Differential nickel-induced responses of olfactory sensory neuron populations in zebrafish

Lazzari, Maurizio; Bettini, Simone; Milani, Liliana; Maurizii, Maria Gabriella

doi:10.1016/j.aquatox.2018.10.011

Cited by 22 publications

(24 citation statements)

References 89 publications

(135 reference statements)

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“…The genotoxic effect of the polluted environment in fishes (e.g., Lazzari et al 2017Lazzari et al , 2019 and bivalves was investigated applying different programs. In bivalve, for example, although a lot of genotoxicity biomarkers were examined in marine mussels, only a limited number were verified on a broad range of laboratory and field conditions (Dixon et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Attaallah

Marchionni

El-Beltagy

et al. 2020

The European Zoological Journal

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A huge progress has been achieved in mammalian in vitro technique. Instead, of the many trials to develop marine invertebrate cell cultures, only a few have obtained them and only from few tissues. Since in vitro cell culture of invertebrates could be very useful for many aspects of basic and applied science, in this work we investigate and describe the development of a technique for the establishment of cell cultures from gill, mantle and gonadic tissue of the Manila clam (Ruditapes philippinarum). We maintained viable cultures for up to 25 days. Culture viability and proliferation were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and with trypan blue, while an antibody against the ATP-dependent RNA helicase VASA, a protein expressed in the germline, and in multipotent stem cells of some animals, was used to verify the presence of these cell types. Following the described protocol: 1) explant resulted the better source to obtain cell cultures, when compared to enzymatic dissociation; 2) cultures of suspended cells were viable for longer period than adherent cells; 3) cell cultures obtained from tissues sampled in September-October performed better compared to other periods of the year, regarding maintenance and growth; 4) the tissue from which we obtained longer-lived cell cultures was gonadic tissue, especially form samples that show more undifferentiated germ cells and more VASA-stained cells. This study describes the challenges concerning the development of in vitro culture techniques for aquatic invertebrates.

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Section: Introductionmentioning

confidence: 99%

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Attaallah

Marchionni

El-Beltagy

et al. 2020

The European Zoological Journal

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“…Nickel (Ni) is a potent olfactory toxicant and widespread airborne and waterborne contaminant. Ni exposure induces anatomical disturbances in the olfactory epithelium and a reduction of both ciliated and microvillous OSNs in zebrafish [127]. Uranium (U) is a radioelement that can be found in contaminated water ecosystems both as its radioactive or depleted form.…”

Section: The Olfactory System Of Zebrafish As a Model Of Neuroplasmentioning

confidence: 99%

The Olfactory System of Zebrafish as a Model for the Study of Neurotoxicity and Injury: Implications for Neuroplasticity and Disease

Calvo-Ochoa

Byrd-Jacobs

2019

IJMS

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The olfactory system, composed of the olfactory organs and the olfactory bulb, allows organisms to interact with their environment and through the detection of odor signals. Olfaction mediates behaviors pivotal for survival, such as feeding, mating, social behavior, and danger assessment. The olfactory organs are directly exposed to the milieu, and thus are particularly vulnerable to damage by environmental pollutants and toxicants, such as heavy metals, pesticides, and surfactants, among others. Given the widespread occurrence of olfactory toxicants, there is a pressing need to understand the effects of these harmful compounds on olfactory function. Zebrafish (Danio rerio) is a valuable model for studying human physiology, disease, and toxicity. Additionally, the anatomical components of the zebrafish olfactory system are similar to those of other vertebrates, and they present a remarkable degree of regeneration and neuroplasticity, making it an ideal model for the study of regeneration, reorganization and repair mechanisms following olfactory toxicant exposure. In this review, we focus on (1) the anatomical, morphological, and functional organization of the olfactory system of zebrafish; (2) the adverse effects of olfactory toxicants and injury to the olfactory organ; and (3) remodeling and repair neuroplasticity mechanisms following injury and degeneration by olfactory toxicant exposure.

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“…Copper exposures to salmon have produced similar changes in expression of olfactory sensory genes, specifically those present in ciliated OSNs (Wang et al, 2013). Copper and nickel exposures in zebrafish appear to affect both ciliated and microvillus OSNs, but not crypt cell populations (Lazzari et al, 2017;Lazzari et al, 2018).…”

Section: Arsenic Exposure During Embryogenesis Reduces Response To Pheromones and Alters Levels Of Ciliated Osnsmentioning

confidence: 99%

Exposure to arsenic during embryogenesis impairs olfactory sensory neuron differentiation and function into adulthood

et al. 2019

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Arsenic is a contaminant of food and drinking water. Epidemiological studies have reported correlations between arsenic exposure and neurodevelopmental abnormalities, such as reduced sensory functioning, while in vitro studies have shown that arsenic reduces neurogenesis and alters stem cell differentiation. The goal of this study was assess whether arsenic exposure during embryogenesis reduced olfactory stem cell function and/or numbers, and if so, whether those changes persist into adulthood. Killifish (Fundulus heteroclitus) embryos were exposed to 0, 10, 50 or 200 ppb arsenite (As III ) until hatching, and juvenile fish were raised in clean water. At 0, 2, 4, 8, 16, 28 and 40 weeks of age, odorant response tests were performed to assess specific olfactory sensory neuron (OSN) function. Olfactory epithelia were then collected for immunohistochemical analysis of stem cell (Sox2) and proliferating cell numbers (PCNA), as well as the number and expression of ciliated (calretinin) and microvillus OSNs (G αi3 ) at 0, 4, 16 and 28 weeks. Odorant tests indicated that arsenic exposure during embryogenesis increased the start time of killifish responding to pheromones, and this altered start time persisted to 40 weeks postexposure. Response to the odorant taurocholic acid (TCA) was also reduced through week 28, while responses to amino acids were not consistently altered. Immunohistochemistry was used to determine whether changes in odorant responses were correlated to altered cell numbers in the olfactory epithelium, using markers of proliferating cells, progenitor cells, and specific OSNs. Comparisons between response to pheromones and PCNA+ cells indicated that, at week 0, both parameters in exposed fish were significantly reduced from the control group. At week 28, all exposure are still significantly different than control fish, but now with higher PCNA expression coupled with reduced pheromone responses. A similar trend was seen in the comparisons between Sox2-expressing progenitor cells and response to pheromones, although Sox2 expression in the 28 *

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Differential nickel-induced responses of olfactory sensory neuron populations in zebrafish

Cited by 22 publications

References 89 publications

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

The Olfactory System of Zebrafish as a Model for the Study of Neurotoxicity and Injury: Implications for Neuroplasticity and Disease

Exposure to arsenic during embryogenesis impairs olfactory sensory neuron differentiation and function into adulthood

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