Differential modulation of β-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines

Derache, Chrystelle; Esnault, Evelyne; Bonsergent, Claire; Vern, Yves Le; Quéré, Pascale; Lalmanach, Anne-Christine

doi:10.1016/j.dci.2009.03.005

Cited by 43 publications

(29 citation statements)

References 42 publications

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“…Inbred chicken lines differing in cecal bacterial carriage showed marked differences in expression of AvBD1 and AvBD2, with higher expression in the line with the lower bacterial carriage (91). Salmonella infection interfered with AvBD2 expression in the cells from a susceptible, but not a resistant, line (101). Collectively, these studies reinforce the concept of an important role of the b-defensins in Salmonella resistance in chickens.…”

Section: Salmonellasupporting

confidence: 71%

Integrated Genomic Approaches to Enhance Genetic Resistance in Chickens

Cheng

Kaiser

Lamont

2013

Annu. Rev. Anim. Biosci.

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The chicken has led the way among agricultural animal species in infectious disease control and, in particular, selection for genetic resistance. The generation of the chicken genome sequence and the availability of other empowering tools and resources greatly enhance the ability to select for enhanced disease resistance via genetic markers and to understand more deeply the biological basis of host resistance. In this review, we discuss how integrated genomic approaches are able to identify specific genes and genetic markers associated with disease resistance, give select examples of contemporary work involving various genomic strategies to identify disease resistance genes, and finish by giving some final thoughts on predicted applications in the near future. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. AbstractThe chicken has led the way among agricultural animal species in infectious disease control and, in particular, selection for genetic resistance. The generation of the chicken genome sequence and the availability of other empowering tools and resources greatly enhance the ability to select for enhanced disease resistance via genetic markers and to understand more deeply the biological basis of host resistance. In this review, we discuss how integrated genomic approaches are able to identify specific genes and genetic markers associated with disease resistance, give select examples of contemporary work involving various genomic strategies to identify disease resistance genes, and finish by giving some final thoughts on predicted applications in the near future.

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Section: Salmonellasupporting

confidence: 71%

Integrated Genomic Approaches to Enhance Genetic Resistance in Chickens

Cheng

Kaiser

Lamont

2013

Annu. Rev. Anim. Biosci.

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“…Avian defensins have been linked to host resistance to Salmonella intestinal carriage (19,20). AvBD1 and AvBD2 have recently been shown to be expressed by chicken intestinal epithelial cells (5). Surprisingly, live Salmonella strain LA5 was shown to inhibit AvBD2 expression in this cell population, in spite of having relatively low susceptibility to this avian defensin.…”

Section: Vol 53 2009 Chicken Bone Marrow-derived ␤-Defensins 4651mentioning

confidence: 99%

Primary Structure and Antibacterial Activity of Chicken Bone Marrow-Derived β-Defensins

Derache

Labas

Aucagne

et al. 2009

Antimicrob Agents Chemother

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Three biologically active ␤-defensins were purified by chromatography from chicken bone marrow extract: avian ␤-defensin 1 (AvBD1), AvBD2, and the newly isolated ␤-defensin AvBD7. Mass spectrometry analyses showed that bone marrow-derived AvBD1, -2, and -7 peptides were present as mature peptides and revealed posttranslational modifications for AvBD1 and AvBD7 in comparison to their in silico-predicted amino acid sequences. Tandem mass spectrometry analysis using the nanoelectrospray-quadrupole time of flight method showed N-terminal glutaminyl cyclization of mature AvBD7 and C-terminal amidation of mature AvBD1 peptide, while posttranslational modifications were absent in bone marrow-derived mature AvBD2 peptide. Furthermore, mass spectrometry analysis performed on intact cells confirmed the presence of these three peptides in mature heterophils. In addition, the antibacterial activities of the three ␤-defensins against a large panel of gram-positive and -negative bacteria were assessed. While the three defensins displayed similar antibacterial spectra of activity against gram-positive strains, AvBD1 and AvBD7 exhibited the strongest activity against gram-negative strains in comparison to AvBD2.

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“…Defensins play a major role in both innate and adaptive immunity (2). They have been found to be constitutively or inducibly expressed by neutrophils and epithelial cells from many mammals and birds, including chicken (1,3,4). They display a wide range of microbicidal or microbistatic activities against Gram-negative and Gram-positive bacteria, fungi, and viruses (4).…”

mentioning

confidence: 99%

Initial Insights into Structure-Activity Relationships of Avian Defensins

Derache

Meudal

Aucagne

et al. 2012

Journal of Biological Chemistry

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Background: Avian defensins are antimicrobial peptides of a bird's immunity. Results: The target of chicken AvBD2 defensin is not chiral. Its structure is not amphipathic. The reduced and AvBD2-K31A forms dramatically decrease antibacterial activity. Conclusion: AvBD2 may disrupt the bacterial membrane through a nonchiral, nonspecific interaction. Significance: Knowledge of the structure-function relationships of avian defensins is a prerequisite for their use as alternatives to antibiotics.

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Differential modulation of β-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines

Cited by 43 publications

References 42 publications

Integrated Genomic Approaches to Enhance Genetic Resistance in Chickens

Integrated Genomic Approaches to Enhance Genetic Resistance in Chickens

Primary Structure and Antibacterial Activity of Chicken Bone Marrow-Derived β-Defensins

Initial Insights into Structure-Activity Relationships of Avian Defensins

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