Differential motile response of human malignant mesothelioma cells to fibronectin, laminin and collagen type IV: The role of β1 integrins

Klominek, Julius; Karuppan, Suchitra Sumitran; Hauzenberger, Dan

doi:10.1002/(sici)1097-0215(19970917)72:6<1034::aid-ijc19>3.0.co;2-4

Cited by 39 publications

(29 citation statements)

References 33 publications

(31 reference statements)

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“…Klominek and co-workers previously showed that integrins are expressed in MM cell lines and that they mediate migration to ECM proteins, including collagen type IV, fibronectin, and laminin [94]. Koukoulis et al studied 22 clinical specimens of MM and found that the expression of most integrins, including the a6b4 integrin, in epithelioid MM is similar to previously reported patterns in AC of lung, and often other origins [95].…”

Section: Adhesion Molecules and Other Membrane Receptorssupporting

confidence: 58%

New Diagnostic and Molecular Characteristics of Malignant Mesothelioma

Davidson

2008

Ultrastructural Pathology

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Malignant mesothelioma is a primary cancer of the serosal cavities, an anatomic site that is also frequently affected by metastatic disease, predominantly from primary carcinomas of the lung, breast, and ovary. Advances in immunohistochemistry have resulted in improved diagnostic sensitivity and specificity in the differential diagnosis between metastatic adenocarcinoma and malignant mesothelioma in both cytological and histological material. Recently, the author's group applied high throughput technology to the identification of new markers that may aid in differentiating malignant mesothelioma from ovarian and peritoneal serous carcinoma, tumors with closely related histogenesis and antigenic profile. In addition to the improved tools available for serosal cancer diagnosis, knowledge regarding the biology of malignant mesothelioma has been accumulating in recent years. This review presents current data regarding the diagnostic and biological aspects of malignant mesothelioma.

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Section: Adhesion Molecules and Other Membrane Receptorssupporting

confidence: 58%

New Diagnostic and Molecular Characteristics of Malignant Mesothelioma

Davidson

2008

Ultrastructural Pathology

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“…Klominek and co-workers previously showed that integrins are expressed in MM cell lines and that they mediate migration towards ECM proteins, including collagen type IV, fibronectin and laminin (Klominek, 1997). Koukoulis et al studied 22 clinical MM specimens and found that the expression of most integrins, including the α6β4 integrin, in epithelioid MM is similar to previously reported patterns in AC of the lung, and often of other origins (Koukoulis, 1997).…”

Section: Adhesion Molecules and Other Membrane Receptorsmentioning

confidence: 99%

Expression of Cancer-Associated Molecules in Malignant Mesothelioma

Davidson¹

2007

Biomark�Insights

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Malignant mesothelioma (MM) is a malignant tumor derived from mesothelial cells, native cells of the body cavities. Exposure to asbestos is the most strongly established etiologic factor, predominantly for the most common disease form, pleural mesothelioma. The pathogenesis of MM involves the accumulation of extensive cytogenetic changes, as well as cancer-related phenotypic alterations that facilitate tumor cell survival, invasion and metastasis. This review presents current knowledge regarding the biological characteristics of this disease that are linked to the so-called hallmarks of cancer. In addition, data suggesting that the anatomic site (solid tumor vs. effusion) affects the expression of metastasis-associated and regulatory molecules in MM are presented. Finally, recent work in which high-throughput methodology has been applied to MM research is reviewed. The data obtained in the reviewed research may aid in defi ning new prognostic markers and therapeutic targets for this aggressive disease in the future.

