2 and Fgg From P Annectensmentioning

confidence: 99%

Aestivation induces changes in transcription and translation of coagulation factor II and fibrinogen gamma chain in the liver of the African lungfish,Protopterus annectens

Hiong

Tan

Boo

et al. 2015

Journal of Experimental Biology

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This study aimed to sequence and characterize two pro-coagulant genes, coagulation factor II ( f2) and fibrinogen gamma chain ( fgg), from the liver of the African lungfish Protopterus annectens, and to determine their hepatic mRNA expression levels during three phases of aestivation. The protein abundance of F2 and Fgg in the liver and plasma was determined by immunoblotting. The results indicated that F2 and Fgg of P. annectens were phylogenetically closer to those of amphibians than those of teleosts. Three days of aestivation resulted in an up-regulation in the hepatic fgg mRNA expression level, while 6 days of aestivation led to a significant increase (3-fold) in the protein abundance of Fgg in the plasma. Hence, there could be an increase in the blood-clotting ability in P. annectens during the induction phase of aestivation. By contrast, the blood-clotting ability in P. annectens might be reduced in response to decreased blood flow and increased possibility of thrombosis during the maintenance phase of aestivation, as 6 months of aestivation led to significant decreases in mRNA expression levels of f2 and fgg in the liver. There could also be a decrease in the export of F2 and Fgg from the liver to the plasma so as to avert thrombosis. Three to 6 days after arousal from 6 months of aestivation, the protein abundance of F2 and Fgg recovered partially in the plasma of P. annectens; a complete recovery of the transcription and translation of f2/F2 in the liver might occur only after refeeding.

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“…Nitrotyrosine is produced from tyrosine nitration that can be caused by peroxynitrite, a product of the reaction of superoxide with nitric oxide. It is thus interesting to note that nitric oxide synthase (NOS) is upregulated during estivation in some tissues of P. dolloi (Amelio et al 2008). Although no data has been reported for brain, it is possible that increased NOS activity in this tissue leads to elevated NO levels and increased peroxynitrite production.…”

mentioning

confidence: 99%

Upregulation of intracellular antioxidant enzymes in brain and heart during estivation in the African lungfish Protopterus dolloi

Page¹,

Salway²,

et al. 2009

J Comp Physiol B

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The African slender lungfish, Protopterus dolloi, is highly adapted to withstand periods of drought by secreting a mucous cocoon and estivating for periods of months to years. Estivation is similar to the diapause and hibernation of other animal species in that it is characterized by negligible activity and a profoundly depressed metabolic rate. As is typically observed in quiescent states, estivating P. dolloi are resistant to environmental stresses. We tested the hypothesis that P. dolloi enhances stress resistance during estivation by upregulating intracellular antioxidant defences in brain and heart tissues. We found that most of the major intracellular antioxidant enzymes, including the mitochondrial superoxide dismutase, cytosolic superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were upregulated in brain tissue of lungfish that had estivated for 60 days. Several of these enzymes were also elevated in heart tissue of estivators. These changes wer... Abstract The African slender lungWsh, Protopterus dolloi, is highly adapted to withstand periods of drought by secreting a mucous cocoon and estivating for periods of months to years. Estivation is similar to the diapause and hibernation of other animal species in that it is characterized by negligible activity and a profoundly depressed metabolic rate. As is typically observed in quiescent states, estivating P. dolloi are resistant to environmental stresses. We tested the hypothesis that P. dolloi enhances stress resistance during estivation by upregulating intracellular antioxidant defences in brain and heart tissues. We found that most of the major intracellular antioxidant enzymes, including the mitochondrial superoxide dismutase, cytosolic superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were upregulated in brain tissue of lungWsh that had estivated for 60 days. Several of these enzymes were also elevated in heart tissue of estivators. These changes were not due to food deprivation, as they did not occur in a group of Wsh that were deprived of food but maintained in water for the same period of time. We found little evidence of tissue oxidative damage in estivators. Products of lipid peroxidation (4-hydroxynonenal adducts) and oxidative protein damage (carbonylation) were similar in estivating and control lungWsh. However, protein nitrotyrosine levels were elevated in brain tissue of estivators. Taken together, these data indicate that estivating P. dolloi have enhanced oxidative stress resistance in brain and heart due to a signiWcant upregulation of intracellular antioxidant capacity.

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Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments

Cited by 57 publications

References 48 publications

Nitric oxide increases myocardial efficiency in the hypoxia-tolerant turtle Trachemys scripta

Nitric oxide increases myocardial efficiency in the hypoxia-tolerant turtle Trachemys scripta

Aestivation induces changes in transcription and translation of coagulation factor II and fibrinogen gamma chain in the liver of the African lungfish,Protopterus annectens

Upregulation of intracellular antioxidant enzymes in brain and heart during estivation in the African lungfish Protopterus dolloi

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