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“…Integrins and CD44 interact with an array of extracellular matrix (ECM) components, including collagen, fibronectin, laminin, vitronectin, and hyaluronan (HA), respectively (Etoh et al, 1992;Danen et al, 1993;Aznavoorian et al, 1996;Goebeler et al, 1996;Klominek et al, 1997), simultaneously acting as structural links from the cytoskeleton to the ECM as well as signaling molecules (Lokeshwar et al, 1994;Clark and Brugge, 1995;Entwistle et al, 1996;Cox and Huttenlocher, 1998).…”

Section: Introductionmentioning

confidence: 99%

Functional Hierarchy of Simultaneously Expressed Adhesion Receptors: Integrin α2β1 but Not CD44 Mediates MV3 Melanoma Cell Migration and Matrix Reorganization within Three-dimensional Hyaluronan-containing Collagen Matrices

Maaser

Wolf

Klein

et al. 1999

MBoC

116

119

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Haptokinetic cell migration across surfaces is mediated by adhesion receptors including ␤1 integrins and CD44 providing adhesion to extracellular matrix (ECM) ligands such as collagen and hyaluronan (HA), respectively. Little is known, however, about how such different receptor systems synergize for cell migration through three-dimensionally (3-D) interconnected ECM ligands. In highly motile human MV3 melanoma cells, both ␤1 integrins and CD44 are abundantly expressed, support migration across collagen and HA, respectively, and are deposited upon migration, whereas only ␤1 integrins but not CD44 redistribute to focal adhesions. In 3-D collagen lattices in the presence or absence of HA and cross-linking chondroitin sulfate, MV3 cell migration and associated functions such as polarization and matrix reorganization were blocked by anti-␤1 and anti-␣2 integrin mAbs, whereas mAbs blocking CD44, ␣3, ␣5, ␣6, or ␣v integrins showed no effect. With use of highly sensitive time-lapse videomicroscopy and computer-assisted cell tracking techniques, promigratory functions of CD44 were excluded. 1) Addition of HA did not increase the migratory cell population or its migration velocity, 2) blocking of the HA-binding Hermes-1 epitope did not affect migration, and 3) impaired migration after blocking or activation of ␤1 integrins was not restored via CD44. Because ␣2␤1-mediated migration was neither synergized nor replaced by CD44 -HA interactions, we conclude that the biophysical properties of 3-D multicomponent ECM impose more restricted molecular functions of adhesion receptors, thereby differing from haptokinetic migration across surfaces. INTRODUCTIONTumor cell invasion and migration are supported by different adhesion receptor systems, including integrins and CD44 (Stetler-Stevenson et al., 1993;Sherman et al., 1994; Friedl and Brö cker, 1999). Integrins and CD44 interact with an array of extracellular matrix (ECM) components, including collagen, fibronectin, laminin, vitronectin, and hyaluronan (HA), respectively (Etoh et al., 1992;Danen et al., 1993;Aznavoorian et al., 1996;Goebeler et al., 1996;Klominek et al., 1997), simultaneously acting as structural links from the cytoskeleton to the ECM as well as signaling molecules (Lokeshwar et al., 1994;Clark and Brugge, 1995;Entwistle et al., 1996;Cox and Huttenlocher, 1998).In human melanoma, the expression of ␣2␤1 integrin is correlated with metastatic behavior (Klein et al., 1991b) and is involved in melanoma cell migration on collagen surfaces (Etoh et al., 1992) and in the reorganization of collagen matrices (Klein et al., 1991b;Schiro et al., 1991;Riikonen et al., 1995). Likewise, ␣3␤1 and ␣v␤3 integrins mediate chemotactic and haptotactic motility toward their respective ligands collagen (Lauer et al., 1998) and vitronectin □ V Online version of this article contains video material. Online version available at www.molbiolcell.org. ‡ Corresponding author. E-mail address: peter.fr@mail.uniwuerzburg.de. Abbreviations used: CS, chondroitin sulfate; ECM, extracellular matri...

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Differential motile response of human malignant mesothelioma cells to fibronectin, laminin and collagen type IV: The role of β1 integrins

Cited by 39 publications

References 33 publications

New Diagnostic and Molecular Characteristics of Malignant Mesothelioma

New Diagnostic and Molecular Characteristics of Malignant Mesothelioma

Expression of Cancer-Associated Molecules in Malignant Mesothelioma

Functional Hierarchy of Simultaneously Expressed Adhesion Receptors: Integrin α2β1 but Not CD44 Mediates MV3 Melanoma Cell Migration and Matrix Reorganization within Three-dimensional Hyaluronan-containing Collagen Matrices

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